AR10580PU-S Hepatitis C Virus Core (2-192)

HCV is a small 50nm, enveloped, single-stranded, positive sense RNAvirus in the family Flaviviridae.
HCV has a high rate of replication with approximately one trillion particles produced each day in an infected individual. Due to lack of proofreading by the HCV RNA polymerase, the HCV has an exceptionally high mutation rate, a factor that may help it elude the host's immune response. Hepatitis C virus is classified into six genotypes(1-6) with several subtypes within each genotype. The preponderance and distribution of HCV genotypes varies globally. Genotype is clinically important in determining potential response to interferon-based therapy and the required duration of such therapy. Genotypes 1 and 4 are less responsive to interferon-based treatment than are the other genotypes (2, 3, 5 and 6).

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Description:

The E.coli derived recombinant protein contains the HCV core nucleocapsid genotype 1b, immunodominant regions, amino acids 2-192, 22 kDa.
The protein is fused with beta-Galactosidase (114 kDa) at N-terminus.
Specificity: Immunoreactive with sera of HCV-infected individuals.

Buffer System:

20mM Tris-HCl pH 8, 8M Urea and 10mM beta-Mercaptoethanol

Purity:

>95% pure as determined by 10% PAGE (coomassie staining).
Purification Method: HCV-Core protein was purified by proprietary chromatographic technique.

State:

Purified