ACADVL antibody
Principal name
ACADVL antibody
Alternative names for ACADVL antibody
VLCAD, Very long-chain specific acyl-CoA dehydrogenase mitochondrial
Ncbi ID
9606, NP_001029031, NP_000009, NP_000009.1, NM_001033859, NP_059062.1, NM_017366, NM_012891, NM_000018
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of ACADVL antibody
- Kit Component:
- - KN300695G1, Acadvl gRNA vector 1 in pCas-Guide vector
- KN300695G2, Acadvl gRNA vector 2 in pCas-Guide vector
- KN300695D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
1. Gobin-Limballe S, Djouadi F, Aubey F, Olpin S, Andresen BS, Yamaguchi S, Mandel H, Fukao T, Ruiter JP, Wanders RJ, McAndrew R, Kim JJ, Bastin J. Genetic basis for correction of very-long-chain acyl-coenzyme A dehydrogenase deficiency by bezafibrate in patient fibroblasts: toward a genotype-based therapy. Am J Hum Genet. 2007 Dec;81(6):1133-43.
PMID: 17999356
Immunoperoxidase of monoclonal antibody to ACADVL on formalin-fixed paraffin-embedded human heart. [antibody concentration 3 ug/ml]
ACADVL monoclonal antibody (M01), clone 5D3 Western Blot analysis of ACADVL expression in Hela NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged ACADVL is approximately 0.03ng/ml as a capture antibody.
ACADVL polyclonal antibody (A01), Lot # 051116JC01 Western Blot analysis of ACADVL expression in A-431 ( Cat # L015V1 ).
Immunofluorescence of purified MaxPab antibody to ACADVL on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of ACADVL expression in transfected 293T cell line (H00000037-T01) by ACADVL MaxPab polyclonal antibody.Lane 1: ACADVL transfected lysate(72.05 KDa).Lane 2: Non-transfected lysate.
Human Pineal Tissue; Rabbit Anti-ACADVL Antibody . Catalog Number: ARP54486_P050 . Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue . Observed Staining: Cytoplasmic in cell bodies of pinealocytes . Primary Antibody Concentration: 1:100 . Secondary Antibody: Donkey anti-Rabbit-Cy3 . Secondary Antibody Concentration: 1:200 . Magnification: 20X . Exposure Time: 0.5 - 2.0 sec .; Rabbit Anti-ACADVL Antibody (ARP54486_P050) ) in Human Pineal Tissue using Immunohistochemistry
Human Hela; Host: Rabbit. Target Name: EGFL8 . Sample Tissue: Hela . Antibody Dilution: 1.0ug/ml. ACADVL is supported by BioGPS gene expression data to be expressed in HeLa.; ACADVL antibody - N-terminal region (ARP54486_P050) in Human Hela cells using Western Blot
Hum. Fetal Muscle; Host: Rabbit. Target Name: NOP56 . Sample Tissue: Human Fetal Muscle . Antibody Dilution: 1.0ug/ml.; ACADVL antibody - N-terminal region (ARP54486_P050) in Hum. Fetal Muscle cells using Western Blot
Recombinant protein; . Lanes: 1: Normal controls Normal enzyme expression . 2: Normal controls Normal enzyme expression . 3: Positive mutants Defective enzyme expression . 4: Positive mutants Defective enzyme expression . 5: Positive mutants Defective enzyme expression. Primary Antibody Dilution: 1:2000. Secondary Antibody: . Secondary Antibody Dilution: 1:1000. Gene Name: ACADVL. Submitted by: David Lombert, Medical College of Wisconsin.; ACADVL antibody - N-terminal region (ARP54486_P050) in Recombinant protein cells using Western Blot
Human HepG2; WB Suggested Anti-ACADVL Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate. ACADVL is supported by BioGPS gene expression data to be expressed in HepG2.; ACADVL antibody - N-terminal region (ARP54486_P050) in Human HepG2 cells using Western Blot
Hum. Adult Placenta; Host: Rabbit. Target Name: SERPINA3 . Sample Tissue: Human Adult Placenta . Antibody Dilution: 1.0ug/ml.; ACADVL antibody - N-terminal region (ARP54486_P050) in Hum. Adult Placenta cells using Western Blot
Recombinant human ACADVL, 41-655 aa, His-tagged
Recombinant protein; . Lanes: 1: Normal controls Normal enzyme expression . 2: Normal controls Normal enzyme expression . 3: Positive mutants Defective enzyme expression . 4: Positive mutants Defective enzyme expression . 5: Positive mutants Defective enzyme expression. Primary Antibody Dilution: 1:2000. Secondary Antibody: . Secondary Antibody Dilution: 1:1000. Gene Name: ACADVL. Submitted by: David Lombert, Medical College of Wisconsin.; ACADVL antibody - C-terminal region (ARP54487_P050) in Recombinant protein cells using Western Blot
Human ACHN; WB Suggested Anti-ACADVL Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: ACHN cell lysate. ACADVL is supported by BioGPS gene expression data to be expressed in ACHN.; ACADVL antibody - C-terminal region (ARP54487_P050) in Human ACHN cells using Western Blot
AP32801PU-N ACADVL Antibody staining of Human Heart lysate at 1 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
(2ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of ACADVL with rat kidney tissue lysate.
