discontinued

SM098A IFNG / Interferon gamma antibody

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Mouse anti Mouse, Rat IFNG / Interferon gamma DB1

SM098A

Product Description for IFNG / Interferon gamma

Mouse anti Mouse, Rat IFNG / Interferon gamma DB1.
Presentation: Azide Free
Product is tested for Frozen Sections, Enzyme Immunoassay, Functional assay, Western blot / Immunoblot.

Properties for IFNG / Interferon gamma

Product Category Primary Antibodies
Quantity 1 mg
Synonyms IFN-gamma, gamma IFN
Presentation Azide Free
Reactivity Ms, Rt
Applications C, E, FN, WB
Clonality Monoclonal
Clone DB1
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P01580
Immunogen:
Recombinant rat IFN gamma.
GeneID:
15978
Isotype control SM20A (for use in rat samples)
Application Immunohistochemistry on frozen sections.
ELISA.
Western Blot.
Functional Assays.
Background Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.
Concentration 2.0 mg/ml
General Readings
  1. Paineau J, Priestley C, Fabre J, Chevalier S, van der Meide P, Schellekens H, et al. Effect of recombinant interferon gamma and interleukin-2 and of a monoclonal antibody against interferon gamma on the rat immune response against heart allografts. J Heart Lung Transplant. 1991 May-Jun;10(3):424-30. PubMed PMID: 1906747.
  2. Paineau J, Priestley C, Fabre J, Chevalier S, van der Meide P, Jacques Y, et al. Effects of gamma interferon and interleukin-2, and of gamma-interferon antibodies, on the rat immune response against allografts. Transplant Proc. 1989 Feb;21(1 Pt 1):999-1001. PubMed PMID: 2495638.
  3. van der Meide PH, Dubbeld M, Vijverberg K, Kos T, Schellekens H. The purification and characterization of rat gamma interferon by use of two monoclonal antibodies. J Gen Virol. 1986 Jun;67 ( Pt 6):1059-71. PubMed PMID: 2423637.
  4. Keller R, Keist R, Van der Meide PH, Groscurth P, Aguet M, Leist TP. Induction, maintenance, and reinduction of tumoricidal activity in bone marrow-derived mononuclear phagocytes by Corynebacterium parvum. Evidence for the involvement of a T cell- and interferon-gamma-independent pathway of macrophage activation. J Immunol. 1987 Apr 1;138(7):2366-71. PubMed PMID: 3104463.
  5. Jacob CO, Holoshitz J, Van der Meide P, Strober S, McDevitt HO. Heterogeneous effects of IFN-gamma in adjuvant arthritis. J Immunol. 1989 Mar 1;142(5):1500-5. PubMed PMID: 2493048.
  6. Kingston AE, Bergsteinsdottir K, Jessen KR, Van der Meide PH, Colston MJ, Mirsky R. Schwann cells co-cultured with stimulated T cells and antigen express major histocompatibility complex (MHC) class II determinants without interferon-gamma pretreatment: synergistic effects of interferon-gamma and tumor necrosis factor on MHC class II induction. Eur J Immunol. 1989 Jan;19(1):177-83. PubMed PMID: 2493382.
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity chromatography on Protein A
Buffer System:
PBS containing 125mM Trehalose
State:
Lyophilized purified IgG
Azide Free
Reconstitution:
Restore with 0.5ml distilled water
Specificity
Specificity:
This antibody reacts with IFN-gamma. Clone DB-1 exhibits high affinity binding to the natural and recombinant forms and effectively neutralises the anti viral activity of IFN-gamma. It also blocks the induction of Ia antigen expression on murine keratinocytes. There is no cross-reaction with rodent IFN-alpha or IFN-beta or with human interferons.
Species:
Rat, Mouse.
Gene ID 15978

Accessory Products

Proteins and/or Positive Controls

Proteins for IFNG / Interferon gamma (15 products)

Catalog No. Species Pres. Purity   Source  

IFNG / Interferon gamma

IFNG / Interferon gamma Mouse > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
20 µg / €135.00
  OriGene Technologies, Inc.

IFNG / Interferon gamma

IFNG / Interferon gamma Mouse > 98 %
Preparation: .
Purity Detail: Purity is >98%, as determined by Coomassie stained SDS-PAGE.
E. coli
10 µg / €115.00
  OriGene Technologies, Inc.

IFNG / Interferon gamma

IFNG / Interferon gamma Mouse > 98 % pure by SDS-PAGE gel and HPLC analyses. E. coli
  OriGene Technologies GmbH

IFNG / Interferon gamma

IFNG / Interferon gamma Mouse > 98 % pure by SDS-PAGE gel and HPLC analyses. E. coli
  OriGene Technologies GmbH

IFNG / Interferon gamma

IFNG / Interferon gamma Mouse Purified > 98 % Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
E. coli
  OriGene Technologies GmbH

IFNG / Interferon gamma

IFNG / Interferon gamma Mouse Purified > 98 % Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
E. coli
  OriGene Technologies GmbH

IFNG / Interferon gamma

IFNG / Interferon gamma Mouse Purified > 98 % Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
E. coli
  OriGene Technologies GmbH

IFNG / Interferon gamma

IFNG / Interferon gamma Mouse
  Abnova Taiwan Corp.

IFNG / Interferon gamma

IFNG / Interferon gamma Mouse
  Abnova Taiwan Corp.

IFNG / Interferon gamma

IFNG / Interferon gamma Human Purified
  Novus Biologicals Inc.

IFNG / Interferon gamma

IFNG / Interferon gamma Human Purified
  Novus Biologicals Inc.

IFNG / Interferon gamma

IFNG / Interferon gamma Human Purified
  Novus Biologicals Inc.

IFNG / Interferon gamma

IFNG / Interferon gamma Mouse > 95 % E. coli
  PBL Assay Science

IFNG / Interferon gamma

IFNG / Interferon gamma Mouse > 95 % E. coli
  PBL Assay Science

IFNG / Interferon gamma

IFNG / Interferon gamma Mouse
  PBL Assay Science
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