AATF antibody
Principal name
AATF antibody
Alternative names for AATF antibody
CHE1, DED, HSPC277, CHE-1, Apoptosis-antagonizing transcription factor, Rb-binding protein Che-1
Ncbi ID
NP_036270, 9606, NP_036270.1, NM_019816, NM_053720, NP_062790.1
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)
Background of AATF antibody
AATF (apoptosis antagonizing transcription factor) was initially discovered as an interaction partner of ZIP kinase (ZIPK) (1), a member of death-associated protein (DAP) kinase family of pro-apoptotic serine/threonine kinases (2). AATF is a phosphoprotein containing an acidic region and a putative leucine zipper domain and nuclear localization signal, features which are typical of transcription factors. AATF inhibits the ZIPK-mediated pro-apoptotic pathway and may activate other anti-apoptotic pathways (3). Recently, it has also been shown to protect neural cells against oxidative damage induced by amyloid b-peptide and to inhibit aberrant production of the b-peptide by interacting with Par-4 (prostate apoptosis response-4), another pro-apoptotic leucine zipper protein that is associated with neuronal degeneration in Alzheimer’s disease (AD) (4,5), suggesting that AATF may have potential therapeutic applications in both familial and sporadic forms of AD.
General readings
Burgdorf,S., et al., (2004) J. Biol. Chem. 279 (17), 17524-17534
Human Intestine; AATF antibody - N-terminal region (ARP31867_P050) in Human Intestine cells using Immunohistochemistry
Human Liver; AATF antibody - N-terminal region (ARP31867_P050) in Human Liver cells using Immunohistochemistry
Human kidney; WB Suggested Anti-AATF Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Human kidney; AATF antibody - N-terminal region (ARP31867_P050) in Human kidney cells using Western Blot
Immunofluorescence of monoclonal antibody to AATF on HeLa cell. [antibody concentration 10 ug/ml]
AATF monoclonal antibody (M09), clone 2H6 Western Blot analysis of AATF expression in HeLa ( Cat # L013V1 ).
AATF polyclonal antibody (A01), Lot # AUH0060323QCS1 Western Blot analysis of AATF expression in PC-12 ( Cat # L012V1 ).
Human Daudi; WB Suggested Anti-AATF Antibody Titration: 5.0ug/ml. ELISA Titer: 1:312500. Positive Control: Daudi cell lysate. AATF is supported by BioGPS gene expression data to be expressed in Daudi.; AATF antibody - N-terminal region (ARP38957_T100) in Human Daudi cells using Western Blot
Human kidney; WB Suggested Anti-AATF Antibody Titration: 0.25ug/ml. ELISA Titer: 1:312500. Positive Control: Human kidney; AATF antibody - C-terminal region (ARP38958_T100) in Human kidney cells using Western Blot
Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Testis.
Immunoperoxidase of monoclonal antibody to AATF on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
Immunofluorescence of monoclonal antibody to AATF on HeLa cell. [antibody concentration 10 ug/ml]
AATF monoclonal antibody (M04), clone 3C7 Western Blot analysis of AATF expression in Hela NE ( Cat # L013V3 ).
Immunohistochemistry of AATF in human small intestine tissue with AATF polyclonal antibody ( Cat # PAB12886 ) at 10 µg/ml.
Western blot analysis of AATF in human small intestine tissue lysate with AATF polyclonal antibody ( Cat # PAB12886 ) at 0.5, 1 and 2 µg/ml.
Immunohistochemical analysis of AATF staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of AATF staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of AATF expression in HUVEC (A); HeLa (B) whole cell lysates.
Western blot analysis of AATF in human small intestine cell lysate with AATF antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.
Immunohistochemistry of AATF in human small intestine tissue with AATF antibody at 10 ug/ml.
Immunofluorescence of AATF in Human Small Intestine cells with AATF antibody at 20 ug/mL.
Western blot analysis of extracts from HeLa cells, HepG2 cells, COLO205 cells and HUVEC cells, using AATF antibody.The lane on the right is treated with the synthesized peptide.
WB Suggested Anti-AATF Antibody Titration: 5.0 ug/ml; ELISA Titer: 1: 312500; Positive Control: Daudi cell lysate. AATF is supported by BioGPS gene expression data to be expressed in Daudi
WB Suggested Anti-AATF Antibody Titration: 0.25 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human kidney
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Mouse heart tissue, 231 cells, Primary antibody: (AATF Antibody) at dilution 1/667, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
WB Suggested Anti-AATF Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: Human kidney
Human Intestine
Human Liver
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA306107 | AATF antibody |
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| Rabbit Polyclonal AATF Antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms | E, ICC/IF, P, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA312747 | AATF antibody |
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| Rabbit polyclonal anti-AATF antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA350967 | AATF antibody |
||||||
| Rabbit Polyclonal Anti-AATF Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP07651PU-N | AATF antibody |
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 |
Rabbit | Purified | Hu, Ms | P, WB |
50 µg /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP710286 | AATF (full length, C-term DDK tag) |
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| Purified recombinant protein of Human apoptosis antagonizing transcription factor (AATF), full length, with C-terminal DDK tag, expressed in sf9, 20ug | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
SF9 cells |
20 µg /
€411.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR208444L2V | Lenti ORF particles, Aatf (GFP-tagged) - Mouse apoptosis antagonizing transcription factor (Aatf), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€1,220.00
|
|
| OriGene Technologies, Inc. | ||
| MR208444L1V | Lenti ORF particles, Aatf (Myc-DDK-tagged) - Mouse apoptosis antagonizing transcription factor (Aatf), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,090.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR203248L1V | Lenti ORF particles, Aatf (Myc-DDK-tagged ORF) - Rat apoptosis antagonizing transcription factor (Aatf), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,090.00
|
|
| OriGene Technologies, Inc. | ||
