ADAP1 / CENTA1 antibody
Principal name
ADAP1 / CENTA1 antibody
Alternative names for ADAP1 / CENTA1 antibody
Centaurin alpha 1, Centaurin-alpha-1, PM25, Arf-GAP with dual PH domain-containing protein 1, Putative MAPK-activating protein PM25
Ncbi ID
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of ADAP1 / CENTA1 antibody
- Kit Component:
- - KN207549G1, ADAP1 gRNA vector 1 in pCas-Guide vector
- KN207549G2, ADAP1 gRNA vector 2 in pCas-Guide vector
- KN207549D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Venkateswarlu K, Oatey PB, Tavare JM, Jackson TR, Cullen PJ.
Identification of centaurin-alpha1 as a potential in vivo phosphatidylinositol 3,4,5-trisphosphate-binding protein that is functionally homologous to the yeast ADP-ribosylation factor (ARF) GTPase-activating protein, Gcs1.
Biochem J. 1999 Jun 1;340 ( Pt 2):359-63.
PMID: 10333475
Immunofluorescence of purified MaxPab antibody to CENTA1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of ADAP1 expression in transfected 293T cell line (H00011033-T01) by ADAP1 MaxPab polyclonal antibody.Lane 1: CENTA1 transfected lysate(41.14 KDa).Lane 2: Non-transfected lysate.
ADAP1 polyclonal antibody ( Cat # PAB6491 ) staining ( 1 µg/ml ) of human brain lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
TA302895 (1µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Sample(30 ug of whole cell lysate). A: A431 . B: HeLaS3. 7.5% SDS PAGE. TA308995 diluted at 1:500
Immunohistochemical analysis of paraffin-embedded gastric tissue, using Centaurin alpha 1(TA308995) antibody at 1:100 dilution.
Predicted band size: 43 kDa . Positive control: Mouse brain tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 15 seconds)
Predicted cell location: Cytoplasm, Nucleus. Positive control: Human colon cancer tissue. Recommended dilution: 1/100-200 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue tissue using ADAP1 antibody at dilution 1/100, on the right is treated with the synthetic peptide. (Original magnification:x200)
Predicted band size: 43 kDa . Positive control: Mouse pancreas tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/162.5 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds)
Predicted cell location: Cytoplasm. Positive control: Human colon cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using ADAP1 antibody at dilution 1/40, on the right is treated with the fusion protein. (Original magnification: x200)
WB Suggested Anti-Centa1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Mouse Kidney
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302895 | ADAP1 / CENTA1 (C-term) antibody |
||||||
| Goat Polyclonal Antibody against CENTA1 | |||||||
 |
Goat | Aff - Purified | Can, Hu, Ms, Por, Rt | WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA308995 | ADAP1 / CENTA1 antibody |
||||||
| Rabbit Polyclonal antibody to Centaurin alpha 1 (ArfGAP with dual PH domains 1) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | P, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
| TA322456 | ADAP1 / CENTA1 antibody |
||||||
| Anti-ADAP1 rabbit polyclonal antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA322457 | ADAP1 / CENTA1 antibody |
||||||
| Anti-ADAP1 rabbit polyclonal antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP307549 | ADAP1 / CENTA1 |
||||
| Recombinant protein of human ArfGAP with dual PH domains 1 (ADAP1) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY416347 | ADAP1 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
