ADO antibody
Principal name
ADO antibody
Alternative names for ADO antibody
C10orf22, 2-aminoethanethiol dioxygenase, Cysteamine dioxygenase
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of ADO antibody
- Kit Component:
- - KN300917G1, Ado gRNA vector 1 in pCas-Guide vector
- KN300917G2, Ado gRNA vector 2 in pCas-Guide vector
- KN300917D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Dominy, J.E. Jr., et al. J. Biol. Chem. 282(35):25189-25198(2007)
ADO Antibody (C-term) (Cat. #AP50095PU-N) western blot analysis in mouse kidney tissue lysates (35µg/lane).This demonstrates the ADO antibody detected the ADO protein (arrow).
ADO Antibody (C-term) (Cat. #AP50095PU-N) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Gel: 8%SDS-PAGE,Lysate: 40 µg,,Primary antibody: ADO Antibody at dilution 1/300 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 5 minutes
Predicted band size: 30 kDa . Positive control: Mouse testis tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lysate: 30 ug Primary antibody: 1/1200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 minutes)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA322127 | ADO antibody |
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| Anti-ADO Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA322128 | ADO antibody |
||||||
| Anti-ADO Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP50095PU-N | ADO (C-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu, Ms | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
 +1 additional image |
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN205583 | ADO - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN205583BN | ADO - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN205583LP | ADO - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN205583RB | ADO - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP305583 | ADO |
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| Recombinant protein of human 2-aminoethanethiol (cysteamine) dioxygenase (ADO) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR203214L2V | Lenti ORF particles, Ado (GFP-tagged) - Mouse 2-aminoethanethiol (cysteamine) dioxygenase (Ado), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR203214L1V | Lenti ORF particles, Ado (Myc-DDK-tagged) - Mouse 2-aminoethanethiol (cysteamine) dioxygenase (Ado), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||