Lanes: 1: Normal controls Normal enzyme expression; 2: Normal controls Normal enzyme expression; 3: Positive mutants Defective enzyme expression; 4: Positive mutants Defective enzyme expression; 5: Positive mutants Defective enzyme expression; Primary Ant
2Hum. Fetal Muscle; Host: Rabbit; Target Name: NOP56; Sample Tissue: Human Fetal Muscle; Antibody Dilution: 1.0 ug/ml
3Hum. Adult Placenta; Host: Rabbit; Target Name: SERPINA3; Sample Tissue: Human Adult Placenta; Antibody Dilution: 1.0 ug/ml
WB Suggested Anti-ACADVL Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysateACADVL is supported by BioGPS gene expression data to be expressed in HepG2
5Human Hela; Host: Rabbit; Target Name: EGFL8; Sample Tissue: Hela; Antibody Dilution: 1.0 ug/mlACADVL is supported by BioGPS gene expression data to be expressed in HeLa
Rabbit Anti-ACADVL Antibody; Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue; Observed Staining: Cytoplasmic in cell bodies of pinealocytes; Primary Antibody Concentration: 1: 100; Secondary Antibody: Donkey anti-Rabbit-Cy3; Secondary Antibod
Lanes: 1: Normal controls Normal enzyme expression; 2: Normal controls Normal enzyme expression; 3: Positive mutants Defective enzyme expression; 4: Positive mutants Defective enzyme expression; 5: Positive mutants Defective enzyme expression; Primary Ant
WB Suggested Anti-ACADVL Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: ACHN cell lysateACADVL is supported by BioGPS gene expression data to be expressed in ACHN
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP32801PU-N | ACADVL (C-term) antibody |
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Goat | Aff - Purified | Bov, Can, Hu | E, WB |
0.1 mg /
€380.00
|
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| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Western blotting. |
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| TA311114 | ACADVL antibody |
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| Goat Anti-ACADVL (VLCAD) Antibody | |||||||
 |
Goat | Aff - Purified | Hu | E, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA318885 | ACADVL antibody |
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| Rabbit polyclonal anti-ACADVL antibody | |||||||
 |
Rabbit | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€355.00
|
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| OriGene Technologies, Inc. | |||||||
| TA350728 | ACADVL antibody |
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| Rabbit Polyclonal Anti-ACADVL Antibody | |||||||
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Rabbit | IgG | Purified | Hu | E, P, WB |
0.1 ml /
€335.00
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|
| OriGene Technologies, Inc. | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN204428 | ACADVL - human gene knockout kit via CRISPR |
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Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN204428BN | ACADVL - human gene knockout kit via CRISPR |
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|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN204428LP | ACADVL - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN204428RB | ACADVL - human gene knockout kit via CRISPR |
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|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP305849 | ACADVL (transcript variant 2) |
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| Recombinant protein of human acyl-Coenzyme A dehydrogenase, very long chain (ACADVL), nuclear gene encoding mitochondrial protein, transcript variant 2 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY422424 | ACADVL overexpression lysate |
|
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0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR209811L2V | Lenti ORF particles, Acadvl (GFP-tagged) - Mouse acyl-Coenzyme A dehydrogenase, very long chain (Acadvl), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
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200 µl /
€1,410.00
|
|
| OriGene Technologies, Inc. | ||
| MR209811L1V | Lenti ORF particles, Acadvl (Myc-DDK-tagged) - Mouse acyl-Coenzyme A dehydrogenase, very long chain (Acadvl), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
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|
200 µl /
€1,260.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR203012L1V | Lenti ORF particles, Acadvl (Myc-DDK-tagged ORF) - Rat acyl-Coenzyme A dehydrogenase, very long chain (Acadvl), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
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|
200 µl /
€1,260.00
|
|
| OriGene Technologies, Inc. | ||