AGPAT5 antibody
Principal name
AGPAT5 antibody
Alternative names for AGPAT5 antibody
1-AGP acyltransferase 5, LPAAT-epsilon, 1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon, 1-acylglycerol-3-phosphate O-acyltransferase 5, Lysophosphatidic acid acyltransferase epsilon
Ncbi ID
9606, NP_060831, NP_060831.2, NP_081068.1, NM_026792, NM_018361
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)
Background of AGPAT5 antibody
Mitochondrial creatine kinase (MtCK) is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Sarcomeric mitochondrial creatine kinase has 80% homology with the coding exons of ubiquitous mitochondrial creatine kinase. This gene contains sequences homologous to several motifs that are shared among some nuclear genes encoding mitochondrial proteins and thus may be essential for the coordinated activation of these genes during mitochondrial biogenesis. Three transcript variants encoding the same protein have been found for this gene.This gene encodes a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. This integral membrane protein converts lysophosphatidic acid to phosphatidic acid, the second step in de novo phospholipid biosynthesis.
General readings
"Cloning and expression of LPAAT-epsilon." Leung D.W. Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
Western Blot analysis of AGPAT5 expression in transfected 293T cell line (H00055326-T01) by AGPAT5 MaxPab polyclonal antibody.Lane 1: AGPAT5 transfected lysate(40.04 KDa).Lane 2: Non-transfected lysate.
Human Muscle; WB Suggested Anti-AGPAT5 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Muscle; AGPAT5 antibody - N-terminal region (ARP47429_P050) in Human Muscle cells using Western Blot
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-AGPAT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504140)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-AGPAT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504140)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-AGPAT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504140)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-AGPAT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504140)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-AGPAT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504140)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AGPAT5 (RC210280, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AGPAT5.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-AGPAT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504142)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-AGPAT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504142)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-AGPAT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504142)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-AGPAT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504142)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AGPAT5 (RC210280, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AGPAT5.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AGPAT5 (RC210280, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AGPAT5.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-AGPAT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504144)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-AGPAT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504144)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-AGPAT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504144)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-AGPAT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504144)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-AGPAT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504144)
Anti-AGPAT5 mouse monoclonal antibody (TA504144) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AGPAT5(RC210280).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AGPAT5 (RC210280, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AGPAT5.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AGPAT5 (RC210280, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AGPAT5.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-AGPAT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504192)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-AGPAT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504192)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-AGPAT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504192)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-AGPAT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504192)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AGPAT5 (RC210280, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AGPAT5.
Flow cytometric Analysis of Jurkat cells, using anti-AGPAT5 antibody(TA504192),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Hela cells, using anti-AGPAT5 antibody(TA504192),(Red), compared to a nonspecific negative control antibody,(Blue).
Western blot analysis of extracts from LOVO cells, using AGPAT5 antibody.The lane on the right is treated with the synthesized peptide.
WB Suggested Anti-AGPAT5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human Muscle
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP310280 | AGPAT5 |
||||
| Recombinant protein of human 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) (AGPAT5) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||