14-3-3 protein beta/alpha antibody
Principal name
14-3-3 protein beta/alpha antibody
Alternative names for 14-3-3 protein beta/alpha antibody
YWHAB, KCIP-1, Protein 1054, Protein kinase C inhibitor protein 1
Ncbi ID
9606, NP_003395.1, NP_062250.1, NP_003395, NM_003404, NP_647539, NP_062250, NP_061223.2, NM_018753, NM_019377
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F), Immunoprecipitation (IP), Frozen Sections (C)
Background of 14-3-3 protein beta/alpha antibody
3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, Ï?, ζ, Ï?, and η that have been identified in mammals. The initially described α and δ isoforms are confirmed to be phosphorylated forms of β and ζ, respectively (3). Through their amino-termil α helical region, 14-3-3 proteins form homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kises, phosphatases, and other sigling molecules (3,4). The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser/Thr motif. However, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation-independent interactions, has been observed (4). 14-3-3 binding masks specific sequences of the target protein and therefore modulates target protein localization, phosphorylation state, stability, and molecular interactions (1-4). 14-3-3 proteins may also induce target protein conformatiol changes that modify target protein function (4,5). Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in development and in acute response to extracellular sigls and drugs, suggesting that 14-3-3 isoforms may perform different functions despite their sequence similarities (4). Several studies suggest that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes (2,3).
General readings
Rodriguez LG., et al., (2005). J Cell Physiol. Jan; 202(1):285-94.
Mils V , et al., (2000). Oncogene. Mar 2; 19(10):1257-65.
Immunohistochemistry (IHC) analyzes of 14-3-3 beta antibody (Cat.-No. AP06765PU-N) in paraffin-embedded human brain tissue.
Western blot (WB) analysis of 14-3-3 β antibody (Cat.-No. AP06765PU-N) at 1/500 dilution:
Lane 1: Hela cell lysate
Lane 2: SP2/0 cell lysate
Lane 3: PC12 cell lysate
Lane 1: Hela cell lysate
Lane 2: SP2/0 cell lysate
Lane 3: PC12 cell lysate
Immunoblot analysis of cell and tissue lysate. Lane 1 : HeLa cell lysate. Lane 2 : Mouse kidney.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with YWHAB polyclonal antibody ( Cat # PAB4033 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
The YWHAB polyclonal antibody ( Cat # PAB4033 ) is used in Western blot to detect YWHAB in Jurkat cell lysate.
Immunoblot analysis of cell and tissue lysates. Lane 1 : Hela cell lysate, lane 2 : mouse brain and lane 3 : rat brain.
Immunohistochemistry: AM09043PU-N 14-3-3 beta antibody staining of Formalin-Fixed, Paraffin-Embedded Human Thyroid at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Immunofluorescence analysis of 14-3-3 beta in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human BI antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Western blot analysis: The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 beta antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunohistochemistry: AM09043PU-N 14-3-3 beta antibody staining of Formalin-Fixed, Paraffin-Embedded Human Kidney at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Immunohistochemistry: AM09043PU-N 14-3-3 beta antibody staining of Formalin-Fixed, Paraffin-Embedded Human Brain, Cortex at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot analysis: Cell lysates of HeLa (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-Human 14-3-3 beta antibody (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
14-3-3 beta, 1-246 aa: 15% SDS-PAGE (3 μg)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
The anti-YWHAB Pab is used in Western blot to detect YWHAB in Jurkat cell lysate.
Human brain stained with Anti-14-3-3 beta antibody
Western Blot analysis of Jurkat cell lysate with 14-3-3-beta antibody
Immunohistochemical staining of YWHAQ on formalin fixed, paraffin embedded human brain with YWHAB polyclonal antibody.
Immunofluorescence of 21d mouse brain cryosections showing specific staining of the 14-3-3 protein when phosphorylated at Ser58 in green and DNA in blue.
Western blot of rat brainstem lysate showing specific immuno-labeling of the ~29k 14-3-3 protein phosphorylated at Ser58 (Control). The immunolabeling is blocked by the phosphopeptide used as the antigen (Phos-pep) but not by the corresponding dephosphopeptide (not shown).
Cell lysates of HeLa ( 30 µg ) were resolved by SDS-PAGE, transferred to NC membrane and probed with YWHAB monoclonal antibody, clone J2E9 ( Cat # MAB3207 ; 1 : 1000 ). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Human Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 14-3-3 beta antibody at 1/200 dilution.
Sample (30 µg of whole cell lysate). Lane A: A431. Lane B: H1299.Lane C: Hela. Lane D: Hep G2. Lane E: Molt-4. Lane F: Raji. 12% SDS PAGE. YWHAB antibody diluted at 1:10000.
Human Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 14-3-3 beta antibody at 1/200 dilution.
Sample (30 µg of whole cell lysate). A: Raji. 12% SDS PAGE. YWHAB antibody diluted at 1/10000.
Human Fetal Brain; WB Suggested Anti-YWHAB Antibody. . Titration: 1.0 ug/ml. . Positive Control: Fetal Brain; YWHAB antibody - middle region (ARP30641_P050) in Human Fetal Brain cells using Western Blot
Sample (20 ug of whole cell lysate). A: mouse brain. 12% SDS PAGE. TA308715 diluted at 1:10000.
Sample (30 ug of whole cell lysate). A: A431 . B: H1299. C: Hela. D: Hep G2 . E: Molt-4 . F: Raji. 12% SDS PAGE. TA308715 diluted at 1:10000.
