ALDH4 antibody
Principal name
ALDH4 antibody
Alternative names for ALDH4 antibody
ALDH4A1, P5CDH, P5C dehydrogenase, Delta-1-pyrroline-5-carboxylate dehydrogenase mitochondrial, Aldehyde dehydrogenase family 4 member A1
Ncbi ID
9606, CAI23418, NP_003739, NM_001134698, NP_001154976.1, NP_733844, NM_170726, NM_003748
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of ALDH4 antibody
ALDH4A1 belongs to the aldehyde dehydrogenase family of proteins. This enzyme is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Deficiency of this enzyme is associated with type II hyperprolinemia, an autosomal recessive disorder characterized by accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline.This protein belongs to the aldehyde dehydrogenase family of proteins. This enzyme is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Deficiency of this enzyme is associated with type II hyperprolinemia, an autosomal recessive disorder characterized by accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. Two transcript variants encoding the same protein have been identified for this gene.
General readings
Yoon,K.A., (2004) J. Hum. Genet. 49 (3), 134-140
Immunoperoxidase of monoclonal antibody to ALDH4A1 on formalin-fixed paraffin-embedded human liver tissue.[antibody concentration 5ug/ml]
Western Blot analysis of ALDH4A1 expression in transfected 293T cell line by ALDH4A1 monoclonal antibody (M01), clone 1A12-A5.Lane 1: ALDH4A1 transfected lysate(61.7 KDa).Lane 2: Non-transfected lysate.
ALDH4A1 monoclonal antibody (M01), clone 1A12-A5 Western Blot analysis of ALDH4A1 expression in Hela ( Cat # L013V1 ).
Detection limit for recombinant GST tagged ALDH4A1 is approximately 0.1ng/ml as a capture antibody.
ALDH4A1 MaxPab polyclonal antibody. Western Blot analysis of ALDH4A1 expression in human liver.
Western Blot analysis of ALDH4A1 expression in transfected 293T cell line (H00008659-T01) by ALDH4A1 MaxPab polyclonal antibody.Lane 1: ALDH4A1 transfected lysate(61.93 KDa).Lane 2: Non-transfected lysate.
ALDH4A1 MaxPab polyclonal antibody. Western Blot analysis of ALDH4A1 expression in HepG2.
Western Blot analysis of ALDH4A1 expression in transfected 293T cell line (H00008659-T02) by ALDH4A1 MaxPab polyclonal antibody.Lane 1: ALDH4A1 transfected lysate(61.70 KDa).Lane 2: Non-transfected lysate.
ALDH4A1 MaxPab rabbit polyclonal antibody. Western Blot analysis of ALDH4A1 expression in HeLa.
Human Intestine; ALDH4A1 antibody - N-terminal region (ARP45696_T100) in Human Intestine cells using Immunohistochemistry
Human HepG2; WB Suggested Anti-ALDH4A1 Antibody Titration: 2.5ug/ml. Positive Control: HepG2 cell lysate. ALDH4A1 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells.; ALDH4A1 antibody - N-terminal region (ARP45696_T100) in Human HepG2 cells using Western Blot
Human Muscle; ALDH4A1 antibody - C-terminal region (ARP45697_T100) in Human Muscle cells using Immunohistochemistry
Human Fetal liver; WB Suggested Anti-ALDH4A1 Antibody Titration: 1.25 ug/ml. Positive Control: Fetal liver cell lysate; ALDH4A1 antibody - C-terminal region (ARP45697_T100) in Human Fetal liver cells using Western Blot
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ALDH4A1 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-ALDH4A1 Antibody (C-term) in HepG2 cell line lysates (35ug/lane). ALDH4A1(arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ALDH4A1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-ALDH4A1 Antibody (Center) in HepG2 cell line lysates (35ug/lane). ALDH4A1(arrow) was detected using the purified Pab.
ALDH4A1 MaxPab polyclonal antibody. Western Blot analysis of ALDH4A1 expression in human liver.
Western Blot analysis of ALDH4A1 expression in transfected 293T cell line (H00008659-T01) by ALDH4A1 MaxPab polyclonal antibody.Lane 1: ALDH4A1 transfected lysate(61.93 KDa).Lane 2: Non-transfected lysate.
ALDH4A1 MaxPab polyclonal antibody. Western Blot analysis of ALDH4A1 expression in HepG2.
Rat Kidney; WB Suggested Anti-Aldh4a1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Rat Kidney; Aldh4a1 antibody - N-terminal region (ARP45695_P050) in Rat Kidney cells using Western Blot
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-ALDH4A1 rabbit polyclonal antibody. (TA590378)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALDH4A1 (RC204884, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALDH4A1.
ELISA: ALDH4A1 Antibody
Predicted band size: 62kDa. Positive control: Human fetal liver and liver cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: Human fetal liver tissue lysate Lane2: Human liver cancer tissue lysate Lysates: 40 ug per lane Primary antibody: 1/450 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 30 seconds)
Predicted cell location: Cytoplasm. Positive control: Human breast cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using ALDH4A1 antibody at dilution 1/45, on the right is treated with the fusion protein. (Original magnification:x200)
Predicted band size: 62kDa. Positive control: Human fetal liver tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 30 seconds)
Predicted cell location: Cytoplasm. Positive control: Human breast cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using ALDH4A1 antibody at dilution 1/35, on the right is treated with the fusion protein. (Original magnification:x200)
Western blot analysis of extracts of various cell lines, using ALDH4A1 antibody.
Immunohistochemistry of paraffin-embedded human breast cancer using ALDH4A1 antibody at dilution of 1:200 (200x lens).
Immunohistochemistry of paraffin-embedded human thyroid cancer using ALDH4A1 antibody at dilution of 1:200 (200x lens).
Immunohistochemistry of paraffin-embedded mouse lung using ALDH4A1 antibody at dilution of 1:200 (200x lens)
Immunofluorescence analysis of A549 cell using ALDH4A1 antibody.
WB Suggested Anti-Aldh4a1 Antibody; Titration: 1.0 ug/ml; Positive Control: Rat Kidney
WB Suggested Anti-ALDH4A1 Antibody Titration: 1.25 ug/ml; Positive Control: Fetal liver cell lysate
Human Muscle
WB Suggested Anti-ALDH4A1 Antibody Titration: 2.5 ug/ml; Positive Control: HepG2 cell lysate. ALDH4A1 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells
Human Intestine
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP304884 | ALDH4 (transcript variant P5CDhS) |
||||
| Recombinant protein of human aldehyde dehydrogenase 4 family, member A1 (ALDH4A1), nuclear gene encoding mitochondrial protein, transcript variant P5CDhS | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP320893 | ALDH4 (transcript variant P5CDhL) |
||||
| Recombinant protein of human aldehyde dehydrogenase 4 family, member A1 (ALDH4A1), nuclear gene encoding mitochondrial protein, transcript variant P5CDhL | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
