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ACTR3 antibody

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Principal name

ACTR3 antibody

Alternative names for ACTR3 antibody

ARP3, Actin-related protein 3, Actin-like protein 3

SwissProt ID

P61157 (Bovin), P61158 (Human), Q4V7C7 (Rat), Q90WD0 (Chick), Q99JY9 (Mouse)

Gene ID

10096 (ACTR3), 74117 (Actr3), 81732

Ncbi ID

9606, NP_005712, NP_005712.1, NM_023735, NM_031068, NM_005721, NP_001192314.1

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Rb (Rabbit), Ze (Zebrafish), Eq (Equine), GP (Guinea Pig)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of ACTR3 antibody

Actin nucleation, the formation of new actin filaments from existing filaments, affects actin filament structure during cell motility, division, and intracellular trafficking. An important actin nucleation protein complex is the highly conserved ARP2/3 complex, consisting of ARP2, ARP3, and ARPC1-5. The ARP2/3 complex promotes branching of an existing actin filament and formation of a daughter filament following activation by nucleation-promoting factors, such as WASP/WAVE or cortactin. The formation of podosomes, small cellular projections that degrade the extracellular matrix, is enhanced by ARP2/3 complex action. ARP2/3 competes with caldesmon, an actin binding protein shown to negatively affect podosome formation). Along with N-WASP, the ARP2/3 complex regulates nuclear actin filament nucleation and controls actin polymerization during transcription.

General readings

Welch M.D., et al. (1997) Nature. 385:265-269Kim J., et al. (2010) Nature. 464:1048-1051

H00010096-M02 - ACTR3
Immunoperoxidase of monoclonal antibody to ACTR3 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 6ug/ml]
H00010096-M02 - ACTR3
Immunofluorescence of monoclonal antibody to ACTR3 on HeLa cell. [antibody concentration 10 ug/ml]
H00010096-M02 - ACTR3
ACTR3 monoclonal antibody (M02), clone 2B6 Western Blot analysis of ACTR3 expression in A-431 ( Cat # L015V1 ).
AP17090PU-N - ACTR3
Western blot analysis of ACTR3 Antibody (C-term) (AP17090PU-N) in A2058 cell line lysates (35 µg/lane). ACTR3 (arrow) was detected using the purified Pab.
ARP52094_P050 - ACTR3
Human Lung; WB Suggested Anti-ACTR3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Lung; ACTR3 antibody - N-terminal region (ARP52094_P050) in Human Lung cells using Western Blot
C14244-1 - ACTR3
Western blot analysis of extracts from Jurkat/RAW264.7 cells, using ACTR3 Antibody. The lane on the right is treated with the synthesized peptide.
AR51655PU-N - ACTR3
CPA2453-100ul - ACTR3
Western blot analysis of ARP3 expression in A549 (A); Jurkat (B); HeLa (C); A431 (D) whole cell lysates.
TP308460 - ACTR3
TA315015 - ACTR3
Western blot analysis of extracts from RAW264.7 cells and Jurkat cells, using ACTR3 antibody.The lane on the right is treated with the synthesized peptide.
TA324284 - ACTR3
Predicted band size: 47 kDa . Positive control: k562, HL60, Hela, Jurkat, Cos1 and Cos7 cell, mouse spleen and liver tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: k562 cell lysate Lane2:HL60 cell ysate Lane3:Hela cell lysate Lane4:Jurkat cell lysate Lane5: Cos1 cell lysate Lane6: Cos7 cell lysate Lane7:Mouse spleen tissue lysate Lane8:Mouse liver tissue lysate; Primary antibody: 1/500; Secondary antibody: Goat anti Rabbit IgG - H&L (HRP): 1/10000)
TA326908 - ACTR3
Western blot analysis of extracts of various cell lines, using ACTR3 antibody.
TA326908 - ACTR3
Immunohistochemistry of paraffin-embedded human lung using ACTR3 antibody at dilution of 1:200 (400x lens).
TA326908 - ACTR3
Immunohistochemistry of paraffin-embedded human stomach cancer using ACTR3 antibody at dilution of 1:200 (400x lens).
TA340070 - ACTR3
WB Suggested Anti-ACTR3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human Lung

