AKR1C4 antibody
Principal name
AKR1C4 antibody
Alternative names for AKR1C4 antibody
Chlordecone reductase, CDR, CHDR, 3-alpha-HSD1, Dihydrodiol dehydrogenase 4, DD4, DD-4, HAKRA, Aldo-keto reductase family 1 member C4, 3-alpha-hydroxysteroid dehydrogenase type I
SwissProt ID
P17516 (Human)
Gene ID
1109 (AKR1C4)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP)
Background of AKR1C4 antibody
- Kit Component:
- - KN204731G1, AKR1C4 gRNA vector 1 in pCas-Guide vector
- KN204731G2, AKR1C4 gRNA vector 2 in pCas-Guide vector
- KN204731D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Stayrook KR. et al. (2008) Mol Pharmacol. 73:607-612.
Immunoperoxidase of monoclonal antibody to AKR1C4 on formalin-fixed paraffin-embedded human liver tissue. [antibody concentration 3ug/ml]
Immunofluorescence of monoclonal antibody to AKR1C4 on HepG2 cell. [antibody concentration 15 ug/ml]
Western Blot analysis of AKR1C4 expression in transfected 293T cell line by AKR1C4 monoclonal antibody (M01), clone 2C11.Lane 1: AKR1C4 transfected lysate(37.2 KDa).Lane 2: Non-transfected lysate.
AKR1C4 monoclonal antibody (M01), clone 2C11 Western Blot analysis of AKR1C4 expression in MCF-7 ( Cat # L046V1 ).
AKR1C4 polyclonal antibody ( Cat # PAB7023 ) ( 0.1 µg/ml ) staining of human liver lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
AKR1C4 MaxPab rabbit polyclonal antibody. Western Blot analysis of AKR1C4 expression in human liver.
Western Blot analysis of AKR1C4 expression in transfected 293T cell line (H00001109-T01) by AKR1C4 MaxPab polyclonal antibody.Lane 1: AKR1C4 transfected lysate(37.20 KDa).Lane 2: Non-transfected lysate.
Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Antibody (0.1 µg/ml) staining of human liver lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
AKR1C4 MaxPab polyclonal antibody. Western Blot analysis of AKR1C4 expression in human liver.
Western Blot analysis of AKR1C4 expression in transfected 293T cell line (H00001109-T01) by AKR1C4 MaxPab polyclonal antibody.Lane 1: AKR1C4 transfected lysate(35.53 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of AKR1C4 expression in transfected 293T cell line (H00001109-T01) by AKR1C4 MaxPab polyclonal antibody.Lane 1: AKR1C4 transfected lysate(37.20 KDa).Lane 2: Non-transfected lysate.
AKR1C4 MaxPab rabbit polyclonal antibody. Western Blot analysis of AKR1C4 expression in human liver.
Immunoprecipitation of AKR1C4 transfected lysate using anti-AKR1C4 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with AKR1C4 MaxPab mouse polyclonal antibody (B01) (H00001109-B01).
Human Tonsil, Squamous Epithelium: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence of monoclonal antibody to AKR1C4 on HepG2 cell. [antibody concentration 15 µg/ml]
AKR1C4 monoclonal antibody, clone 2C11 Western Blot analysis of AKR1C4 expression in MCF-7.
Western Blot analysis of AKR1C4 expression in transfected 293T cell line by AKR1C4 monoclonal antibody, clone 2C11.
Recombinant human AKR1C4, 1-323aa, His-tagged
AKR1C4 Antibody (N-term) (Cat. #AP50134PU-N) western blot analysis in NCI-H292 cell line lysates (35 µg/lane).This demonstrates the AKR1C4 antibody detected the AKR1C4 protein (arrow).
TA303107 (0.1µg/ml) staining of human liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Sample (30 ug of whole cell lysate). A: Hela. 10% SDS PAGE. HSD3a antibody. TA307983 diluted at 1:1000.
Immunohistochemical analysis of paraffin-embedded gastric ca, using AKR1C4(TA307983) antibody at 1:100 dilution.
HSD3a antibody [N1C2] detects HSD3a protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HSD3a protein stained by HSD3a antibody [N1C2] (TA307983) diluted at 1:500. B
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM31157PU-N | AKR1C4 antibody |
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Mouse | IgG1 | 2C11 | Purified | Hu | E, ICC/IF, P, WB |
50 µg /
€475.00
|
| OriGene Technologies GmbH | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA303107 | AKR1C4 (N-term) antibody |
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| Goat Polyclonal Antibody against AKR1C4 | |||||||
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Goat | Aff - Purified | Hu | P, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA307983 | AKR1C4 antibody |
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| Rabbit polyclonal antibody to HSD3a (aldo-keto reductase family 1, member C4 (chlordecone reductase; 3-alpha hydroxysteroid dehydrogenase, type I; dihydrodiol dehydrogenase 4)) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | P, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| AP08974PU-N | AKR1C4 (2-14) antibody |
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 |
Goat | Aff - Purified | Hu | E, P, WB |
50 µg /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP50134PU-N | AKR1C4 (N-term) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP304731 | AKR1C4 |
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| Recombinant protein of human aldo-keto reductase family 1, member C4 (chlordecone reductase; 3-alpha hydroxysteroid dehydrogenase, type I; dihydrodiol dehydrogenase 4) (AKR1C4) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP720536 | AKR1C4 |
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| Recombinant protein of human aldo-keto reductase family 1, member C4 (chlordecone reductase; 3-alpha hydroxysteroid dehydrogenase, type I; dihydrodiol dehydrogenase 4) (AKR1C4) | |||||
 |
Human | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
10 µg /
€299.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY419725 | AKR1C4 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
