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AKAP9 antibody

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Principal name

AKAP9 antibody

Alternative names for AKAP9 antibody

AKAP350, AKAP450, KIAA0803, Protein hyperion, Protein yotiao, AKAP120-like protein, A-kinase anchor protein 9, Protein kinase A-anchoring protein 9, A-kinase anchor protein 450 kDa, A-kinase anchor protein 350 kDa, entrosome- and Golgi-localized PKN-associated protein

SwissProt ID

F1LPB4, Q28628 (Rabit), Q70FJ1, Q99996 (Human)

Gene ID

10142 (AKAP9), 246150

Ncbi ID

NP_005742.4, AAH15533, 9606, NP_005742

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of AKAP9 antibody

AKAP9 is a novel cytoskeletal protein specifically concentrated in the neuromuscular junction and neuronal synapes that interacts with specific splice variants of NMDA Receptor Subunit NR1 in a C1 exon cassette dependent manner. It has also been shown to physically attach the type I protein phosphatase (PP1) and the adenosine 3',5'-mono-phosphate (cAMP) dependent protein kinase (PKA) holoenzyme to NMDA receptors to regulate channel activity. NMDA receptors are involved with many important functions and dysfunctions of the nervous system, including synapse formation, synaptic plasticity, and excitotoxicity. AKAP9 research may lead to a clearer understanding of these neural processes by explaining how NMDA receptors are targeted to particular synapses, how this localization is regulated during development and synaptic activity, and how NMDA receptor activity is transduced into intracellular signals responsible for particular neuronal responses.

General readings

Ciampi R, Knauf JA, Kerler R, Gandhi M, Zhu Z, Nikiforova MN, Rabes HM, Fagin JA, Nikiforov YE.
Oncogenic AKAP9-BRAF fusion is a novel mechanism of MAPK pathway activation in thyroid cancer.
J Clin Invest. 2005 Jan;115(1):94-101.
PMID: 15630448

H00010142-M01 - AKAP9
Detection limit for recombinant GST tagged AKAP9 is approximately 0.3ng/ml as a capture antibody.
ARP38677_T100 - AKAP9
Human Jurkat; WB Suggested Anti-AKAP9 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:12500. Positive Control: Jurkat cell lysate. There is BioGPS gene expression data showing that AKAP9 is expressed in Jurkat.; AKAP9 antibody - N-terminal region (ARP38677_T100) in Human Jurkat cells using Western Blot
NB100-86994 - AKAP9
Western Blot and Immunoprecipitation: AKAP9 Antibody [NB100-86994] - Detection of Human AKAP9/CG-NAP on HeLa whole cell lysate using NB100-86994. AKAP9/CGNAP was also immunoprecipitated by another rabbit anti-AKAP9/CGNAP antibody.
ARP39871_P050 - AKAP9
Rat Heart; WB Suggested Anti-Akap9 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Rat Heart; Akap9 antibody - C-terminal region (ARP39871_P050) in Rat Heart cells using Western Blot
NBP1-89166 - AKAP9
Immunohistochemistry-Paraffin: AKAP9 Antibody [NBP1-89166] - Immunohistochemical staining of human bronchus shows strong cytoplasmic positivity in respiratory epithelial cells.
NBP1-89166 - AKAP9
Immunofluorescence: AKAP9 Antibody [NBP1-89166] Immunofluorescent staining of human cell line A-431 shows positivity in golgi apparatus.
NBP1-89167 - AKAP9
Immunohistochemistry-Paraffin: AKAP9 Antibody [NBP1-89167] - Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
NBP1-89167 - AKAP9
Immunofluorescence: AKAP9 Antibody [NBP1-89167] Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm & golgi apparatus.
GTX79473 - AKAP9
Immunohistochemistry was performed on normal biopsies of deparaffinized human pancreas tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed at a dilution of 1:20 with or without AKAP450 antibody [17G10] overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

2 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AKAP9 antibody
  Anti-AKAP9 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human lung cancer tissue. Recommended dilution: 1/10-50 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using AKAP9 antibody at dilution 1/10, on the right is treated with the fusion protein. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.

AKAP9 (C-term) antibody
AP16328PU-N Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml). Goat Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €380.00
  OriGene Technologies GmbH
+1 additional image

2 Item(s)

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Available Filters

Product Type
  1. Primary Antibody (2)
Clonality
  1. Polyclonal (2)
Target
  1. AKAP9 (2)
Property
  1. C-term (1)
Reactivity
  1. Human (Hu) (2)
  2. Mouse (Ms) (2)
  3. Rat (Rt) (1)
Applications
  1. Enzyme Immunoassay (E) (2)
  2. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  3. Paraffin Sections (P) (1)
  4. Western blot / Immunoblot (WB) (1)
Presentation
  1. Aff - Purified (1)
  2. Purified (1)
Host
  1. Goat (1)
  2. Rabbit (1)
Images
  1. 1 image (2)
Images: Applications
  1. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  2. IHC (1)
Manufacturer
  1. OriGene Technologies (2)
Service
  • OriGene Technologies GmbH
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  • Germany
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