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using 14-3-3 beta(TA308715) antibody at 1:500 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 14-3-3 beta (TA308715) antibody at 1:200 dilution.
Western blot of rat hippocampal (Hipp) lysate showing immunolabeling of the ~29k 14-3-3 protein.
Western blot analysis of extracts from HepG2 cells and Jurkat cells, using 14-3-3 ß antibody.The lane on the right is treated with the synthesized peptide.
Predicted band size: 28kDa. Positive control: HT-29 cell, Mouse brain tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 8%SDS-PAGE Lane1: HT-29 cell lysate Lane2: Mouse brain tissue lysate Lysates: 40 ug per lane Primary antibody: 1/750 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 1 minute)
Predicted band size: 28kDa. Positive control: HT-29, 293T and Hela cell, Mouse brain tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lane1: HT-29 cell lysate Lane2: Mouse brain tissue lysate Lane3: 293T cell lysate Lane4: Hela cell lysate Lysates: 40 ug per lane Primary antibody: 1/250 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 1 second)
Predicted cell location: Cytoplasm . Positive control: Human breast cancer tissue. Recommended dilution: 1/10-100 The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using YWHAB antibody at dilution 1/10, on the right is treated with the synthetic peptide (Original magnification: x200)
Predicted band size: 28 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lysate: 30 ug Primary antibody: 1/550 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 seconds)
Predicted band size: 28 kDa . Positive control: Hela cell and mouse kidney tissue lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lane1: Hela cell lysate Lane2: Mouse kidney tissue lysate Lysates: 30 ug per lane Primary antibody: 1/550 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds)
Predicted cell location: Cytoplasm, Nucleus. Positive control: Human lung cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using YWHAB antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x200)
Western blot analysis of extracts of various cell lines, using YWHAB antibody.
Immunohistochemistry of paraffin-embedded human kidney using YWHAB antibody at dilution of 1:200 (400x lens).
Immunofluorescence analysis of HeLa cell using YWHAB antibody.
Western Blot analysis of Jurkat cell lysate with 14-3-3-beta antibody
Human Brain stained with anti-14-3-3 beta antibody.
Western blot of rat brainstem lysate showing specific immuno- labeling of the ~29k 14-3-3 protein phosphorylated at Ser58 (Control). The immunolabeling is blocked by the phosphopeptide used as the antigen (Phos-pep) but not by the corresponding dephosphopeptide (not shown).
- Quick-Filters:
- Cited (1)
- Highly Validated (3)
- Overnight Delivery (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA309071 | 14-3-3 protein beta/alpha (C-term) antibody |
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| Rabbit Anti-14-3-3 Protein Antibody | |||||||
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Rabbit | IgG | Aff - Purified | Bov, Can, Chk, Hu, Ms, Prim, Rt, Xen, Ze | WB |
0.1 ml /
€405.00
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| OriGene Technologies, Inc. | |||||||
Published recently! |
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| TA308715 | 14-3-3 protein beta/alpha antibody |
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| Rabbit Polyclonal antibody to 14-3-3 beta (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide) | |||||||
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Rabbit | IgG | Aff - Purified | Hu, Ms | ICC/IF, P, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
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| AP23582PU-N | 14-3-3 protein beta/alpha (1-141) antibody |
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 |
Rabbit | Aff - Purified | Hu, Ze | ICC/IF, P, WB |
50 µl /
€425.00
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| OriGene Technologies GmbH | |||||||
| +3 additional images |
Highly validated antibody! |
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| AP23583PU-N | 14-3-3 protein beta/alpha (34-221) antibody |
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 |
Rabbit | Aff - Purified | Hu, Ze | ICC/IF, P, WB |
50 µl /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
| +3 additional images |
Highly validated antibody! |
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| TA309072 | 14-3-3 protein beta/alpha antibody |
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| Rabbit Anti-14-3-3 (Ser58) Antibody (Phospho-Specific) | |||||||
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Rabbit | IgG | Purified | Hu, Rt | P, WB |
0.1 ml /
€405.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA312477 | 14-3-3 protein beta/alpha antibody |
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| Rabbit polyclonal anti-14-3-3 beta antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA322851 | 14-3-3 protein beta/alpha antibody |
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| Anti-YWHAB Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| TA322852 | 14-3-3 protein beta/alpha antibody |
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| Anti-YWHAB Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA322853 | 14-3-3 protein beta/alpha antibody |
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| Anti-YWHAB Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA322854 | 14-3-3 protein beta/alpha antibody |
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| Anti-YWHAB Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA332392 | 14-3-3 protein beta/alpha antibody |
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| Rabbit anti-YWHAB Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| AP06765PU-N | 14-3-3 protein beta/alpha antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | E, ICC/IF, P, WB |
0.1 mg /
€370.00
|
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP15156PU-N | 14-3-3 protein beta/alpha antibody |
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Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300402 | 14-3-3 protein beta/alpha (transcript variant 2) |
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| Recombinant protein of human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide (YWHAB), transcript variant 2 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
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| OriGene Technologies, Inc. | |||||
| TP315940 | 14-3-3 protein beta/alpha (transcript variant 1) |
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| Recombinant protein of human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide (YWHAB), transcript variant 1 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR207535L2V | Lenti ORF particles, Ywhab (GFP-tagged ORF) - Rat tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide (Ywhab), 200 uL, >10^7 TU/mL |
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 |
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR207535L1V | Lenti ORF particles, Ywhab (Myc-DDK-tagged ORF) - Rat tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide (Ywhab), 200 uL, >10^7 TU/mL |
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200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||