ACADM antibody
Principal name
ACADM antibody
Alternative names for ACADM antibody
MCAD, Medium-chain specific acyl-CoA dehydrogenase mitochondrial
Ncbi ID
9606, NP_000007, NP_000007.1, NM_001127328, NP_031408.1, NM_007382, NM_016986, NM_000016
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F), Immunoprecipitation (IP)
Background of ACADM antibody
- Kit Component:
- - KN300692G1, Acadm gRNA vector 1 in pCas-Guide vector
- KN300692G2, Acadm gRNA vector 2 in pCas-Guide vector
- KN300692D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Powelka et al, Suppression of oxidative metabolism and mitochondrial biogenesis by the transcriptional corepressor RIP140 in mouse adipocytes. J Clin Invest. 2006 Jan;116(1):125-36. Epub 2005 Dec 22.
PMID: 16374519
Human HepG2; WB Suggested Anti-ACADM Antibody Titration: 5.0ug/ml. Positive Control: HepG2 cell lysate; ACADM antibody - N-terminal region (ARP32789_T100) in Human HepG2 cells using Western Blot
Immunoperoxidase of purified MaxPab antibody to ACADM on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]
Immunofluorescence of purified MaxPab antibody to ACADM on HeLa cell. [antibody concentration 10 ug/ml]
ACADM MaxPab polyclonal antibody. Western Blot analysis of ACADM expression in human liver.
Western Blot analysis of ACADM expression in transfected 293T cell line (H00000034-T01) by ACADM MaxPab polyclonal antibody.Lane 1: ACADM transfected lysate(46.31 KDa).Lane 2: Non-transfected lysate.
ACADM MaxPab polyclonal antibody. Western Blot analysis of ACADM expression in HepG2.
ACADM polyclonal antibody ( Cat # PAB6264 ) ( 3.8µg/ml ) staining of paraffin embedded human liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ACADM polyclonal antibody ( Cat # PAB6264 ) staining ( 0.05 µg/ml ) of human heart lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Human 721_B; WB Suggested Anti-ACADM Antibody Titration: 0.2-1 ug/ml. Positive Control: 721_B cell lysate. ACADM is supported by BioGPS gene expression data to be expressed in 721_B.; ACADM antibody - N-terminal region (ARP32788_P050) in Human 721_B cells using Western Blot
Skeletal muscle: Formalin-Fixed Paraffin-Embedded (FFPE)
Immunoperoxidase of purified MaxPab antibody to ACADM on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]
Immunofluorescence of purified MaxPab antibody to ACADM on HeLa cell. [antibody concentration 10 ug/ml]
ACADM MaxPab polyclonal antibody. Western Blot analysis of ACADM expression in human liver.
Western Blot analysis of ACADM expression in transfected 293T cell line (H00000034-T01) by ACADM MaxPab polyclonal antibody.Lane 1: ACADM transfected lysate(46.31 KDa).Lane 2: Non-transfected lysate.
ACADM MaxPab polyclonal antibody. Western Blot analysis of ACADM expression in HepG2.
ACADM MaxPab rabbit polyclonal antibody. Western Blot analysis of ACADM expression in mouse kidney.
Western Blot analysis of ACADM expression in transfected 293T cell line (H00000034-T02) by ACADM MaxPab polyclonal antibody.Lane 1: ACADM transfected lysate(46.60 KDa).Lane 2: Non-transfected lysate.
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with ACADM Antibody (Center) Cat.-No AP17073PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated
Confocal immunofluorescent analysis of ACADM Antibody (Center) Cat.-No AP17073PU-N with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of ACADM Antibody (Center) Cat.-No AP17073PU-N in mouse heart tissue lysates (35 µg/lane). ACADM (arrow) was detected using the purified Pab.
Flow cytometric analysis of widr cells usingACADM Antibody (Center) Cat.-No AP17073PU-N (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ACADM MaxPab rabbit polyclonal antibody. Western Blot analysis of ACADM expression in mouse kidney.
Immunoprecipitation of ACADM transfected lysate using anti-ACADM MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ACADM MaxPab mouse polyclonal antibody (B01) (H00000034-B01).
Recombinant human ACADM, 26-421 aa, His-tagged
TA302400 staining (0.05µg/ml) of Human Heart lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
TA302400 (3.8µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
WB Suggested Anti-ACADM Antibody Titration: 0.2-1 ug/ml; Positive Control: 721_B cell lysateACADM is supported by BioGPS gene expression data to be expressed in 721_B
WB Suggested Anti-ACADM Antibody Titration: 5.0ug/ml; Positive Control: HepG2 cell lysate
Western blot analysis of extracts of HepG2 cell line, using ACADM antibody.
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP17073PU-N | ACADM antibody |
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 |
Rabbit | Ig | Purified | Hu, Ms | F, ICC/IF, P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA302400 | ACADM (C-term) antibody |
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| Goat Polyclonal Antibody against ACADM | |||||||
 |
Goat | Aff - Purified | Hu | P, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA332452 | ACADM antibody |
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| Rabbit anti-ACADM Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
| AP08218PU-N | ACADM antibody |
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 |
Rabbit | IgG | Purified | Hu, Ms, Por, Sh | P, WB |
0.1 ml /
€425.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP302798 | ACADM (transcript variant 1) |
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| Recombinant protein of human acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain (ACADM), nuclear gene encoding mitochondrial protein, transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY426749 | ACADM overexpression lysate |
|
 |
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR206704L2V | Lenti ORF particles, Acadm (GFP-tagged) - Mouse acyl-Coenzyme A dehydrogenase, medium chain (Acadm), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR206704L1V | Lenti ORF particles, Acadm (Myc-DDK-tagged) - Mouse acyl-Coenzyme A dehydrogenase, medium chain (Acadm), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR209147L2V | Lenti ORF particles, Acadm (GFP-tagged ORF) - Rat acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain (Acadm), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR209147L1V | Lenti ORF particles, Acadm (Myc-DDK-tagged ORF) - Rat acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain (Acadm), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