9 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ACTR3 antibody
  Rabbit polyclonal anti-ACTR3 antibody  
Western blot analysis of extracts from RAW264.7 cells and Jurkat cells, using ACTR3 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

ACTR3 antibody
  Anti-ACTR3 Rabbit Polyclonal Antibody  
Predicted band size: 47 kDa . Positive control: k562, HL60, Hela, Jurkat, Cos1 and Cos7 cell, mouse spleen and liver tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: k562 cell lysate Lane2:HL60 cell ysate Lane3:Hela cell lysate Lane4:Jurkat cell lysate Lane5: Cos1 cell lysate Lane6: Cos7 cell lysate Lane7:Mouse spleen tissue lysate Lane8:Mouse liver tissue lysate; Primary antibody: 1/500; Secondary antibody: Goat anti Rabbit IgG - H&L (HRP): 1/10000) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

ACTR3 antibody
  ACTR3 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using ACTR3 antibody. Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 mg / €335.00
  OriGene Technologies, Inc.
+2 additional images

ACTR3 (C-term) antibody
Western blot analysis of ACTR3 Antibody (C-term) (AP17090PU-N) in A2058 cell line lysates (35 µg/lane). ACTR3 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ACTR3
  Recombinant protein of human ARP3 actin-related protein 3 homolog (yeast) (ACTR3)  
ACTR3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Actr3 (GFP-tagged) - Mouse ARP3 actin-related protein 3 homolog (yeast) (Actr3), transcript variant 1, 200 uL, >10^7 TU/mL
Lenti ORF particles, Actr3 (GFP-tagged) - Mouse ARP3 actin-related protein 3 homolog (yeast) (Actr3), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Actr3 (Myc-DDK-tagged) - Mouse ARP3 actin-related protein 3 homolog (yeast) (Actr3), transcript variant 1, 200 uL, >10^7 TU/mL
Lenti ORF particles, Actr3 (Myc-DDK-tagged) - Mouse ARP3 actin-related protein 3 homolog (yeast) (Actr3), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €830.00
  OriGene Technologies, Inc.
 

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Actr3 (GFP-tagged ORF) - Rat ARP3 actin-related protein 3 homolog (yeast) (Actr3), 200 uL, >10^7 TU/mL
Lenti ORF particles, Actr3 (GFP-tagged ORF) - Rat ARP3 actin-related protein 3 homolog (yeast) (Actr3), 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Actr3 (Myc-DDK-tagged ORF) - Rat ARP3 actin-related protein 3 homolog (yeast) (Actr3), 200 uL, >10^7 TU/mL
Lenti ORF particles, Actr3 (Myc-DDK-tagged ORF) - Rat ARP3 actin-related protein 3 homolog (yeast) (Actr3), 200 uL, >10^7 TU/mL  
200 µl / €830.00
  OriGene Technologies, Inc.
 

9 Item(s)

per page

Available Filters

Product Type
  1. Mouse Lenti ORF Particle (2)
  2. Primary Antibody (4)
  3. Protein | Growth Factor (1)
  4. Rat Lenti ORF Particle (2)
Clonality
  1. Polyclonal (4)
Target
  1. ACTR3 (5)
Property
  1. C-term (1)
Reactivity
  1. Human (Hu) (4)
  2. Mouse (Ms) (3)
  3. Rat (Rt) (2)
Applications
  1. Western blot / Immunoblot (WB) (4)
  2. Enzyme Immunoassay (E) (1)
  3. Paraffin Sections (P) (1)
Presentation
  1. Purified (3)
Host
  1. Rabbit (4)
Images
  1. 1 image (5)
  2. 3+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (4)
  2. IHC (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (9)
Service
  • OriGene Technologies GmbH
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  • Germany
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  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
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  • orders: ordering-de@origene.com
 
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