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ABCG1 antibody

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Principal name

ABCG1 antibody

Alternative names for ABCG1 antibody

ABC8, WHT1, ATP-binding cassette sub-family G member 1, White protein homolog

SwissProt ID

P45844 (Human), Q64343 (Mouse)

Gene ID

9619 (ABCG1), 11307 (Abcg1)

Ncbi ID

9606, NP_004906, NP_004906.3, NP_058198, NP_033723.1, NM_016818, NM_207174, NM_207629, NM_004915, NM_207628

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Frozen Sections (C), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F)

Background of ABCG1 antibody

ABCG1(Atp-binding cassette, subfamily g, member 1) also known as ABC8, ABC TRANSPORTER 8 or WHITE, DROSOPHILA, HOMOLOG OF, is a protein that in humans is encoded by the ABCG1 gene. ABCG1 is a regulator of macrophage cholesterol and phospholipid transport. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters.The ABCG1 gene is mapped on 21q22.3. This protein is a member of the White subfamily. It is involved in macrophage cholesterol and phospholipids transport, and may regulate cellular lipid homeostasis in other cell types. ABCG1 is induced in monocyte-derived macrophages during cholesterol influx mediated by acetylated low-density lipoprotein. Inhibition of ABCG1 protein expression using an antisense strategy resulted in reduced HDL3-dependent efflux of cholesterol and choline-phospholipids. In macrophages, silencing of GPS2 by RNA interference reduced ABCG1 expression and diminished ABCG1-mediated cholesterol efflux.

H00009619-M01 - ABCG1
Detection limit for recombinant GST tagged ABCG1 is approximately 0.1ng/ml as a capture antibody.
PAB12503 - ABCG1
Detection of ABCG1 in mouse peritoneal macrophages ( 40 µg ) using ABCG1 polyclonal antibody ( Cat # PAB12503 ). Lane 1 : positive ABCG1 control, lane 2 : 10 µM 22- ( R ) -hydrocholesterol treated macrophages, Lane 3 : 100 nM T0901713 ( LXRa ligand ) treated macrophages, and Lane 4 : 500 nM T091713 ( LXRa ligand ) treated macrophages.
AP17071PU-N - ABCG1
AP17071PU-N ABCG1 Antibody staining of Formalin-fixed and paraffin-embedded human hepatocarcinoma using peroxidase followed by DAB staining.
AP17071PU-N - ABCG1
Western blot analysis of ABCG1 Antibody (Center) in 293 cell line lysates (35ug/lane). ABCG1 (arrow) was detected using the purified Pab.
AP17071PU-N - ABCG1
Flow Cytometry: AP17071PU-N ABCG1 Antibody staining of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CPA2410-100ul - ABCG1
Western blot analysis of ABCG1 expression in HepG2 (A) whole cell lysates.
TP306478 - ABCG1
TA322701 - ABCG1
Predicted band size: 76 kDa. Positive control: HT-29 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes)
TA322701 - ABCG1
Predicted cell location: Cytoplasm . Positive control: Human gastric cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human gastric cancer tissue using ABCG1 antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x200)
TA322702 - ABCG1
Predicted cell location: Cytoplasm . Positive control: Human breast and colon cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using ABCG1 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x 200)
TA322702 - ABCG1
Predicted cell location: Cytoplasm . Positive control: Human breast and colon cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using ABCG1 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x200)

4 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ABCG1 antibody
  Anti-ABCG1 Rabbit Polyclonal Antibody  
Predicted band size: 76 kDa. Positive control: HT-29 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

ABCG1 antibody
  Anti-ABCG1 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm . Positive control: Human breast and colon cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using ABCG1 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

ABCG1 antibody
AP17071PU-N ABCG1 Antibody staining of Formalin-fixed and paraffin-embedded human hepatocarcinoma using peroxidase followed by DAB staining. Rabbit Ig Purified Hu F, P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+2 additional images

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ABCG1 (transcript variant 2)
  Recombinant protein of human ATP-binding cassette, sub-family G (WHITE), member 1 (ABCG1), transcript variant 2  
ABCG1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

4 Item(s)

per page

Available Filters

Product Type
  1. Primary Antibody (3)
  2. Protein | Growth Factor (1)
Clonality
  1. Polyclonal (3)
Target
  1. ABCG1 (4)
Reactivity
  1. Human (Hu) (3)
  2. Mouse (Ms) (2)
  3. Rat (Rt) (2)
Applications
  1. Paraffin Sections (P) (3)
  2. Enzyme Immunoassay (E) (2)
  3. Western blot / Immunoblot (WB) (2)
  4. Flow cytometry (F) (1)
Presentation
  1. Purified (3)
Host
  1. Rabbit (3)
Images
  1. 1 image (4)
  2. 3+ images (1)
Images: Applications
  1. IHC (2)
  2. Western blot / Immunoblot (WB) (2)
  3. Flow cytometry (F) (1)
  4. Paraffin Sections (P) (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (4)
Service
  • OriGene Technologies GmbH
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  • Germany
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