ABAT antibody
Principal name
ABAT antibody
Alternative names for ABAT antibody
4-aminobutyrate aminotransferase, Gamma-amino-N-butyrate transaminase, L-AIBAT, GABAT, GABA aminotransferase
Ncbi ID
9606, NP_065737, AAH08990, NP_000654.2, NP_000654, NP_001120920, NP_001164449.1, NM_172961, NM_031003, NM_000663, NM_020686, NM_001127448
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of ABAT antibody
4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities.4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene.
General readings
Inada,T., (2008) Pharmacogenet. Genomics 18 (4), 317-323
ABAT MaxPab polyclonal antibody. Western Blot analysis of ABAT expression in human liver.
Western Blot analysis of ABAT expression in transfected 293T cell line (H00000018-T01) by ABAT MaxPab polyclonal antibody.Lane 1: ABAT transfected lysate(55 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of ABAT expression in transfected 293T cell line (H00000018-T02) by ABAT MaxPab polyclonal antibody.Lane 1: ABAT transfected lysate(56.40 KDa).Lane 2: Non-transfected lysate.
Hum. Fetal Muscle; Host:Rabbit. Target Name:ABAT. Sample Tissue:Human Fetal Muscle. Antibody Dilution: 1.0ug/ml.; ABAT antibody - middle region (ARP51766_P050) in Hum. Fetal Muscle cells using Western Blot
Human Placenta; WB Suggested Anti-ABAT Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Human Placenta; ABAT antibody - middle region (ARP51766_P050) in Human Placenta cells using Western Blot
Transfected 293T; WB Suggested Anti-ABAT Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Transfected 293T; ABAT antibody - middle region (ARP51765_P050) in Transfected 293T cells using Western Blot
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ABAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806896)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ABAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806896)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABAT (RC218980, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ABAT.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ABAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806898)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ABAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806898)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABAT (RC218980, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ABAT.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ABAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806964)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ABAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806964)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABAT (RC218980, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ABAT.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABAT (RC218980, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ABAT.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ABAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806966)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ABAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806966)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABAT (RC218980, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ABAT.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ABAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806967)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ABAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806967)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABAT (RC218980, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ABAT.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ABAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806968)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ABAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806968)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ABAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806968)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABAT (RC218980, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ABAT.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ABAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806969)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ABAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806969)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ABAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806969)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABAT (RC218980, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ABAT.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ABAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806970)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABAT (RC218980, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ABAT.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ABAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806972)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ABAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806972)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABAT (RC218980, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ABAT.
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-ABAT mouse monoclonal antibody (UM800070). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.(1:100)
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-ABAT mouse monoclonal antibody (UM800071). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.(1:100)
Western blot analysis of extracts of various cell lines, using ABAT antibody.
Immunohistochemical of paraffin-embedded mouse lung using ABAT antibody at dilution of 1:200 (200x lens).
Immunohistochemical of paraffin-embedded human lung using ABAT antibody at dilution of 1:200 (200x lens).
Immunofluorescence analysis of A549 cell using ABAT antibody. Blue: DAPI for nuclear staining.
WB Suggested Anti-ABAT Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Transfected 293T
WB Suggested Anti-ABAT Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: Human Placenta
Host: Rabbit; Target Name: ABAT; Sample Tissue: Human Fetal Muscle; Antibody Dilution: 1.0 ug/ml
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA332592 | ABAT antibody |
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| Rabbit anti-ABAT Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 mg /
€335.00
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| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
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