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AKAP13 antibody

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Principal name

AKAP13 antibody

Alternative names for AKAP13 antibody

BRX, HT31, LBC, AKAP-Lbc, P47, A-kinase anchor protein 13, Protein kinase A-anchoring protein 13, Breast cancer nuclear receptor-binding auxiliary protein, Human thyroid-anchoring protein 31, Guanine nucleotide exchange factor Lbc, Lymphoid blast crisis oncogene, Non-oncogenic Rho GTPase-specific GTP exchange factor

SwissProt ID

Q12802 (Human)

Gene ID

11214 (AKAP13)

Ncbi ID

9606, NP_006729.4

Available reactivities

Hu (Human)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection))

Background of AKAP13 antibody

The type II cAMP-protein kinase (PKA) is a multifunctional kinase with a broad range of substrates. Specificity of PKA signaling is thought to be mediated by the compartmentalization of the kinase to specific sites within the cell. To maintain this specific localization, the R subunit (RII) of PKA interacts with specific RII-anchoring proteins. The family of RII-anchoring proteins has been designated A-kinase anchoring proteins (AKAP). AKAP 13, also known as Brx (breast cancer nuclear receptor-binding auxiliary protein), Lbc (lymphoid blast crisis oncogene), HA-3 or Ht31 (human thyroid-anchoring protein 31), functions as a cAMP-dependent scaffold anchor for PKA and also has Rho-GEF activity. It is known to regulate TLR2 signaling, NFkappaB activation, protein kinase D activation and participate in Actin stress fiber formation. Seven isoforms exist for AKAP 13 and, depending on the isoform, it localizes to the cytoplasm, nucleus or cell membrane.

H00011214-M01 - AKAP13
Immunofluorescence of monoclonal antibody to AKAP13 on HeLa cell. [antibody concentration 10 ug/ml]
H00011214-M01 - AKAP13
Detection limit for recombinant GST tagged AKAP13 is approximately 0.03ng/ml as a capture antibody.
H00011214-M10 - AKAP13
Immunoperoxidase of monoclonal antibody to AKAP13 on formalin-fixed paraffin-embedded human spleen. [antibody concentration 1.5 ug/ml]
H00011214-M10 - AKAP13
AKAP13 monoclonal antibody (M10), clone 3D6 Western Blot analysis of AKAP13 expression in K-562 ( Cat # L009V1 ).
H00011214-M10 - AKAP13
AKAP13 monoclonal antibody (M10), clone 3D6. Western Blot analysis of AKAP13 expression in PC-12(Cat # L012V1 ).
H00011214-M10 - AKAP13
Detection limit for recombinant GST tagged AKAP13 is approximately 0.03ng/ml as a capture antibody.
NB100-68213 - AKAP13
WB and IP: AKAP13/AKAP-Lbc Antibody [NB100-68213] - Detection of Human AKAP13/AKAP-Lbc on HeLa whole cell lysate using NB100-68213. AKAP13/AKAP-Lbc was also immunoprecipitated by rabbit anti-AKAP13/AKAP-Lbc antibody NB100-68214.
NB100-68214 - AKAP13
WB and IP: AKAP13/AKAP-Lbc Antibody [NB100-68214] - Detection of Human AKAP13/AKAP-Lbc on HeLa whole cell lysate using NB100-68214. AKAP13/AKAP-Lbc was also immunoprecipitated by rabbit anti-AKAP13/AKAP-Lbc antibody NB100-68213.
NBP1-00706 - AKAP13
Immunohistochemistry-Paraffin: AKAP13 Antibody [NBP1-00706] - Paraffin-embedded human lung carcinoma tissue.
NBP1-00706 - AKAP13
Western Blot: AKAP13 Antibody [NBP1-00706] - (WB) analysis of AKAP 13 (D738) antibody in extracts from Jurkat cells
AP01177PU-N - AKAP13
Immunohistochemistry (IHC) analyzes of AKAP13 antibody (Cat.-No. AP01177PU-N) in paraffin-embedded human lung carcinoma tissue.
AP01177PU-N - AKAP13
Western blot (WB) analysis of AKAP13 antibody (Cat.-No. AP01177PU-N) in extracts from Jurkat cells.
H00011214-B01P - AKAP13
Western Blot analysis of AKAP13 expression in transfected 293T cell line (H00011214-T02) by AKAP13 MaxPab polyclonal antibody.Lane 1: AKAP13 transfected lysate(116.38 KDa).Lane 2: Non-transfected lysate.
H00011214-D01P - AKAP13
Western Blot analysis of AKAP13 expression in transfected 293T cell line (H00011214-T02) by AKAP13 MaxPab polyclonal antibody.Lane 1: AKAP13 transfected lysate(120.20 KDa).Lane 2: Non-transfected lysate.
C10921-1 - AKAP13
Immunohistochemistry analysis of paraffin-embedded human heart tissue, using AKAP13 Antibody. The picture on the right is treated with the synthesized peptide.
C10921-1 - AKAP13
Western blot analysis of extracts from Jurkat/A549 cells, using AKAP13 Antibody. The lane on the right is treated with the synthesized peptide.
NBP1-89163 - AKAP13
Immunohistochemistry-Paraffin: AKAP13 Antibody [NBP1-89163] - Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
NBP1-89163 - AKAP13
Immunofluorescence: AKAP13 Antibody [NBP1-89163] Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
CPA2538-100ul - AKAP13
Immunohistochemical analysis of AKAP13 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CPA2538-100ul - AKAP13
Immunofluorescent analysis of AKAP13 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
CPA2538-100ul - AKAP13
Western blot analysis of AKAP13 expression in HeLa (A) whole cell lysates.

2 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AKAP13 antibody
  Rabbit polyclonal anti-AKAP13 antibody  
Western blot analysis of extracts from Jurkat cells and A549 cells, using AKAP13 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

AKAP13 antibody
Immunohistochemistry (IHC) analyzes of AKAP13 antibody (Cat.-No. AP01177PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH
+1 additional image

2 Item(s)

per page

Available Filters

Product Type
  1. Primary Antibody (2)
Clonality
  1. Polyclonal (2)
Target
  1. AKAP13 (2)
Reactivity
  1. Human (Hu) (2)
Applications
  1. Paraffin Sections (P) (2)
  2. Western blot / Immunoblot (WB) (2)
  3. Enzyme Immunoassay (E) (1)
Presentation
  1. Aff - Purified (1)
  2. Purified (1)
Host
  1. Rabbit (2)
Images
  1. 1 image (2)
Images: Applications
  1. Western blot / Immunoblot (WB) (2)
  2. IHC (1)
  3. Paraffin Sections (P) (1)
Manufacturer
  1. OriGene Technologies (2)
Service
  • OriGene Technologies GmbH
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