Carbonic anhydrase / can (1-220, His-tag) - AR03030PU-N

Product Description for Carbonic anhydrase / can

Carbonic anhydrase / can.
Presentation: Purified

Product Category	Proteins & Growth Factors
Quantity	0.1 mg
Synonyms	Carbonate dehydratase 2, Carbonic anhydrase 2, cynT2, yadF
Presentation	Purified
Molecular weight	27 kDa
Source	E. coli
Species (Protein)	E. coli
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Proteins (de)

Immunogen	Swiss Prot Num: P61517
Purity	> 95 % by SDS PAGE
Background	Carbonic anhydrase (CA) is an enzyme that catalyses rapid conversion of carbon dioxide to bicarbonate and protons (CO2 + H2O ↔ HCO3− + H+). Most carbonic anhydrases contain a zinc ion in their active site and the primary function of this enzyme is known to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide of tissues. Carbonic anhydrases have been found in all kingdoms of life.
Concentration	1.0 mg/ml (determined by Bradford assay)
General Readings	Sawaya MR., et al (2006) J Biol Chem.; 17;281(11):7546-55.
Storage	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description	Description: Recombinant carbonic anhydrase, fused to His-tag, was expressed in E.coli and purified by conventional chromatography techniques. AA Sequence: `MGSSHHHHHH SSGLVPRGSH MKDIDTLISN NALWSKMLVE EDPGFFEKLA QAQKPRFLWI GCSDSRVPAE RLTGLEPGEL FVHRNVANLV IHTDLNCLSV VQYAVDVLEV EHIIICGHYG CGGVQAAVEN PELGLINNWL LHIRDIWFKH SSLLGEMPQE RRLDTLCELN VMEQVYNLGH STIMQSAWKR GQKVTIHGWA YGIHDGLLRD LDVTATNRET LEQRYRHGIS NLKLKHANHK` Molecular weight: (240 aa), confirmed by MALDI-TOF
Format	Buffer System: 20mM Tris pH 8.0, 1mM DTT, 10 % glycerol Purity: >95% by SDS PAGE State: Liquid purified protein Purified