ABHD12 antibody
Principal name
ABHD12 antibody
Alternative names for ABHD12 antibody
C20orf22, Abhydrolase domain-containing protein 12
Ncbi ID
9606, NP_001035937, NP_001035937.1, NP_056415.1, NM_015600, NM_001042472, NP_077785.2, NM_024465
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of ABHD12 antibody
- Kit Component:
- - KN300648G1, Abhd12 gRNA vector 1 in pCas-Guide vector
- KN300648G2, Abhd12 gRNA vector 2 in pCas-Guide vector
- KN300648D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Blankman JL, Simon GM, Cravatt BF.
A comprehensive profile of brain enzymes that hydrolyze the endocannabinoid 2-arachidonoylglycerol.
Chem Biol. 2007 Dec;14(12):1347-56
PMID: 18096503
Western Blot analysis of ABHD12 expression in transfected 293T cell line (H00026090-T01) by ABHD12 MaxPab polyclonal antibody.Lane 1: C20orf22 transfected lysate(44.44 KDa).Lane 2: Non-transfected lysate.
ABHD12 polyclonal antibody ( Cat # PAB11485 ) ( 0.3 µg/ ml ) staining of mouse brain lysate ( 35 µg/ protein in RIPA buffer ). Primary incubation was 1 hour. Detected by chemiluminescence.
Human Spleen; WB Suggested Anti-ABHD12 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Spleen; ABHD12 antibody - middle region (ARP44941_P050) in Human Spleen cells using Western Blot
TA305873 (0.3ug/ ml) staining of Mouse Brain lysate (35ug/ protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of THP1 cells (Lanes 1 and 2: 60ug lysate) with anti-ABHD12 antibody diluated at 1:2000. (Predicted and observed size: 45kDa)
Western blot analysis of extracts from COLO cells, using ABHD12 antibody.The lane on the right is treated with the synthesized peptide.
Predicted cell location: Cytoplasm. Positive control: Human thyroid and liver cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using ABHD12 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x 200)
Predicted cell location: Cytoplasm. Positive control: Human thyroid and liver cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using ABHD12 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x200)
Predicted cell location: Cytoplasm. Positive control: Human thyroid and liver cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using ABHD12 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x 200)
Predicted cell location: Cytoplasm. Positive control: Human thyroid and liver cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using ABHD12 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x200)
WB Suggested Anti-ABHD12 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Spleen
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA305873 | ABHD12 antibody |
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| Goat Anti-ABHD12 Antibody | |||||||
 |
Goat | Aff - Purified | Ms, Rt | E, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA310141 | ABHD12 antibody |
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| Rabbit anti-ABHD12 polyclonal antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€195.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA313208 | ABHD12 antibody |
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| Rabbit polyclonal anti-ABHD12 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA323295 | ABHD12 antibody |
||||||
| Anti-ABHD12 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
| TA323296 | ABHD12 antibody |
||||||
| Anti-ABHD12 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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