Product Description for SPARC / Osteonectin
anti SPARC / Osteonectin.
Properties: Control Peptide
Presentation: Purified
Properties: Control Peptide
Presentation: Purified
Properties for SPARC / Osteonectin
| Product Category | Control Peptides |
|---|---|
| Quantity | 50 µg |
| Synonyms | BM-40, Basement-membrane protein 40, ON, Secreted protein acidic and rich in cysteine |
| Presentation | Purified |
| Clonality | Polyclonal |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Polyclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
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|---|---|
| Application | Incubate antibody neat with at least a 50 fold stoichiometric excess of blocking peptide at 37°C for 20 minutes (molecular weights of peptide and antibody are ~2.5 kDa and ~160 kDa, respectively). Antibody can then be diluted to a concentration suitable for Western blot. Example: 10 µl or 10 µg of rabbit anti-SPARC is added to 10 µg of blocking peptide for a total volume of 20 µl. The mixture is allowed to incubate for 20 minutes at 37°C prior to dilution in suitable buffer (for Western blot, etc.). |
| Background | SPARC is a key factor in cell-matrix interactions and possibly tumour aggressiveness. The SPARC gene, which encodes a multifunctional glycoprotein with roles in tissue development, remodelling and fibrosis. A regulator of cell-extracellular matrix (ECM) interactions, SPARC represents a major factor in the ECM remodelling occurring during tumour invasion. in silico analysis reveals 4 UTR-SNPs located in the 3 -UTR of the SPARC gene, corresponding to 1474 g a, 1551 g c, 1922 t g and 2072 c t changes, which are significantly associated with tumoral state of the tissue. Of all hits, the 2072 SPARC polymorphism had the best association with cancer. SPARC therefore is a gene involved in a number of diseases including rheumatoid arthritis, scleroderma, tumor development and metastasis. SPARC variants have been detected in tumour samples of patients with acute myeloblastic leukemia (AML). |
| Concentration | Lot specific |
| Storage | Store at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Format |
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| Specificity |
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| Gene ID | 6678 |
Accessory Products
Recommended Secondary Antibodies for SPARC / Osteonectin (0 products)
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