Aminoacylase-2 / ACY2 antibody
Principal name
Aminoacylase-2 / ACY2 antibody
Alternative names for Aminoacylase-2 / ACY2 antibody
Aspartoacylase, ASPA, ACY-2, ASP
Ncbi ID
9606, NP_000040, NP_000040.1, NP_001121557, NP_075602.2, NM_024399, NM_000049, NM_001128085
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)
Background of Aminoacylase-2 / ACY2 antibody
- Kit Component:
- - KN206564G1, ASPA gRNA vector 1 in pCas-Guide vector
- KN206564G2, ASPA gRNA vector 2 in pCas-Guide vector
- KN206564D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Le Coq. et al. (2006) Biochemistry 45: 5878-5884.Le Coq. et al. (2008) Biochemistry 47: 3484-3492.
Detection limit for recombinant GST tagged ASPA is approximately 1ng/ml as a capture antibody.
Western Blot analysis of ASPA expression in transfected 293T cell line (H00000443-T01) by ASPA MaxPab polyclonal antibody.Lane 1: ASPA transfected lysate(34.43 KDa).Lane 2: Non-transfected lysate.
ASPA MaxPab rabbit polyclonal antibody. Western Blot analysis of ASPA expression in mouse liver.
ASPA MaxPab rabbit polyclonal antibody. Western Blot analysis of ASPA expression in human liver.
Western Blot analysis of ASPA expression in transfected 293T cell line (H00000443-T01) by ASPA MaxPab polyclonal antibody.Lane 1: ASPA transfected lysate(35.70 KDa).Lane 2: Non-transfected lysate.
ASPA MaxPab rabbit polyclonal antibody. Western Blot analysis of ASPA expression in mouse liver.
ASPA MaxPab rabbit polyclonal antibody. Western Blot analysis of ASPA expression in human liver.
Western Blot analysis of ASPA expression in transfected 293T cell line (H00000443-T01) by ASPA MaxPab polyclonal antibody.Lane 1: ASPA transfected lysate(35.70 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation of ASPA transfected lysate using anti-ASPA MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ASPA MaxPab mouse polyclonal antibody (B01) (H00000443-B01).
Human kidney; WB Suggested Anti-ASPA Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human kidney; ASPA antibody - N-terminal region (ARP56072_P050) in Human kidney cells using Western Blot
Human HepG2; WB Suggested Anti-ASPA Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; ASPA antibody - middle region (ARP56073_P050) in Human HepG2 cells using Western Blot
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ASPA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805223)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASPA (RC206564, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ASPA.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASPA (RC206564, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ASPA.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ASPA mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA805304)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ASPA mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA805304)(1:150)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ASPA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA805304)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ASPA mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA805304)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASPA (RC206564, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ASPA.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASPA (RC206564, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ASPA.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ASPA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805325)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ASPA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805325)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASPA (RC206564, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ASPA.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ASPA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805327)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ASPA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805327)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ASPA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805327)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASPA (RC206564, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ASPA.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASPA (RC206564, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ASPA.(1:2000)
Sample (30 ug of whole cell lysate). A: Molt-4 . B: Raji. 10% SDS PAGE. TA308661 diluted at 1:1000.
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Aspartoacylase(TA308661) antibody at 1:100 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Aspartoacylase(TA308661) antibody at 1:200 dilution.
(0.01ug/ml) staining of Mouse Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of ASPA Antibody (N-term) (Cat. #TA324873) in MCF7 cell line lysates (35ug/lane). ASPA (arrow) was detected using the purified Pab.
Western blot analysis of ASPA (arrow) using rabbit polyclonal ASPA Antibody (N-term) (Cat. #TA324873). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ASPA gene.
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ASPA Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of ASPA Antibody (N-term)(Cat. #TA324873) with MCF-7 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of MDA-MB468 cells using ASPA Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
WB Suggested Anti-ASPA Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human kidney
WB Suggested Anti-ASPA Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate
- Quick-Filters:
- Highly Validated (3)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA324873 | Aminoacylase-2 / ACY2 (N-term) antibody |
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| Rabbit polyclonal ASPA Antibody (N-term) | |||||||
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Rabbit | Hu | F, ICC/IF, P, WB |
0.4 ml /
€415.00
|
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| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody! |
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| TA805304 | Aminoacylase-2 / ACY2 antibody |
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| ASPA mouse monoclonal antibody, clone OTI3G5 (formerly 3G5) | |||||||
 |
Mouse | IgG1 | OTI3G5 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA805327 | Aminoacylase-2 / ACY2 antibody |
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| ASPA mouse monoclonal antibody, clone OTI2D2 (formerly 2D2) | |||||||
 |
Mouse | IgG2a | OTI2D2 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA308661 | Aminoacylase-2 / ACY2 antibody |
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| Rabbit Polyclonal antibody to Aspartoacylase (aspartoacylase (Canavan disease)) | |||||||
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Rabbit | IgG | Aff - Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA311514 | Aminoacylase-2 / ACY2 (C-term) antibody |
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| Goat Anti-Aspa (mouse) Antibody | |||||||
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Goat | Aff - Purified | Ms, Rt | E, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA805223 | Aminoacylase-2 / ACY2 antibody |
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| ASPA mouse monoclonal antibody, clone OTI5B8 (formerly 5B8) | |||||||
 |
Mouse | IgG1 | OTI5B8 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA805303 | Aminoacylase-2 / ACY2 antibody |
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| ASPA mouse monoclonal antibody, clone OTI3G7 (formerly 3G7) | |||||||
 |
Mouse | IgG2b | OTI3G7 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA805324 | Aminoacylase-2 / ACY2 antibody |
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| ASPA mouse monoclonal antibody, clone OTI4H4 (formerly 4H4) | |||||||
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Mouse | IgG2a | OTI4H4 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA805325 | Aminoacylase-2 / ACY2 antibody |
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| ASPA mouse monoclonal antibody, clone OTI1A11 (formerly 1A11) | |||||||
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Mouse | IgG2a | OTI1A11 | Purified | Hu | P |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA805326 | Aminoacylase-2 / ACY2 antibody |
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| ASPA mouse monoclonal antibody, clone OTI5C7 (formerly 5C7) | |||||||
 |
Mouse | IgG1 | OTI5C7 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA805343 | Aminoacylase-2 / ACY2 antibody |
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| ASPA mouse monoclonal antibody, clone OTI3B5 (formerly 3B5) | |||||||
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Mouse | IgG2a | OTI3B5 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP306564 | Aminoacylase-2 / ACY2 (transcript variant 1) |
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| Recombinant protein of human aspartoacylase (Canavan disease) (ASPA), transcript variant 1 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP325443 | Aminoacylase-2 / ACY2 (transcript variant 2) |
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| Recombinant protein of human aspartoacylase (Canavan disease) (ASPA), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR207562L2V | Lenti ORF particles, Aspa (GFP-tagged ORF) - Rat aspartoacylase (Aspa), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR207562L1V | Lenti ORF particles, Aspa (Myc-DDK-tagged ORF) - Rat aspartoacylase (Aspa), 200 uL, >10^7 TU/mL |
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200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||