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ALX4 antibody

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Principal name

ALX4 antibody

Alternative names for ALX4 antibody

Homeobox protein aristaless-like 4, KIAA1788

SwissProt ID

O35137 (Mouse), Q4LAL6 (Bovin), Q9H161 (Human)

Gene ID

60529 (ALX4), 11695 (Alx4)

Ncbi ID

NP_068745.2, NP_068745, NM_007442, 9606, AAI66622.1, NM_021926, NP_031468.1, BC049786

Available reactivities

Ms (Mouse), Hu (Human), Can (Canine), Rt (Rat), Bov (Bovine), Chk (Chicken), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F), Enzyme Immunoassay (E)

Background of ALX4 antibody

This gene encodes a paired-like homeodomain transcription factor expressed inThe mesenchyme of developing bones, limbs, hair, teeth, and mammary tissue. Mutations inThis gene cause parietal forami 2 (PFM2); an autosomal domint disease characterized by deficient ossification ofThe parietal bones. Mutations inThis gene also cause a form of frontosal dysplasia with alopecia and hypogodism; suggesting a role forThis gene in craniofacial development, mesenchymal-epithelial communication, and hair follicle development. Deletion of a segment of chromosome 11 containingThis gene, del(11)(p11p12), causes Potocki-Shaffer syndrome (PSS); a syndrome characterized by craniofacial anomalies, mental retardation, multiple exostoses, and genital abnormalities in males. In mouse,This gene has been shown to use dual translation initiation sites located 16 codons apart. [provided by RefSeq, Oct 2009].

General readings

Wakui,K., et al., (2005) Eur. J. Hum. Genet. 13 (5), 528-540

AM05262PU-N - ALX4
Figure 1. Western Blot analysis of Alx-4 antibody (AM05262PU-N)
AP50156PU-N - ALX4
ALX4 Antibody (Center) (Cat. #AP50156PU-N) western blot analysis in MDA-MB435 cell line lysates (35µg/lane).This demonstrates the ALX4 antibody detected the ALX4 protein (arrow).
AP50156PU-N - ALX4
ALX4 Antibody (Center) (Cat. #AP50156PU-N) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ARP32356_P050 - ALX4
Human Jurkat; WB Suggested Anti-ALX4 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate; ALX4 antibody - N-terminal region (ARP32356_P050) in Human Jurkat cells using Western Blot
ARP33239_P050 - ALX4
Human Muscle; WB Suggested Anti-ALX4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Human Muscle; ALX4 antibody - middle region (ARP33239_P050) in Human Muscle cells using Western Blot
ARP33240_P050 - ALX4
Human Jurkat; WB Suggested Anti-ALX4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Jurkat cell lysate; ALX4 antibody - N-terminal region (ARP33240_P050) in Human Jurkat cells using Western Blot
ARP36700_T100 - ALX4
Human HepG2; WB Suggested Anti-ALX4 Antibody Titration: 0.625ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; ALX4 antibody - N-terminal region (ARP36700_T100) in Human HepG2 cells using Western Blot
ARP36701_P050 - ALX4
Human HepG2; WB Suggested Anti-ALX4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: HepG2 cell lysate; ALX4 antibody - C-terminal region (ARP36701_P050) in Human HepG2 cells using Western Blot
ARP36820_P050 - ALX4
Mouse Brain; WB Suggested Anti-Alx4 Antibody Titration: 0.2-1 ug/ml. Positive Control: Mouse Brain; Alx4 antibody - middle region (ARP36820_P050) in Mouse Brain cells using Western Blot
TA505173 - ALX4
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ALX4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505173)
TA505173 - ALX4
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ALX4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505173)
TA505173 - ALX4
Anti-ALX4 mouse monoclonal antibody (TA505173) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ALX4(RC224459).
TA505173 - ALX4
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALX4 (RC224459, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALX4.
TA505240 - ALX4
Anti-ALX4 mouse monoclonal antibody (TA505240) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ALX4(RC224459).
TA505240 - ALX4
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALX4 (RC224459, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALX4.
TA505145 - ALX4
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ALX4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505145)
TA505145 - ALX4
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALX4 (RC224459, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALX4.
TA505145 - ALX4
Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-ALX4 monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).
TA505146 - ALX4
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALX4 (RC224459, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALX4.
TA505146 - ALX4
HEK293T cells transfected with either RC224459 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ALX4 antibody(TA505146), and then analyzed by flow cytometry.
UM500083 - ALX4
Immunohistochemical staining of FFPE human breast tissue section using anti-ALX4 mouse monoclonal antibody UM500083, clone UMAB118 at 1:200. (Heat-induced epitope retrieval by Tris-EDTA, pH9.0; Polink1 Broad HRP for 15 min; DAB chromogen for 5 min & DAB enhancer for 30 sec).
UM500083 - ALX4
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-ALX4 mouse monoclonal antibody (UM500083). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
TA330266 - ALX4
WB Suggested Anti-ALX4 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate
TA331782 - ALX4
WB Suggested Anti-ALX4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: Human Muscle
TA331783 - ALX4
WB Suggested Anti-ALX4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: Jurkat cell lysate
TA341710 - ALX4
WB Suggested Anti-ALX4 Antibody Titration: 0.625 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate
TA341711 - ALX4
WB Suggested Anti-ALX4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: HepG2 cell lysate
TA341743 - ALX4
WB Suggested Anti-Alx4 Antibody Titration: 0.2-1 ug/ml; Positive Control: Mouse Brain
TA350626 - ALX4
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using (ALX4 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)
TA350626 - ALX4
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (ALX4 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)
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12 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ALX4 antibody
Figure 1. Western Blot analysis of Alx-4 antibody (AM05262PU-N) Mouse IgG1 KAbB4 Purified Ms WB
0.1 mg / €400.00
  OriGene Technologies GmbH
 

