ALOX15B antibody
Principal name
ALOX15B antibody
Alternative names for ALOX15B antibody
15-LOX-2, Arachidonate 15-lipoxygenase type II
Ncbi ID
9606, NP_001132, 10090, NP_001034220, NP_001034219, NP_001034219.1, NM_001141, NP_033791.1, NM_009661, NM_153301
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Immunoprecipitation (IP), Paraffin Sections (P)
Background of ALOX15B antibody
- Kit Component:
- - KN211380G1, ALOX15B gRNA vector 1 in pCas-Guide vector
- KN211380G2, ALOX15B gRNA vector 2 in pCas-Guide vector
- KN211380D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Subbarayan,V., (2005) Neoplasia 7 (3), 280-293
Detection limit for recombinant GST tagged ALOX15B is approximately 10ng/ml as a capture antibody.
Human Jurkat; WB Suggested Anti-ALOX15B Antibody Titration: 2.5ug/ml. Positive Control: Jurkat cell lysate; ALOX15B antibody - C-terminal region (ARP41895_T100) in Human Jurkat cells using Western Blot
Human HepG2; WB Suggested Anti-ALOX15B Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; ALOX15B antibody - N-terminal region (ARP41894_P050) in Human HepG2 cells using Western Blot
Human Brain; WB Suggested Anti-ALOX15B Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human brain; ALOX15B antibody - middle region (ARP41918_P050) in Human Brain cells using Western Blot
Human HepG2; WB Suggested Anti-ALOX15B Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; ALOX15B antibody - middle region (ARP41919_P050) in Human HepG2 cells using Western Blot
Formalin-fixed and paraffin-embedded human skin tissue reacted with PSMD13 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of ALOX15B Antibody (C-term) (Cat. #AP50153PU-N) in CEM cell line lysates (35µg/lane). ALOX15B (arrow) was detected using the purified Pab.
Predicted cell location: Cytoplasm. Positive control: Human liver cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using ALOX15B antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification:x200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA323365 | ALOX15B antibody |
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| Anti-ALOX15B Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP50153PU-N | ALOX15B (C-term) antibody |
||||||
 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
 +1 additional image |
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Lysates
| Catalog No. | ||
|---|---|---|
| LY400457 | ALOX15B overexpression lysate |
|
 |
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR209964L2V | Lenti ORF particles, Alox8 (GFP-tagged) - Mouse arachidonate 8-lipoxygenase (Alox8), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,430.00
|
|
| OriGene Technologies, Inc. | ||
| MR209964L1V | Lenti ORF particles, Alox8 (Myc-DDK-tagged) - Mouse arachidonate 8-lipoxygenase (Alox8), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,280.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR205342L2V | Lenti ORF particles, Alox15b (GFP-tagged ORF) - Rat arachidonate 15-lipoxygenase, type B (Alox15b), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,430.00
|
|
| OriGene Technologies, Inc. | ||
| RR205342L1V | Lenti ORF particles, Alox15b (Myc-DDK-tagged ORF) - Rat arachidonate 15-lipoxygenase, type B (Alox15b), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,290.00
|
|
| OriGene Technologies, Inc. | ||