ACOT8 antibody
Principal name
ACOT8 antibody
Alternative names for ACOT8 antibody
ACTEIII, PTE1, PTE2, Acyl-CoA thioesterase 8, PTE-2, Choloyl-coenzyme A thioesterase, Peroxisomal acyl-coenzyme A thioester hydrolase 1, Peroxisomal long-chain acyl-CoA thioesterase 1, HIV-Nef-associated acyl-CoA thioesterase, Thioesterase II
Ncbi ID
9606, NP_005460.2, NM_005469, NP_005460, NP_573503.2, NM_133240, NM_183386
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Flow Cytometry (F)
Background of ACOT8 antibody
- Kit Component:
- - KN300738G1, Acot8 gRNA vector 1 in pCas-Guide vector
- KN300738G2, Acot8 gRNA vector 2 in pCas-Guide vector
- KN300738D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Ishizuka M., et al. (2004) Exp. Cell Res. 297:127-141Hunt M.C., et al. (2002) Prog. Lipid Res. 41:99-130
Immunofluorescence of purified MaxPab antibody to ACOT8 on HeLa cell. [antibody concentration 10 ug/ml]
ACOT8 MaxPab polyclonal antibody. Western Blot analysis of ACOT8 expression in human spleen.
Western Blot analysis of ACOT8 expression in transfected 293T cell line (H00010005-T01) by ACOT8 MaxPab polyclonal antibody.Lane 1: ACOT8 transfected lysate(35.09 KDa).Lane 2: Non-transfected lysate.
ACOT8 monoclonal antibody (M03), clone 3F1. Western Blot analysis of ACOT8 expression in A-431(Cat # L015V1 ).
Human 721_B; WB Suggested Anti-ACOT8 Antibody. . Titration: 1.0 ug/ml. . Positive Control: 721_B Whole Cell. ACOT8 is supported by BioGPS gene expression data to be expressed in 721_B.; ACOT8 antibody - N-terminal region (ARP62050_P050) in Human 721_B cells using Western Blot
Formalin-fixed and paraffin-embedded human Prostate carcinoma reacted with ACOT8 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of ACOT8 Antibody (C-term) (Cat. #AP50053PU-N) in mouse liver tissue lysates (35 µg/lane). ACOT8 (arrow) was detected using the purified Pab.
ACOT8 Antibody (C-term) (Cat. #AP50053PU-N) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunohistochemical analysis of ACOT8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of ACOT8 staining in Raji cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of ACOT8 expression in Raji (A); NIH3T3 (B); PC12 (C) whole cell lysates.
Western blot analysis of extracts from HUVEC cells, using ACOT8 antibody.The lane on the right is treated with the synthesized peptide.
Immunofluorescence analysis of A549 cells, using ACOT8 antibody.The picture on the right is treated with the synthesized peptide.
Predicted cell location: Cytoplasm. Positive control: Human cervical and thyroid cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human cervical cancer tissue using ACOT8 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x 200)
Predicted cell location: Cytoplasm. Positive control: Human cervical and thyroid cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using ACOT8 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x200)
Predicted cell location: Cytoplasm. Positive control: Human cervical and lung cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human cervical cancer tissue using ACOT8 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification:x 200)
Predicted cell location: Cytoplasm. Positive control: Human cervical and lung cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using ACOT8 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification:x200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP50053PU-N | ACOT8 (C-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu, Ms | F, P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
 +2 additional images |
|||||||
