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ALPPL2 antibody

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Principal name

ALPPL2 antibody

Alternative names for ALPPL2 antibody

ALPPL, Alkaline phosphatase Nagao isozyme, PLAP-like, ALP-1, Alkaline phosphatase placental-like, Germ cell alkaline phosphatase

SwissProt ID

P10696 (Human)

Gene ID

251 (ALPPL2)

Ncbi ID

9606, NP_112603, NP_112603.2, NM_031313

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of ALPPL2 antibody

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The exact physiological function of the alkaline phosphatases is not known. ALPPL2 is a membrane bound glycosylated enzyme, localized to testis, thymus and certain germ cell tumors, that is closely related to both the placental and intestinal forms of alkaline phosphatase.There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The exact physiological function of the alkaline phosphatases is not known. The product of this gene is a membrane bound glycosylated enzyme, localized to testis, thymus and certain germ cell tumors, that is closely related to both the placental and intestinal forms of alkaline phosphatase.

General readings

"Seminoma-derived Nagao isozyme is encoded by a germ-cell alkaline phosphatase gene." Millan J.L., Manes T. Proc. Natl. Acad. Sci. U.S.A. 85:3024-3028(1988)
"Expression of the germ cell alkaline phosphatase gene in human choriocarcinoma cells." Watanabe S., Watanabe T., Li W.L., Soong B.-W., Chou J.Y. J. Biol. Chem. 264:12611-12619(1989)

H00000251-M07 - ALPPL2
Immunoperoxidase of monoclonal antibody to ALPPL2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
H00000251-M07 - ALPPL2
Western blot analysis of ALPPL2 over-expressed 293 cell line, cotransfected with ALPPL2 Validated Chimera RNAi ( Cat # H00000251-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ALPPL2 monoclonal antibody (M07), clone 2B3 (Cat # H00000251-M07 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
H00000251-M07 - ALPPL2
Western Blot analysis of ALPPL2 expression in transfected 293T cell line by ALPPL2 monoclonal antibody (M07), clone 2B3.Lane 1: ALPPL2 transfected lysate(57.5 KDa).Lane 2: Non-transfected lysate.
H00000251-M07 - ALPPL2
ALPPL2 monoclonal antibody (M07), clone 2B3 Western Blot analysis of ALPPL2 expression in A-431 ( Cat # L015V1 ).
H00000251-B01P - ALPPL2
Western Blot analysis of ALPPL2 expression in transfected 293T cell line (H00000251-T01) by ALPPL2 MaxPab polyclonal antibody.Lane 1: ALPPL2 transfected lysate(58.52 KDa).Lane 2: Non-transfected lysate.
H00000251-B01P - ALPPL2
ALPPL2 MaxPab polyclonal antibody. Western Blot analysis of ALPPL2 expression in A-431.
H00000251-D01P - ALPPL2
Western Blot analysis of ALPPL2 expression in transfected 293T cell line (H00000251-T01) by ALPPL2 MaxPab polyclonal antibody.Lane 1: ALPPL2 transfected lysate(57.50 KDa).Lane 2: Non-transfected lysate.
H00000251-D01P - ALPPL2
ALPPL2 MaxPab rabbit polyclonal antibody. Western Blot analysis of ALPPL2 expression in A-431.
ARP42668_T100 - ALPPL2
Human HepG2; WB Suggested Anti-ALPPL2 Antibody Titration: 2.5ug/ml. Positive Control: HepG2 cell lysate; ALPPL2 antibody - N-terminal region (ARP42668_T100) in Human HepG2 cells using Western Blot
H00000251-D01 - ALPPL2
ALPPL2 MaxPab rabbit polyclonal antibody. Western Blot analysis of ALPPL2 expression in A-431.
H00000251-D01 - ALPPL2
Immunoprecipitation of ALPPL2 transfected lysate using anti-ALPPL2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ALPPL2 MaxPab mouse polyclonal antibody (B01) (H00000251-B01).
ARP42669_P050 - ALPPL2
Human OVCAR-3; WB Suggested Anti-ALPPL2 Antibody Titration: 0.2-1 ug/ml. Positive Control: OVCAR-3 cell lysate; ALPPL2 antibody - middle region (ARP42669_P050) in Human OVCAR-3 cells using Western Blot
AP44641PU-N - ALPPL2
Human OVCAR-3; WB Suggested Anti-ALPPL2 Antibody Titration: 0.2-1 ug/ml. Positive Control: OVCAR-3 cell lysate; ALPPL2 antibody - middle region (AP44641PU-N) in Human OVCAR-3 cells using Western Blot
TA590442 - ALPPL2
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
TA590442 - ALPPL2
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ALPPL2 Rabbit Polyclonal antibody. (TA590442)
TA590442 - ALPPL2
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ALPPL2 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
TA590442 - ALPPL2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALPPL2 (RC204330, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALPPL2.
TA590442 - ALPPL2
ELISA: ALPPL2 Antibody
TP304330 - ALPPL2
TA308201 - ALPPL2
Sample (30 ug of whole cell lysate). A: A549. 7.5% SDS PAGE. ALPPL2 antibody. TA308201 diluted at 1:1000.
TA308201 - ALPPL2
Immunohistochemical analysis of paraffin-embedded Breast, using ALPPL2(TA308201) antibody at 1:500 dilution.
TA308201 - ALPPL2
Immunofluorescence analysis of methanol-fixed A549, using ALPPL2(TA308201) antibody at 1:500 dilution.
TA322158 - ALPPL2
Predicted cell location: Cytoplasm. Positive control: Human esophagus and lung cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human esophagus cancer tissue using ALPPL2 antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification:x 200)
TA322158 - ALPPL2
Predicted cell location: Cytoplasm. Positive control: Human esophagus and lung cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using ALPPL2 antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification:x200)

4 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ALPPL2 antibody
  Rabbit Polyclonal antibody to ALPPL2 (alkaline phosphatase, placental-like 2)  
Sample (30 ug of whole cell lysate). A: A549. 7.5% SDS PAGE. ALPPL2 antibody. TA308201 diluted at 1:1000. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €415.00
  OriGene Technologies, Inc.
+2 additional images

ALPPL2 antibody
  Anti-ALPPL2 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human esophagus and lung cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human esophagus cancer tissue using ALPPL2 antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ALPPL2
  Recombinant protein of human alkaline phosphatase, placental-like 2 (ALPPL2)  
ALPPL2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

ALPPL2 overexpression lysate
ALPPL2 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

4 Item(s)

per page

Available Filters

Product Type
  1. Lysate (1)
  2. Primary Antibody (2)
  3. Protein | Growth Factor (1)
Clonality
  1. Polyclonal (2)
Target
  1. ALPPL2 (3)
Reactivity
  1. Human (Hu) (2)
  2. Mouse (Ms) (1)
  3. Rat (Rt) (1)
Applications
  1. Paraffin Sections (P) (2)
  2. Enzyme Immunoassay (E) (1)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  4. Western blot / Immunoblot (WB) (1)
Presentation
  1. Aff - Purified (1)
  2. Purified (1)
Host
  1. Rabbit (2)
Images
  1. 1 image (3)
  2. 3+ images (1)
Images: Applications
  1. IHC (2)
  2. Immunofluorescence (IF) (1)
  3. Western blot / Immunoblot (WB) (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (4)
Service
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