ALKBH1 antibody
Principal name
ALKBH1 antibody
Alternative names for ALKBH1 antibody
ALKBH, ABH, Alkylated DNA repair protein alkB homolog 1
SwissProt ID
Q13686 (Human)
Gene ID
8846 (ALKBH1)
Ncbi ID
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Enzyme Immunoassay (E)
Background of ALKBH1 antibody
- Kit Component:
- - KN206529G1, ALKBH1 gRNA vector 1 in pCas-Guide vector
- KN206529G2, ALKBH1 gRNA vector 2 in pCas-Guide vector
- KN206529D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
"Human AlkB homolog 1 is a mitochondrial protein that demethylates 3-methylcytosine in DNA and RNA." Westbye M.P., Feyzi E., Aas P.A., Vagbo C.B., Talstad V.A., Kavli B., Hagen L., Sundheim O., Akbari M., Liabakk N.B., Slupphaug G., Otterlei M., Krokan H.E. J. Biol. Chem. 283:25046-25056(2008)
Western Blot analysis of ALKBH1 expression in transfected 293T cell line (H00008846-T02) by ALKBH1 MaxPab polyclonal antibody.Lane 1: ALKBH transfected lysate(42.79 KDa).Lane 2: Non-transfected lysate.
ALKBH1 MaxPab polyclonal antibody. Western Blot analysis of ALKBH1 expression in Jurkat.
Human Fetal Kidney; WB Suggested Anti-ALKBH1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Fetal Kidney; ALKBH1 antibody - N-terminal region (ARP61830_P050) in Human Fetal Kidney cells using Western Blot
Human Fetal Kidney; WB Suggested Anti-ALKBH1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Fetal Kidney; ALKBH1 antibody - N-terminal region (AP46320PU-N) in Human Fetal Kidney cells using Western Blot
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ALKBH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805636)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ALKBH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805636)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ALKBH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805682)
Sample (30 ug of whole cell lysate). A: NT2D1. 10% SDS PAGE. TA308976 diluted at 1:1000.
Predicted band size: 44 kDa . Positive control: Mouse pancreas tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Predicted band size: 44 kDa . Positive control: Mouse pancreas tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308976 | ALKBH1 antibody |
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| Rabbit polyclonal antibody to ABH1 (alkB, alkylation repair homolog 1 (E. coli)) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA321739 | ALKBH1 antibody |
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| Anti-ALKBH1 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA321740 | ALKBH1 antibody |
||||||
| Anti-ALKBH1 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA805636 | ALKBH1 antibody |
||||||
| ALKBH1 mouse monoclonal antibody, clone OTI4C4 (formerly 4C4) | |||||||
 |
Mouse | IgG1 | OTI4C4 | Purified | Hu | P |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|
||||||
| TA805682 | ALKBH1 antibody |
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| ALKBH1 mouse monoclonal antibody, clone OTI1B8 (formerly 1B8) | |||||||
 |
Mouse | IgG1 | OTI1B8 | Purified | Hu | P |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP306529 | ALKBH1 |
||||
| Recombinant protein of human alkB, alkylation repair homolog 1 (E. coli) (ALKBH1) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||