AKAP7 alpha/beta antibody
Principal name
AKAP7 alpha/beta antibody
Alternative names for AKAP7 alpha/beta antibody
AKAP7, AKAP18, A-kinase anchor protein 7 isoforms alpha and beta, Protein kinase A-anchoring protein 7 isoforms alpha/beta, A-kinase anchor protein 18
SwissProt ID
O43687 (Human)
Gene ID
9465 (AKAP7)
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of AKAP7 alpha/beta antibody
- Kit Component:
- - KN213040G1, AKAP7 gRNA vector 1 in pCas-Guide vector
- KN213040G2, AKAP7 gRNA vector 2 in pCas-Guide vector
- KN213040D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Trotter K.W., et al. (1999) J. Cell Biol. 147:1481-1492Klussmann, E., et al. (2001) Kidney Int. 60: 446-449.
Detection limit for recombinant GST tagged AKAP7 is approximately 1ng/ml as a capture antibody.
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-AKAP7 Mouse monoclonal antibody. (TA804819; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKAP7 (RC219968, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKAP7.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKAP7 (RC219968, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKAP7.
Predicted cell location: Cytoplasm. Positive control: Human brain and colon cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using AKAP7 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification:x 200)
Predicted cell location: Cytoplasm. Positive control: Human brain and colon cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using AKAP7 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification:x200)
WB Suggested Anti-AKAP7 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human brain
WB Suggested Anti-AKAP7 Antibody Titration: 1.25 ug/ml; Positive Control: HepG2 cell lysateAKAP7 is supported by BioGPS gene expression data to be expressed in HepG2
WB Suggested Anti-AKAP7 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human brain
WB Suggested Anti-AKAP7 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Small Intestine
WB Suggested Anti-AKAP7 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 721_B cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA322703 | AKAP7 alpha/beta antibody |
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| Anti-AKAP7 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA804819 | AKAP7 alpha/beta antibody |
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| AKAP7 mouse monoclonal antibody, clone OTI6F7 (formerly 6F7) | |||||||
 |
Mouse | IgG1 | OTI6F7 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA804913 | AKAP7 alpha/beta antibody |
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| AKAP7 mouse monoclonal antibody, clone OTI2A7 (formerly 2A7) | |||||||
 |
Mouse | IgG1 | OTI2A7 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP313040 | AKAP7 alpha/beta (transcript variant alpha) |
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| Recombinant protein of human A kinase (PRKA) anchor protein 7 (AKAP7), transcript variant alpha | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP323812 | AKAP7 alpha/beta (transcript variant beta) |
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| Recombinant protein of human A kinase (PRKA) anchor protein 7 (AKAP7), transcript variant beta | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||