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ABCA1 antibody

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Principal name

ABCA1 antibody

Alternative names for ABCA1 antibody

ABC1, ABC-1, CERP, ATP-binding cassette sub-family A member 1

SwissProt ID

O95477 (Human), P41233 (Mouse)

Gene ID

19 (ABCA1), 11303 (Abca1)

Ncbi ID

10090, 9606, NP_005493, NP_005493.2

Available reactivities

Ms (Mouse), Rt (Rat), Hu (Human), Hst (Hamster), Chk (Chicken), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rat, Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Flow Cytometry (F), Paraffin Sections (P), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of ABCA1 antibody

ABCA1 (ATP-binding cassette, sub-family A (ABC1), member 1), also known as ABC1, the cholesterol efflux regulatory protein (CERP), is a protein which in humans is encoded by the ABCA1 gene. The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes,contains 2,261 amino acids. Dot blot analysis of 50 tissues revealed ubiquitous expression of ABCA1 mRNA, with highest expression in placenta, liver, lung, adrenal glands, and all fetal tissues examined, and lowest expression in kidney, pancreas, pituitary, mammary gland, and bone marrow. This protein is a member of the ABCA subfamily. Members of the ABCA subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesterolefflux  pump in the cellular lipid removal pathway. Using human ABCA1 expressed in the membrane fraction of sf9 insect cells, Szakacs et al. found specific, Mg(2+)-dependent ATP binding and low basal ATPase activity. Addition of potential lipid substrates or lipid acceptors did not modify the ATPase activity or nucleotide occlusion by ABCA1. Szakacs et al. speculated that ABCA1 may be a regulatory protein or may require other protein partners for full activation.

MAB2326 - ABCA1
Western blot analysis of Abca1 in wild type liver tissue ( in RIPA ) using Abca1 monoclonal antibody, clone HJ1 ( Cat # MAB2326 ).Lane 1, KO mouse liver tissue.Lane 2, WT mouse liver tissue.
MAB2419 - ABCA1
FACS analysis of Raw ( red ) and Raw-cAMP ( blue ) cells using Abca1 monoclonal antibody, clone 5A1-1422.11 ( Cat # MAB2419 ) at a dilution of 1 : 4,000.
MAB2420 - ABCA1
Western blot analysis of ABCA1 using ABCA1 monoclonal antibody, clone 3A1.891.3 ( Cat # MAB2420 ) at various dilutions in ABCA1 transfected HeLa lysates.
PAB12504 - ABCA1
Detection of ABCA1 in mouse peritoneal macrophages using ABCA1 polyclonal antibody ( Cat # PAB12504 ). Lane 1 : T09 uninduced lysate, Lane 2 : T09 induced lysate.
AM20330PU-N - ABCA1
AM20330PU-N ABCA1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Skeletal Muscle at 10 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen.
ARP43660_P050 - ABCA1
Mouse Kidney; WB Suggested Anti-Abca1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Mouse Kidney; Abca1 antibody - middle region (ARP43660_P050) in Mouse Kidney cells using Western Blot
CPA2926-100ul - ABCA1
Western blot analysis of ABCA1 expression in A549 (A); Raw264.7 (B); PC12 (C) whole cell lysates.
TA309555 - ABCA1
Western Blot: ABCA1 Antibody - Western blot analysis of RAW 264.7 Whole cell Lysate Kit - Samples: 25ug whole cell lysate of RAW 264.7 cells treated with T0901317 (lane 1) and untreated (lane 2).
TA309555 - ABCA1
Western Blot: ABCA1 Antibody - Western Blot analysis of ABCA1. Samples: 40 ug of total cell post-nuclear lysate of raw macrophages treated with 9-cisretinoic acid and 22R-hydroxycholesterol.
TA309555 - ABCA1
Western Blot: ABCA1 antibody - Detection of ABCA1 in mouse peritoneal macrophages using anti-ABCA1 (Lot L). ECL exposure, 1 min. Lane 4: T09 uninduced lysate Lane 5: T09 induced lysate
TA309555 - ABCA1
Immunocytochemistry: ABCA1 Antibody - ABCA1 antibody was tested at 1:100 in HeLa cells with FITC (green). alpha-Tubulin antibody was tested at a 1:500 dilution using Mouse IgG secondary antibody at 1:1000 [HiLyte Fluor 555] (red). Nuclei were counterstained with Dapi (blue).
TA309556 - ABCA1
Western Blot: ABCA1 Antibody - Detection of ABCA1 in wildtype liver tissue (in RIPA) Lane 1: KO mouse liver tissue. Lane 2: WT mouse liver tissue.
TA309556 - ABCA1
Immunohistochemistry: ABCA1 Antibody (HJ1) - Astrocyte Marker - IHC analysis of ABCA1 in human renal cancer using DAB with hematoxylin counterstain.
TA309942 - ABCA1
Western blot analysis of extracts from Jurkat cells, using EMR1 antibody.The lane on the right is treated with the synthesized peptide.
TA310028 - ABCA1
Western blot analysis of mouse liver lysate (Lanes 1 and 2: 150ug lysate) and Jurkat cells (Lanes 3 and 4: 105ug) with anti-ABCA1 antibody diluated at 1:2000. (Predicted band size: 254kDa; observed band size: 120~130kDa)
TA322600 - ABCA1
Predicted band size: 254 kDa . Positive control: Raw264.7 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lysate: 30 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
TA332061 - ABCA1
WB Suggested Anti-Abca1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: Mouse Kidney
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  2. Highly Validated (1)

