ACO2 antibody
Principal name
ACO2 antibody
Alternative names for ACO2 antibody
Aconitase 2, Aconitate hydratase mitochondrial
SwissProt ID
Q99798 (Human)
Gene ID
50 (ACO2)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)
Background of ACO2 antibody
ACO2 belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification.The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
General readings
1. Bota DA, Davies KJ. Lon protease preferentially degrades oxidized mitochondrial aconitase by an ATP-stimulated mechanism. Nat Cell Biol. 2002 Sep;4(9):674-80.
PMID: 12198491
Perfused isolated rat heart whole tissue lysate was lysed with either A ) 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.5% Na-deoxycholate, 1 mM Na3VO4 , 20 mM NaF, 1 mM PMSF, 5 v/v % protease inhibitor cocktail or B ) T-PER Tissue Protein Extraction Reagent ( Pierce ) , containing 1mM Na3VO4 , 20 mM NaF, 5 v/v % protease inhibitor cocktail ( Sigma ) ; PVDF membrane was incubated in primary Ab ACO2 polyclonal antibody ( Cat # PAB3751 ) . Solution : 1 : 1000 diluted in 5% NFM TBS-T 0,05 for overnight ( 15 hrs ) at 4 °C. Data courtesy of Boglarka Laczy M.D., Division of Cardiovascular Disease, Dept. of Medicine, University of Alabama at Birmingham.
Western blot analysis of ACO2 polyclonal antibody ( Cat # PAB3751 ) in mouse heart ( left ) and 293 ( right ) cell lysates ( 35 µg/lane ) . ACO2 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
Western blot analysis of anti-ACO2 (AP11745PU-N) in mouse heart (left) and 293 (right) tissue lysates (35 µg/lane). ACO2 (arrow) was detected using the purified Pab (1:60 dilution).
Formalin-fixed and paraffin-embedded human Heart tissue reacted with ACO2 Antibody (Center)(AP11745PU-N), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Perfused isolated rat heart whole tissue lysate was lysed with either A) 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.5% Na-deoxycholate, 1 mM Na3VO4, 20 mM NaF, 1 mM PMSF, 5 v/v % protease inhibitor cocktail or B) T-PER Tissue Protein Extraction Reagent, containing 1mM Na3VO4, 20 mM NaF, 5 v/v % protease inhibitor cocktail; PVDF membrane was incubated in primary Ab [rabbit polyclonal antibody against ACO2 (Center) (AP11745PU-N). Solution: 1:1000 diluted in 5% NFM TBS-T 0,05 for overnight (15 hrs) at 4 ºC. Data courtesy of Boglarka Laczy M.D., Division of Cardiovascular Disease, Dept. of Medicine, University of Alabama at Birmingham.
Western Blot analysis of ACO2 expression in transfected 293T cell line (H00000050-T02) by ACO2 MaxPab polyclonal antibody.Lane 1: ACO2 transfected lysate(85.40 KDa).Lane 2: Non-transfected lysate.
Human Pineal Tissue; Rabbit Anti-ACO2 Antibody . Catalog Number: ARP56302_P050 . Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue . Observed Staining: Cytoplasmic in cell bodies of penealocytes and connective tissue (septum . Primary Antibody Concentration: 1:100 . Secondary Antibody: Donkey anti-Rabbit-Cy3 . Secondary Antibody Concentration: 1:200 . Magnification: 20X . Exposure Time: 0.5 - 2.0 sec .; Rabbit Anti-ACO2 Antibody (ARP56302_P050) ) in Human Pineal Tissue using Immunohistochemistry
Human 721_B; Host: Rabbit. Target Name: WT1 . Sample Tissue: 721_B . Antibody Dilution: 1.0ug/ml. ACO2 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; ACO2 antibody - C-terminal region (ARP56302_P050) in Human 721_B cells using Western Blot
Human Heart; WB Suggested Anti-ACO2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human heart; ACO2 antibody - C-terminal region (ARP56302_P050) in Human Heart cells using Western Blot
Hum. Fetal Brain; Host: Rabbit. Target Name: NOP56 . Sample Tissue: Human Fetal Brain . Antibody Dilution: 1.0ug/ml.; ACO2 antibody - C-terminal region (ARP56302_P050) in Hum. Fetal Brain cells using Western Blot
Human 721_B; WB Suggested Anti-ACO2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 721_B cell lysate. ACO2 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; ACO2 antibody - N-terminal region (ARP56301_P050) in Human 721_B cells using Western Blot
Rat kidney; ACO2 antibody - N-terminal region (ARP56301_P050) validated by WB using Proximal kidney tubules purfied from cortex at 5.0ug/ml.; ACO2 antibody - N-terminal region (ARP56301_P050) in Rat kidney cells using Western Blot
Human Heart (formalin-fixed, paraffin-embedded) stained with ACO2 antibody AP22773PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Skeletal Muscle (formalin-fixed, paraffin-embedded) stained with ACO2 antibody AP22773PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot analysis of mouse heart(left) and 293 (right) tissue lysates (35 µg/lane).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-ACO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500824, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-ACO2mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500824, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-ACO2mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500824, Dilution 1:50)
Anti-ACO2 mouse monoclonal antibody (TA500824) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACO2(RC204307).
