Alpha-Internexin antibody
Principal name
Alpha-Internexin antibody
Alternative names for Alpha-Internexin antibody
Alpha-Inx, INA, NEF5, NF66, 66 kDa neurofilament protein, Neurofilament-66
Ncbi ID
NP_116116.1, 9606, 10116, NP_116116, NM_032727, NP_666212.3, NM_146100, NM_019128
Available reactivities
Available hosts
Available applications
Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP)
Background of Alpha-Internexin antibody
In mammalian CNS, five key neurol intermediate filament (NIF) proteins have been discovered which includes alpha-internexin (66 kD, I or Inexa), peripherin (57 kD), and three neurofilament (NF) proteins, which are neurofilament light (NF-L, 68 kD), medium (NF-M, 145 kD), and heavy (NF-H, 200 kD). Among NIFs, alpha-internexin is categorized as class IV NIF which is highly expressed in most neurons during development especially when they begin to differentiate and before the expression of NF triplet proteins, and it is structurally/functiolly associated with NF triplet proteins inside mature CNS neurons. Peripherin is mainly expressed in PNS and in some CNS neurons also, and alpha-internexin - peripherin self-assemble or co-assemble with neurofilament protein subunits to form filamentous structure before their translocation into the axons to become an essential constituent of shape-maintaining IF network in mature neurons. It has been recognized as one of the many proteins phosphorylated by ATM/ATR in response to D damage and has been associated with developmental, degenerative /overexpression causes neurol cell death, and inflammatory processes of CNS. Abnormal NIF accumulation is a neuropathological sigture of several neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, dementia with Lewy bodies, and amyotrophic lateral sclerosis. Alpha-internexin has been detected as a major component of pathological inclusions in frontotemporal dementia, also called 'neurol intermediate filament inclusion disease (NIFID).
Formalin-fixed and paraffin-embedded human brain tissue reacted with INA antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of INA (arrow) using rabbit polyclonal INA Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the INA gene (Lane 2)
Mixed cultures of rat CNS cells stained with Ina monoclonal antibody, clone 1D2 ( Cat # MAB2316 ) ( red ) and chicken antibody to Microtubule associated protein 2 ( MAP2- green ). The a-internexin is localized primarily in neuronal axons in these cultures, while the perikarya and dendrites of neurons stain strongly for MAP2.
Western blot of whole rat spinal cord homogenate stained with Ina polyclonal antibody ( Cat # PAB14168 ), at dilution of 1 : 20,000.A prominent band running at ~ 66kDa is apparent, as well as smaller lower bands which are apparently degradation products.A minor band at ~ 150kDa is also seen, apparently resulting from dimerization of alpha-internexin.
Mixed neuron-glial cultures stained with Ina polyclonal antibody ( Cat # PAB14168 ) ( red ) and Chicken Peripherin antibody ( green ).The Ina antibody stains numerous axonal and dendritic profiles in these cultures, while peripherin antibody binds to only a subset of neurons.
Western blot of rat cortex lysate showing specific immunolabeling of the ~ 66k alpha internexin protein
Western blot of rat cortex lysate showing specific immunolabeling of the ~ 66k alpha internexin protein.
Western blot analysis of extracts from K562 cells, using INA antibody.The lane on the right is treated with the synthesized peptide.
Predicted band size: 55 kDa. Positive control: Mouse brain tissue lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Predicted cell location: Cytoplasm. Positive control: Human gastric cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human gastric cancer tissue using INA antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x200)
Predicted band size: 55 kDa. Positive control: Mouse brain tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/550 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 3 seconds)
Predicted cell location: Cytoplasm. Positive control: Human brain tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using INA antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x200)
Predicted cell location: Cytoplasm. Positive control: Human lung and renal cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using INA antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200)
Predicted cell location: Cytoplasm. Positive control: Human lung and renal cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human renal cancer tissue using INA antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x200)
Predicted band size: 55 kDa . Positive control: Mouse brain tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lysate: 50 ug per lane Primary antibody: 1/800 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 seconds)
Predicted cell location: Cytoplasm. Positive control: Human colon cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using INA antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)
INA Antibody (Cat. #TA324344) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the INA antibody detected the INA protein (arrow).
INA Antibody (Cat. #TA324344)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of INA Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of INA Antibody (Cat#TA324344) with brain tissue followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
Western Blot: alpha Internexin Antibody [TA336609] - Western blot of whole rat spinal cord homogenate stained with RPCA-a-Int, at dilution of 1:20,000. A prominent band running at ~66kDa is apparent, as well as smaller lower bands which are apparently deg
Immunocytochemistry/Immunofluorescence: alpha Internexin Antibody [TA336609] - IF Confocal analysis of SHSY5Y cells using alpha Internexin antibody (TA336609, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). A
Immunocytochemistry/Immunofluorescence: alpha Internexin Antibody [TA336609] - Mixed neuron-glial cultures stained with rabbit antibody to alpha-internexin (red) and chicken antibody to peripherin CPCA-Peri (green). The alpha internexin antibody stains nu
Immunocytochemistry/Immunofluorescence: alpha Internexin Antibody [TA336609] - Polyclonal alpha internexin, NB 300-139 (red) staining neuronal progenitor cells. The green stain shows the fibroblast marker, Plectin (not one of our antibodies).
Western Blot: alpha Internexin Antibody (2E3) - Neuronal Markers [TA336610] - blot of homogenate of rat facial nucleus incubated with NBP1-05206, which reveals a single clean band running at approx. 66kDa.
