ACOT12 antibody
Principal name
ACOT12 antibody
Alternative names for ACOT12 antibody
Acyl-CoA thioesterase 12, CACH, CACH1, STARD12, Acyl-coenzyme A thioesterase 12, Acyl-CoA thioester hydrolase 12, Cytoplasmic acetyl-CoA hydrolase 1, START domain-containing protein 12
Ncbi ID
NP_570123, NP_570123.1, NM_130767, NP_083066.1, NM_028790, NM_130747
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F)
Background of ACOT12 antibody
- Kit Component:
- - KN300730G1, Acot12 gRNA vector 1 in pCas-Guide vector
- KN300730G2, Acot12 gRNA vector 2 in pCas-Guide vector
- KN300730D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Hunt,M.C., (2006) FASEB J. 20 (11), 1855-1864
Human 721_B; WB Suggested Anti-ACOT12 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: 721_B cell lysate; ACOT12 antibody - middle region (ARP52535_P050) in Human 721_B cells using Western Blot
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ACOT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502396)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ACOT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502396)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ACOT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502396)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ACOT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502396)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ACOT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502396)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ACOT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502396)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACOT12 (RC210445, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACOT12.
HEK293T cells transfected with either RC210445 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ACOT12 antibody(TA502396), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-ACOT12 antibody(TA502396),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ACOT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502462)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ACOT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502462)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ACOT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502462)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ACOT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502462)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ACOT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502462)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ACOT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502462)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-ACOT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502462)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACOT12 (RC210445, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACOT12.
Flow cytometric Analysis of Hela cells, using anti-ACOT12 antibody(TA502462),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells transfected with either RC210445 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ACOT12 antibody(TA502462), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACOT12 (RC210445, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACOT12.
HEK293T cells transfected with either RC210445 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ACOT12 antibody(TA502477), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACOT12 (RC210445, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACOT12.
HEK293T cells transfected with either RC210445 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ACOT12 antibody(TA502530), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-ACOT12 antibody(TA502530),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Hela cells, using anti-ACOT12 antibody(TA502530),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACOT12 (RC210445, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACOT12.
HEK293T cells transfected with either RC210445 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ACOT12 antibody(TA502686), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-ACOT12 antibody(TA502686),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Hela cells, using anti-ACOT12 antibody(TA502686),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using ACOT12 antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of A549 cells, using ACOT12 antibody.The picture on the right is treated with the synthesized peptide.
WB Suggested Anti-ACOT12 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: 721_B cell lysate
- Quick-Filters:
- Highly Validated (4)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA502396 | ACOT12 antibody |
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| ACOT12 mouse monoclonal antibody, clone OTI1A11 (formerly 1A11) | |||||||
 |
Mouse | IgG2a | OTI1A11 | Purified | Hu | F, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +8 additional images |
Highly validated antibody!
|
||||||
| TA502462 | ACOT12 antibody |
||||||
| ACOT12 mouse monoclonal antibody, clone OTI1B9 (formerly 1B9) | |||||||
 |
Mouse | IgG2a | OTI1B9 | Purified | Hu, Mky | F, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +10 additional images |
Highly validated antibody!
|
||||||
| TA502530 | ACOT12 antibody |
||||||
| ACOT12 mouse monoclonal antibody, clone OTI1D3 (formerly 1D3) | |||||||
 |
Mouse | IgG2a | OTI1D3 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
||||||
| TA502686 | ACOT12 antibody |
||||||
| ACOT12 mouse monoclonal antibody, clone OTI2F10 (formerly 2F10) | |||||||
 |
Mouse | IgG2a | OTI2F10 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
||||||
| TA312609 | ACOT12 antibody |
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| Rabbit polyclonal anti-ACOT12 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, ICC/IF, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA502477 | ACOT12 antibody |
||||||
| ACOT12 mouse monoclonal antibody, clone OTI2D11 (formerly 2D11) | |||||||
 |
Mouse | IgG2a | OTI2D11 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP310445 | ACOT12 |
||||
| Recombinant protein of human acyl-CoA thioesterase 12 (ACOT12) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY408930 | ACOT12 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR214768L2V | Lenti ORF particles, Acot12 (GFP-tagged ORF) - Rat acyl-CoA thioesterase 12 (Acot12), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,260.00
|
|
| OriGene Technologies, Inc. | ||
| RR214768L1V | Lenti ORF particles, Acot12 (Myc-DDK-tagged ORF) - Rat acyl-CoA thioesterase 12 (Acot12), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,130.00
|
|
| OriGene Technologies, Inc. | ||