ACOT9 antibody
Principal name
ACOT9 antibody
Alternative names for ACOT9 antibody
CGI-16, Acyl-CoA thioesterase 9, Acyl-coenzyme A thioesterase 9 mitochondrial
Ncbi ID
9606, NP_001028755, NM_001037171, NP_001032248, NP_001028755.2, NP_062710.2, NM_019736, NM_001033583
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))
Background of ACOT9 antibody
- Kit Component:
- - KN300739G1, Acot9 gRNA vector 1 in pCas-Guide vector
- KN300739G2, Acot9 gRNA vector 2 in pCas-Guide vector
- KN300739D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunoperoxidase of monoclonal antibody to ACOT9 on formalin-fixed paraffin-embedded human heart. [antibody concentration 3ug/ml]
Immunofluorescence of monoclonal antibody to ACOT9 on HeLa cell. [antibody concentration 10 ug/ml]
ACATE2 monoclonal antibody (M01), clone 4E4 Western Blot analysis of ACATE2 expression in MCF-7 ( Cat # L046V1 ).
Western Blot analysis of ACOT9 expression in transfected 293T cell line by ACATE2 monoclonal antibody (M01), clone 4E4.Lane 1: ACOT9 transfected lysate(24 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged ACOT9 is approximately 3ng/ml as a capture antibody.
Western Blot analysis of ACOT9 expression in transfected 293T cell line (H00023597-T01) by ACOT9 MaxPab polyclonal antibody.Lane 1: ACOT9 transfected lysate(23.32 KDa).Lane 2: Non-transfected lysate.
Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence of monoclonal antibody to ACOT9 on HeLa cell (antibody concentration 10 µg/ml).
Western Blot analysis of ACOT9 expression in transfected 293T cell line by ACATE2 monoclonal antibody, clone 4E4.
ACATE2 monoclonal antibody, clone 4E4 Western Blot analysis of ACATE2 expression in MCF-7.
Detection limit for recombinant GST tagged ACOT9 is approximately 3 ng/ml as a capture antibody.
Human MDA-MB-435S; WB Suggested Anti-ACOT9 Antibody. . Titration: 1.0 ug/ml. . Positive Control: MDA-MB-435S Whole Cell; ACOT9 antibody - C-terminal region (ARP60816_P050) in Human MDA-MB-435S cells using Western Blot
Human Fetal Lung; WB Suggested Anti-ACOT9 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Fetal Lung; ACOT9 antibody - N-terminal region (ARP60817_P050) in Human Fetal Lung cells using Western Blot
Predicted band size: 50 kDa . Positive control: MCF7 and A172 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: MCF7 cell lysate Lane2: A172 cell lysate Lysates: 40 ug per lane Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 seconds)
Predicted cell location: Cytoplasm. Positive control: Human colon cancer tissue. Recommended dilution: 1/10-50 The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using ACOT9 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification: x 200)
Predicted band size: 50 kDa . Positive control: Human brain malignant glioma and human fetal kidney tissue, A172 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: Human brain malignant glioma tissue lysate Lane2 Human fetal kidney tissue lysate Lane3: A172 cell lysate Lysates: 40 ug per lane Primary antibody: 1/600 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 seconds)
WB Suggested Anti-ACOT9 Antibody; Titration: 1.0 ug/ml; Positive Control: MDA-MB-435S Whole Cell
WB Suggested Anti-ACOT9 Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Lung
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM20995PU-N | ACOT9 antibody |
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Mouse | IgG1 | 4E4 | Hu | E, ICC/IF, P, WB |
50 µg /
€475.00
|
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| OriGene Technologies GmbH | |||||||
 +5 additional images |
Highly validated antibody! |
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| TA323401 | ACOT9 antibody |
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| Anti-ACOT9 rabbit polyclonal antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA323402 | ACOT9 antibody |
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| Anti-ACOT9 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP316986 | ACOT9 (transcript variant 1) |
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| Recombinant protein of human acyl-CoA thioesterase 9 (ACOT9), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY421928 | ACOT9 overexpression lysate |
|
 |
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR206995L2V | Lenti ORF particles, Acot9 (GFP-tagged) - Mouse acyl-CoA thioesterase 9 (Acot9), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,100.00
|
|
| OriGene Technologies, Inc. | ||
| MR206995L1V | Lenti ORF particles, Acot9 (Myc-DDK-tagged) - Mouse acyl-CoA thioesterase 9 (Acot9), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€990.00
|
|
| OriGene Technologies, Inc. | ||