Immunohistochemistry: alpha Internexin Antibody (2E3) - Neuronal Markers [TA336610] - Rat facial nucleus before (left) and after (right) facial nerve lesion. Note the profound upregulation of a-internexin in the facial nucleus motor neurons.
Immunocytochemistry/Immunofluorescence: alpha Internexin Antibody (2E3) [TA336610] - ppocampal neurons in tissue culture with neurofilament alpha internexin (red), using NB 300-140 and GFAP (green), using NB 300-141.
Immunocytochemistry/Immunofluorescence: alpha Internexin Antibody (1D2) [TA336618] - Mixed cultures of rat CNS cells stained with TA336618 (red) and chicken antibody to Microtubule associated protein 2 (MAP2- green). The a-internexin is localized primaril
Western Blot: alpha Internexin Antibody (2E4) [TA337202] - Analysis of alpha-internexin/NF66 (clone 2E3) in crude homogenates of rat facial nucleus. Image kindly provided by G. Shaw of Florida University.
Immunohistochemistry: alpha Internexin Antibody (2E4) [TA337202] - Staining of control and leisoned rat facial nucleus with alpha-internexin/NF66 antibody, clone 2E3
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA336609 | Alpha-Internexin antibody |
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| Rabbit Polyclonal alpha-Internexin Antibody | |||||||
![Western Blot: alpha Internexin Antibody [TA336609] - Western blot of whole rat spinal cord homogenate stained with RPCA-a-Int, at dilution of 1:20,000. A prominent band running at ~66kDa is apparent, as well as smaller lower bands which are apparently deg](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NB300-139-1-W.jpg) |
Rabbit | Purified | Hu, Mam, Ms, Rt | C, ICC/IF, IP, WB |
0.1 ml /
€395.00
|
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| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA309074 | Alpha-Internexin antibody |
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| mouse Anti-Alpha Internexin/ NF-66 Antibody | |||||||
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Mouse | IgG1 | ID2 | Ig Fraction | Mam | C, ICC/IF, P, WB |
0.1 ml /
€355.00
|
| OriGene Technologies, Inc. | |||||||
| TA309075 | Alpha-Internexin antibody |
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| chicken Anti-Alpha Internexin/ NF-66 Antibody | |||||||
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Chicken | Ig | Ig Fraction | Hu, Ms, Rt | C, ICC/IF, WB |
0.1 ml /
€355.00
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| OriGene Technologies, Inc. | |||||||
| TA312641 | Alpha-Internexin antibody |
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| Rabbit polyclonal anti-INA antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| TA322129 | Alpha-Internexin antibody |
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| Anti-INA Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA322130 | Alpha-Internexin antibody |
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| Anti-INA Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA322131 | Alpha-Internexin antibody |
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| Anti-INA Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA322132 | Alpha-Internexin antibody |
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| Anti-INA Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA324344 | Alpha-Internexin antibody |
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| Mouse monoclonal INA Antibody | |||||||
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Mouse | IgG1 | 257CT7.1.2 | Purified | Bov, Ms, Rt | ICC/IF, P, WB |
0.4 ml /
€415.00
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA336610 | Alpha-Internexin antibody |
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| Mouse Monoclonal alpha-Internexin Antibody (2E3) | |||||||
![Western Blot: alpha Internexin Antibody (2E3) - Neuronal Markers [TA336610] - blot of homogenate of rat facial nucleus incubated with NBP1-05206, which reveals a single clean band running at approx. 66kDa.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NB300-140-1-W.jpg) |
Mouse | IgG1 | 2E3 | Purified | Bov, Hu, Ms, Por, Rt | C, ICC/IF, P, WB |
0.5 ml /
€450.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA336618 | Alpha-Internexin antibody |
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| Mouse Monoclonal alpha-Internexin Antibody (1D2) | |||||||
![Immunocytochemistry/Immunofluorescence: alpha Internexin Antibody (1D2) [TA336618] - Mixed cultures of rat CNS cells stained with TA336618 (red) and chicken antibody to Microtubule associated protein 2 (MAP2- green). The a-internexin is localized primaril](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NB300-216-1-F.jpg) |
Mouse | IgG1 | 1D2 | Purified | Hu, Mam, Ms, Rt | C, P, WB |
0.5 ml /
€395.00
|
| OriGene Technologies, Inc. | |||||||
| AP13281PU-N | Alpha-Internexin antibody |
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Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| RA22102-100 | Alpha-Internexin antibody |
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Rabbit | IgG | Liquid | Hu, Ms, Rt | C, E, WB |
0.1 ml /
€360.00
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| Neuromics Antibodies | |||||||
| +1 additional image |
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Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR208054L2V | Lenti ORF particles, Ina (GFP-tagged) - Mouse internexin neuronal intermediate filament protein, alpha (Ina), 200 uL, >10^7 TU/mL |
|
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200 µl /
€1,190.00
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| OriGene Technologies, Inc. | ||
| MR208054L1V | Lenti ORF particles, Ina (Myc-DDK-tagged) - Mouse internexin neuronal intermediate filament protein, alpha (Ina), 200 uL, >10^7 TU/mL |
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200 µl /
€1,060.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR215311L2V | Lenti ORF particles, Ina (GFP-tagged ORF) - Rat internexin neuronal intermediate filament protein, alpha (Ina), 200 uL, >10^7 TU/mL |
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200 µl /
€1,190.00
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| OriGene Technologies, Inc. | ||
| RR215311L1V | Lenti ORF particles, Ina (Myc-DDK-tagged ORF) - Rat internexin neuronal intermediate filament protein, alpha (Ina), 200 uL, >10^7 TU/mL |
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200 µl /
€1,070.00
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| OriGene Technologies, Inc. | ||
