ACOT2 antibody
Principal name
ACOT2 antibody
Alternative names for ACOT2 antibody
PTE2, PTE2A, Acyl-CoA thioesterase 2, ZAP128, CTE-Ia, Acyl-coenzyme A thioester hydrolase 2a, Long-chain acyl-CoA thioesterase 2, Acyl-coenzyme A thioesterase 2
Gene ID
10965 (ACOT2)
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of ACOT2 antibody
- Kit Component:
- - KN202837G1, ACOT2 gRNA vector 1 in pCas-Guide vector
- KN202837G2, ACOT2 gRNA vector 2 in pCas-Guide vector
- KN202837D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
"Identification of PTE2, a human peroxisomal long-chain acyl-CoA thioesterase." Jones J.M., Gould S.J. Biochem. Biophys. Res. Commun. 275:233-240(2000)
Hum. Fetal Liver; Host:Rabbit. Target Name:ACOT2. Sample Tissue:Human Fetal Liver. Antibody Dilution: 1.0ug/ml.; ACOT2 antibody - middle region (ARP42322_P050) in Hum. Fetal Liver cells using Western Blot
Human Heart; WB Suggested Anti-ACOT2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human heart; ACOT2 antibody - middle region (ARP42322_P050) in Human Heart cells using Western Blot
Hum. Fetal Liver; Host:Rabbit. Target Name:ACOT2. Sample Tissue:Human Fetal Liver. Antibody Dilution: 1.0ug/ml.; ACOT2 antibody - middle region (AP43237PU-N) in Hum. Fetal Liver cells using Western Blot
Human Heart; WB Suggested Anti-ACOT2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human heart; ACOT2 antibody - middle region (AP43237PU-N) in Human Heart cells using Western Blot
Immunohistochemical analysis of ACOT2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of ACOT2 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of ACOT2 expression in K562 (A); HT29 (B) whole cell lysates.
Western blot analysis of extracts from Jurkat cells and HuvEc cells, using ACOT2 antibody.The lane on the right is treated with the synthesized peptide.
Predicted band size: 53 kDa . Positive control: Mouse kidney and liver tissue lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lane1: Mouse kidney tissue lysate Lane2: Mouse liver tissue lysate Lysates: 40 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 minutes)
Predicted cell location: Cytoplasm. Positive control: Human lung cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer using ACOT2 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification: x 200)
Predicted band size: 53 kDa . Positive control: MCF7 cell lysate. Recommended dilution: 1/100-500. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/150 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 minutes)
Predicted cell location: Cytoplasm. Positive control: Human lung cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer using ACOT2 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification: x 200)
WB Suggested Anti-ACOT2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human heart
Host: Rabbit; Target Name: ACOT2; Sample Tissue: Human Fetal Liver; Antibody Dilution: 1.0 ug/ml
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA315131 | ACOT2 antibody |
||||||
| Rabbit polyclonal anti-ACOT2 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA322465 | ACOT2 antibody |
||||||
| Anti-ACOT2 rabbit polyclonal antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
| TA322466 | ACOT2 antibody |
||||||
| Anti-ACOT2 rabbit polyclonal antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