Published recently!

ALX4 antibody
  ALX4 mouse monoclonal antibody,clone UMAB118  
Immunohistochemical staining of FFPE human breast tissue section using anti-ALX4 mouse monoclonal antibody UM500083, clone UMAB118 at 1:200. (Heat-induced epitope retrieval by Tris-EDTA, pH9.0; Polink1 Broad HRP for 15 min; DAB chromogen for 5 min & DAB enhancer for 30 sec). Mouse IgG1 UMAB118 Purified Hu P
0.1 ml / €468.00
  OriGene Technologies, Inc.
+1 additional image

Highly validated antibody!

UltraMAB

ALX4 antibody
  ALX4 mouse monoclonal antibody,clone UMAB118  
Immunohistochemical staining of FFPE human breast tissue section using anti-ALX4 mouse monoclonal antibody UM500083, clone UMAB118 at 1:200. (Heat-induced epitope retrieval by Tris-EDTA, pH9.0; Polink1 Broad HRP for 15 min; DAB chromogen for 5 min & DAB enhancer for 30 sec). Mouse IgG1 UMAB118 Purified Hu P
30 µl / €180.00
  OriGene Technologies, Inc.
+1 additional image

Highly validated antibody!

UltraMAB

ALX4 antibody
  ALX4 mouse monoclonal antibody, clone OTI6B3 (formerly 6B3)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ALX4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505173) Mouse IgG1 OTI6B3 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.
+3 additional images

Highly validated antibody!

TrueMAB

ALX4 antibody
  Rabbit Polyclonal Anti-ALX4 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using (ALX4 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

ALX4 antibody
  ALX4 mouse monoclonal antibody, clone OTI1F2 (formerly 1F2)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ALX4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505145) Mouse IgG1 OTI1F2 Purified Hu P, WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
+2 additional images

TrueMAB

ALX4 antibody
  ALX4 mouse monoclonal antibody, clone OTI2F2 (formerly 2F2)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALX4 (RC224459, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALX4. Mouse IgG1 OTI2F2 Purified Hu F, WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
+1 additional image

TrueMAB

ALX4 antibody
  ALX4 mouse monoclonal antibody, clone OTI2E1 (formerly 2E1)  
Anti-ALX4 mouse monoclonal antibody (TA505240) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ALX4(RC224459). Mouse IgG1 OTI2E1 Purified Hu ICC/IF, WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
+1 additional image

TrueMAB

ALX4 (Center) antibody
ALX4 Antibody (Center) (Cat. #AP50156PU-N) western blot analysis in MDA-MB435 cell line lysates (35µg/lane).This demonstrates the ALX4 antibody detected the ALX4 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

Lysates

Catalog No.    

ALX4 overexpression lysate
ALX4 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Alx4 (GFP-tagged) - Mouse aristaless 4 (cDNA clone MGC:59384 IMAGE:6506755), 200 uL, >10^7 TU/mL
Lenti ORF particles, Alx4 (GFP-tagged) - Mouse aristaless 4 (cDNA clone MGC:59384 IMAGE:6506755), 200 uL, >10^7 TU/mL  
200 µl / €1,240.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Alx4 (Myc-DDK-tagged) - Mouse aristaless 4 (cDNA clone MGC:59384 IMAGE:6506755), 200 uL, >10^7 TU/mL
Lenti ORF particles, Alx4 (Myc-DDK-tagged) - Mouse aristaless 4 (cDNA clone MGC:59384 IMAGE:6506755), 200 uL, >10^7 TU/mL  
200 µl / €1,110.00
  OriGene Technologies, Inc.
 

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  2. Mouse (Ms) (2)
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  2. Paraffin Sections (P) (5)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (2)
  4. Enzyme Immunoassay (E) (1)
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