7 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ABCA1 antibody
  Rabbit Polyclonal ABCA1 Antibody  
Western Blot: ABCA1 Antibody - Western blot analysis of RAW 264.7 Whole cell Lysate Kit - Samples: 25ug whole cell lysate of RAW 264.7 cells treated with T0901317 (lane 1) and untreated (lane 2). Rabbit Purified Hst, Hu, Ms, Rt ICC/IF, WB
0.1 ml / €475.00
  OriGene Technologies, Inc.
+3 additional images

Highly validated antibody!

ABCA1 antibody
  Mouse Monoclonal ABCA1 Antibody (HJ1) - Astrocyte Marker  
Western Blot: ABCA1 Antibody - Detection of ABCA1 in wildtype liver tissue (in RIPA) Lane 1: KO mouse liver tissue. Lane 2: WT mouse liver tissue. Mouse HJ1 Purified Hu, Ms, Rt C, WB
0.1 ml / €415.00
  OriGene Technologies, Inc.
+1 additional image

ABCA1 antibody
  Mouse anti-ABCA1 monoclonal antibody  
Western blot analysis of extracts from Jurkat cells, using EMR1 antibody.The lane on the right is treated with the synthesized peptide. Mouse IgG AB.H10 Purified Chk, Hu, Ms E, IP, P, WB
0.1 mg / €260.00
  OriGene Technologies, Inc.

ABCA1 antibody
  Rabbit anti-ABCA1 polyclonal antibody  
Western blot analysis of mouse liver lysate (Lanes 1 and 2: 150ug lysate) and Jurkat cells (Lanes 3 and 4: 105ug) with anti-ABCA1 antibody diluated at 1:2000. (Predicted band size: 254kDa; observed band size: 120~130kDa) Rabbit IgG Purified Hu, Ms WB
0.1 ml / €195.00
  OriGene Technologies, Inc.

ABCA1 antibody
  Anti-ABCA1 Rabbit Polyclonal Antibody  
Predicted band size: 254 kDa . Positive control: Raw264.7 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lysate: 30 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

ABCA1 (1800-2260) antibody
ABCA1 Mouse IgG1 AB1.G6 Aff - Purified Hu E, IP, P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH

ABCA1 antibody
ABCA1 Mouse IgG HJ1 Purified Ms, Rt WB
0.1 mg / €360.00
  Neuromics Antibodies
+2 additional images

7 Item(s)

per page

Available Filters

Product Type
  1. Primary Antibody (7)
Clonality
  1. Monoclonal (4)
  2. Polyclonal (3)
Target
  1. ABCA1 (7)
Property
  1. 1800-2260 (1)
Reactivity
  1. Human (Hu) (6)
  2. Mouse (Ms) (6)
  3. Rat (Rt) (4)
  4. Chicken (Chk) (1)
  5. Hamster (Hst) (1)
Applications
  1. Western blot / Immunoblot (WB) (7)
  2. Enzyme Immunoassay (E) (2)
  3. Immunoprecipitation (IP) (2)
  4. Paraffin Sections (P) (2)
  5. Frozen sections (C) (1)
  6. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Presentation
  1. Purified (6)
  2. Aff - Purified (1)
Host
  1. Mouse (4)
  2. Rabbit (3)
Images
  1. 1 image (6)
  2. 3+ images (2)
Images: Applications
  1. Western blot / Immunoblot (WB) (5)
  2. Immunofluorescence (IF) (1)
  3. IHC (1)
Manufacturer
  1. OriGene Technologies (6)
  2. Neuromics Antibodies (1)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
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  • orders: ordering-de@origene.com
 
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