Immunofluorescent staining of HepG2 cells using anti-ACO2 mouse monoclonal antibody (TA500824).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACO2 (RC204307, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACO2.
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-ACO2 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Anti-ACO2 mouse monoclonal antibody (TA500841) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACO2(RC204307).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACO2 (RC204307, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACO2.
HEK293T cells transfected with either pCMV6-ENTRY ACO2(RC204307)(Red) or empty vector control plasmid(Blue) were immunostained with anti-ACO2 mouse monoclonal(TA500841), and then analyzed by flow cytometry.
Flow cytometric analysis of Jurkat cells, using anti-ACO2 antibody(TA500841),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Flow cytometric analysis of Hela cells, using anti-ACO2 antibody(TA500841),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Immunofluorescent staining of HepG2 cells using anti-ACO2 mouse monoclonal antibody (TA500873).
Anti-ACO2 mouse monoclonal antibody (TA500873) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACO2(RC204307).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACO2 (RC204307, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACO2.
Western blot analysis of extracts (10ug) from 7 different cell lines by using anti-ACO2 monoclonal antibody.(1:200)
HEK293T cells transfected with either pCMV6-ENTRY ACO2(RC204307)(Red) or empty vector control plasmid(Blue) were immunostained with anti-ACO2 mouse monoclonal(TA500873), and then analyzed by flow cytometry.
Anti-ACO2 mouse monoclonal antibody (TA500913) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACO2(RC204307).
HEK293T cells transfected with either pCMV6-ENTRY ACO2(RC204307)(Red) or empty vector control plasmid(Blue) were immunostained with anti-ACO2 mouse monoclonal(TA500913), and then analyzed by flow cytometry.
Western blot analysis of ACO2 (arrow) using rabbit polyclonal ACO2 Antibody (Center) (Cat.#TA302090).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACO2 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ACO2 antibody (Center) (Cat.#TA302090), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
(0.01ug/ml) staining of Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Predicted band size: 85 kDa. Positive control: Mouse heart tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 seconds)
Predicted cell location: Cytoplasm. Positive control: Human lung cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer using ACO2 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification: x 200)
Predicted band size: 85 kDa. Positive control: Mouse heart tissue and hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: Mouse heart tissue lysate Lane2: Hela cell lysate Lysates: 40 ug per lane Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 3 seconds)
Predicted cell location: Cytoplasm, Nucleus. Positive control: Human lung cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer using ACO2 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification: x 200)
TA349000 (0.01 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA349000 (0.1 ug/ml) staining of Human (A), Mouse (B) and Rat (C) Adipose lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA349000 (0.01 ug/ml) staining of Mouse (A), Rat (B) and Pig (C) Skeletal Muscle lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA349000 (3.8 ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP11745PU-N | ACO2 antibody |
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Rabbit | Ig | Purified | Hu, Ms, Rt | P, WB |
0.4 ml /
€415.00
|
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| OriGene Technologies GmbH | |||||||
 +2 additional images |
Published recently! |
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| TA349000 | ACO2 (541-555) antibody |
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| Goat Polyclonal Anti-Aconitase 2 (aa541-555) Antibody | |||||||
 |
Goat | Aff - Purified | Bov, Can, Hu, Ms, Por, Rt | E, P, WB |
0.1 mg /
€360.00
|
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| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody! |
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| TA500824 | ACO2 antibody |
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| anti-ACO2 (Aconitase 2) mouse monoclonal antibody, clone OTI3G8 (formerly 3G8) | |||||||
 |
Mouse | IgG1 | OTI3G8 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
|
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| TA500841 | ACO2 antibody |
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| anti-ACO2 (Aconitase 2) mouse monoclonal antibody, clone OTI7A11 (formerly 7A11) | |||||||
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Mouse | IgG1 | OTI7A11 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA500873 | ACO2 antibody |
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| anti-ACO2 (Aconitase 2) mouse monoclonal antibody, clone OTI7G4 (formerly 7G4) | |||||||
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Mouse | IgG1 | OTI7G4 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA302090 | ACO2 antibody |
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| Rabbit Polyclonal Antibody against ACO2 (Center) | |||||||
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Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA311180 | ACO2 antibody |
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| Goat Anti-Aconitase 2 / ACO2 Antibody | |||||||
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Goat | Aff - Purified | Bov, Can, Hu, Ms, Por, Rt | E, ICC/IF, P, WB |
0.1 mg /
€360.00
|
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| OriGene Technologies, Inc. | |||||||
| TA321275 | ACO2 antibody |
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| Anti-ACO2 rabbit polyclonal antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA321276 | ACO2 antibody |
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| Anti-ACO2 rabbit polyclonal antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA500913 | ACO2 antibody |
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| anti-ACO2 (Aconitase 2) mouse monoclonal antibody, clone OTI3G10 (formerly 3G10) | |||||||
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Mouse | IgG2a | OTI3G10 | Purified | Hu | F, ICC/IF |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP304307 | ACO2 |
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| Recombinant protein of human aconitase 2, mitochondrial (ACO2), nuclear gene encoding mitochondrial protein | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
