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AAK1 antibody

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Principal name

AAK1 antibody

Alternative names for AAK1 antibody

AP2-associated protein kinase 1, KIAA1048

SwissProt ID

P0C1X8 (Rat), Q2M2I8 (Human), Q3UHJ0 (Mouse)

Gene ID

22848 (AAK1), 269774 (Aak1)

Ncbi ID

9606, NP_055726, NP_055726.3, NM_001040106, NM_014911, NP_001035195.1

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of AAK1 antibody

AP2-associated protein kinase 1 (Aak1) is a member of the Ark1/Prk1 subfamily of Ser/Thr protein kinases that are thought to regulate endocytosis by phosphorylating the accessory endocytic components. Aak1 interacts with and phosphorylates the mu2 subunit of the AP-2 complex, which promotes binding of the AP-2 to tyrosine based (Yxxf) internalization motif-containing receptors and subsequent receptor endocytosis. At least two isoforms of Aak1 are known to exist; the longer isoform contains an extended carboxy-terminus that contains an additional clathrin-binding domain. Overexpression of this long isoform or Aak1 depletion by RNA interference impairs transferrin recycling from the early/sorting endosome, suggesting that Aak1 functions at multiple steps of the endosomal pathway by regulating transferrin internalization and its recycling back to the plasma membrane.

General readings

Henderson DM, Conner SD, A novel AAK1 splice variant functions at multiple steps of the endocytic pathway. Mol Biol Cell. 2007 Jul;18(7):2698-706.

H00022848-M02 - AAK1
Western Blot analysis of AAK1 expression in transfected 293T cell line by AAK1 monoclonal antibody (M02), clone 4B10.Lane 1: AAK1 transfected lysate(52 KDa).Lane 2: Non-transfected lysate.
H00022848-B01 - AAK1
Western Blot analysis of AAK1 expression in transfected 293T cell line (H00022848-T01) by AAK1 MaxPab polyclonal antibody.Lane 1: AAK1 transfected lysate(52.14 KDa).Lane 2: Non-transfected lysate.
AP14706PU-N - AAK1
Western blot analysis of anti-AAK1 Antibody (C-term) in HepG2 cell line lysates (35ug/lane). AAK1 (arrow) was detected using the purified Pab.
PAB8529 - AAK1
Western blot analysis of AAK1 expression in NIH/3T3 cell lysate ( Lane 1 ) and rat kidney tissue lysate ( Lane 2 ) .
AP17859PU-N - AAK1
Western blot analysis of AAK1 Antibody (N-term) (AP17859PU-N) in Ramos cell line lysates (35ug/lane). AAK1 (arrow) was detected using the purified Pab.
C11150-1 - AAK1
Western blot analysis of extracts from COS7 cells, using AAK1 Antibody. The lane on the right is treated with the synthesized peptide.
AP22978PU-N - AAK1
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
AP22978PU-N - AAK1
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
AP22978PU-N - AAK1
Western blot analysis of Aak1 in A-20 lysate with Aak1 antibody (AP22978PU-N) at (A) 1 and (B) 2 µg/ml.
AP22987PU-N - AAK1
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
AP22987PU-N - AAK1
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
AP22987PU-N - AAK1
Immunocytochemistry in A-20 cells with Aak1 antibody (AP22987PU-N) at 5 µg/ml.
AP22987PU-N - AAK1
Western blot analysis of Aak1 in A-20 lysate with Aak1 antibody (AP22987PU-N) at (A) 1 and (B) 2 µg/ml.
ARP55101_P050 - AAK1
Mouse Thymus; WB Suggested Anti-Aak1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Thymus; Aak1 antibody - C-terminal region (ARP55101_P050) in Mouse Thymus cells using Western Blot
ARP55102_P050 - AAK1
Human 721_B; WB Suggested Anti-AAK1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 721_B cell lysate. There is BioGPS gene expression data showing that AAK1 is expressed in 721_B.; AAK1 antibody - C-terminal region (ARP55102_P050) in Human 721_B cells using Western Blot
TA306656 - AAK1
Western blot analysis of Aak1 in A-20 lysate with Aak1 antibody at (A) 1 and (B) 2 µg/ml.
TA306661 - AAK1
Western blot analysis of Aak1 in A-20 lysate with Aak1 antibody at (A) 1 and (B) 2 ug/mL.
TA306661 - AAK1
Immunocytochemistry of Aak1 in A-20 cells with Aak1 antibody at 5 ug/mL.
TA306661 - AAK1
Immunofluorescence of aak1 in human brain tissue with aak1 antibody at 20 ug/mL.
TA311428 - AAK1
(1ug/ml) staining of Human Amygdala lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA315187 - AAK1
Western blot analysis of extracts from COS7 cells, using AAK1 antibody.The lane on the right is treated with the synthesized peptide.
TA334561 - AAK1
WB Suggested Anti-AAK1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: 721_B cell lysateThere is BioGPS gene expression data showing that AAK1 is expressed in 721_B
TA347740 - AAK1
Western blot analysis on COS7 cell lysate using AAK1 Antibody
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AAK1 (N-term) antibody
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Hu, Ms, Rt ICC/IF, P, WB
50 µg / €425.00
  OriGene Technologies GmbH
+3 additional images

