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ALX3 antibody

Principal name

ALX3 antibody

Alternative names for ALX3 antibody

Homeobox protein aristaless-like 3, Proline-rich transcription factor ALX3

SwissProt ID

O70137 (Mouse), O95076 (Human)

Gene ID

257 (ALX3), 11694 (Alx3), 365900

Ncbi ID

9606, NP_031467, NP_006483.2, NM_006492, NP_006483, NP_031467.1

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), Por (Porcine), Rb (Rabbit), Bov (Bovine), Eq (Equine), GP (Guinea Pig)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of ALX3 antibody

Aristaless-related genes are a group of paired-related homeobox genes which play a role in regulating vertebrate embryogenesis. The homeodomain transciption factor aristaless-like 3 (ALX3) is expressed in mouse embryos from 8 days of gestation, predominantly in neural crest-derived mesenchyme and in lateral plate mesoderm. Expression analysis of human and mouse tissue reveals predominant ALX3 expression in brain tissue. The Alx3 gene maps to chromosome 1p23-p13 and encodes a 343 amino acid protein. Preferential methylation of Alx3 occurs in advanced-stage neuroblastoma and may repress ALX3 expression. Treatment with the methylation inhibitor 5-aza-2’-deoxycytidine restores ALX3 expression. Alx3 (-) mice lack a phenotype distinct from wild-type mice, however Alx3/Alx4 double mutants demonstrate severe craniofacial abnormalities not present in Alx4 single mutants. Specifically, Alx3/Alx4 double mutant newborn mice have cleft nasal regions in addition to malformation of other neural crest-derived skull structures.

General readings

Yamada,R., et al., (2004) Dev. Biol. 274 (2), 295-307

Mouse NIH-3T3; WB Suggested Anti-ALX3 Antibody Titration: 2.5ug/ml. ELISA Titer: 1:1562500. Positive Control: NIH/3T3 cell lysate; ALX3 antibody - N-terminal region (ARP37852_T100) in Mouse NIH-3T3 cells using Western Blot

ARP37852_T100

Western blot (WB) analysis of ALX3 antibody (Cat.-No.: AP06648PU-N) at 1/500 dilution
Lane1: Hela whole cell lysate
Lane2: NIH-3T3 whole cell lysate
Lane3: Rat brain tissue lysate

AP06648PU-N

Immunofluorescence analysis of HeLa cells, using ALX3 Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from 293 cells, using ALX3 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemical analysis of ALX3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of ALX3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of ALX3 expression in HeLa (A); NIH3T3 (B); rat brain (C) whole cell lysates.

TP317304

Western blot analysis of extracts from 293 cells, using ALX3 antibody.The lane on the right is treated with the synthesized peptide.

TA313448

Immunofluorescence analysis of HeLa cells, using ALX3 antibody.The picture on the right is treated with the synthesized peptide.

TA313448

WB Suggested Anti-Alx3 Antibody; Titration: 1.0 ug/ml; Positive Control: Rat Thymus

TA330586

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP317304	ALX3
	Recombinant protein of human ALX homeobox 3 (ALX3)
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

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