Highly validated antibody!

AAK1 (C-term) antibody
  Rabbit Polyclonal Aak1 Antibody  
Western blot analysis of Aak1 in A-20 lysate with Aak1 antibody at (A) 1 and (B) 2 µg/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.

AAK1 (N-term) antibody
  Rabbit Polyclonal Aak1 Antibody  
Western blot analysis of Aak1 in A-20 lysate with Aak1 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+2 additional images

AAK1 antibody
  Goat Anti-AAK1 Antibody  
(1ug/ml) staining of Human Amygdala lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Rt E, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

AAK1 antibody
  Rabbit polyclonal anti-AAK1 antibody  
Western blot analysis of extracts from COS7 cells, using AAK1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

AAK1 antibody
  Rabbit Polyclonal Anti-AAK1 Antibody  
Western blot analysis on COS7 cell lysate using AAK1 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €335.00
  OriGene Technologies, Inc.

AAK1 (C-term) antibody
Western blot analysis of anti-AAK1 Antibody (C-term) in HepG2 cell line lysates (35ug/lane). AAK1 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH

AAK1 (N-term) antibody
Western blot analysis of AAK1 Antibody (N-term) (AP17859PU-N) in Ramos cell line lysates (35ug/lane). AAK1 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH

AAK1 (C-term) antibody
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Hu, Ms, Rt P, WB
50 µg / €425.00
  OriGene Technologies GmbH
+2 additional images

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, AAK1 (mGFP-tagged)-Human AP2 associated kinase 1 (AAK1), 200 uL, >10^7 TU/mL
Lenti ORF particles, AAK1 (mGFP-tagged)-Human AP2 associated kinase 1 (AAK1), 200 uL, >10^7 TU/mL  
200 µl / €2,100.00
  OriGene Technologies, Inc.
 

10 Item(s)

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Available Filters

Product Type
  1. Human Lenti ORF Particle (1)
  2. Primary Antibody (9)
Clonality
  1. Polyclonal (9)
Target
  1. AAK1 (9)
Property
  1. C-term (3)
  2. N-term (3)
Reactivity
  1. Human (Hu) (9)
  2. Mouse (Ms) (7)
  3. Rat (Rt) (7)
  4. Bovine (Bov) (1)
Applications
  1. Western blot / Immunoblot (WB) (9)
  2. Enzyme Immunoassay (E) (4)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (2)
  4. Paraffin Sections (P) (2)
Presentation
  1. Purified (5)
  2. Aff - Purified (3)
Host
  1. Rabbit (8)
  2. Goat (1)
Images
  1. 1 image (9)
  2. 3+ images (3)
Images: Applications
  1. Western blot / Immunoblot (WB) (9)
  2. Paraffin Sections (P) (2)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  4. Immunofluorescence (IF) (1)
Manufacturer
  1. OriGene Technologies (10)
Service
  • OriGene Technologies GmbH
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