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Amyloid beta antibody

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Principal name

Amyloid beta antibody

Alternative names for Amyloid beta antibody

Amyloid beta peptide

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Bov (Bovine), Sh (Sheep), Prim (Primate)

Available hosts

Rabbit, Mouse, Chicken

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (capture) (E(capture)), Dot blot (Dot), Radioimmunoassay (R), ELISA (detection) (E(detection))

Background of Amyloid beta antibody

Amyloid beta precursor protein gene (ABPP) encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Multiple transcript variants encoding several different isoforms have been found for this gene. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751 and isoform APP770 are widely expressed in nonneuronal cells. Isoform APP751 is the most abundant form in T lymphocytes. ABPP is expressed in all fetal tissues examined with the highest levels in brain, kidney, heart and spleen with weak expression observed in liver; ABPP is induced during neuronal differentiation. In the adult brain, highest expression of ABPP gene is found in the frontal lobe of the cortex and in the anterior perisylvian cortex opercular gyri; moderate expression in the cerebellar cortex, the posterior perisylvian cortex opercular gyri and the temporal associated cortex. Weak expression is found in the striate, extra striate and motor cortices. Mutations in ABPP have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy).

General readings

1. N-terminal EFRH sequence of Alzheimer's beta-amyloid peptide represents the epitope of its anti-aggregating antibodies. (Frenkel D; J Neuroimmunol, 1998 Aug 1)
2. Activation of nuclear factor-kappa B by beta-amyloid peptides and interferon-gamma in murine microglia. (Bonaiuto C; J Neuroimmunol, 1997 Jul) (Luo Y; Neurosci Lett, 1996 Oct 18)
3. Alzheimer's beta-amyloid peptides induce inflammatory cascade in human vascular cells: the roles of cytokines and CD40. (Suo Z; Brain Res, 1998 Oct 5)
4. Amyloid beta peptide of Alzheimer's disease downregulates Bcl-2 and upregulates bax expression in human neurons. (Paradis E; J Neurosci, 1996 Dec 1)
5. Nontoxic amyloid beta peptide 1-42 suppresses acetylcholine synthesis. Possible role in cholinergic dysfunction in Alzheimer's disease. (Hoshi M; J Biol Chem, 1997 Jan 24)
6.Transcription factor NF-kappaB is activated in primary neurons by amyloid beta peptides and in neurons surrounding early plaques from patients with Alzheimer disease. (Kaltschmidt B; Proc Natl Acad Sci U S A, 1997
Mar 18)
7. Disaggregation of Alzheimer beta-amyloid by site-directed mAb. (Solomon B; Proc Natl Acad Sci U S A, 1997 Apr 15)

AM00001BT-N - Amyloid beta
Immunoblot Analysis: Amyloid beta A4 peptides were applied on SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with 2µg/ml mab bA4 (40)-5C3 for 1h at 15-22°C and developed by ECL (exposure time: 30 sec). Lane 1: bA4 (1-40)Lane 2: bA4 (1-42)Lane 3: bA4 (1-43)
AM00001PU-N - Amyloid beta
Immunoblot Analysis: Amyloid beta A4 peptides were applied on SDS-PAGE and transferred to a PVDF membrane. The Immunoblot was probed with 2 µg/ml mab bA4 (40)-5C3 for 1h at 15-22°C and developed by ECL (exposure time: 30 sec). Lane 1: bA4 (1-40), Lane 2: bA4 (1-42), Lane 3: bA4 (1-43)
AM00003BT-N - Amyloid beta
Immunoblot Analysis: Amyloid beta A4 peptides were applied on SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with 2µg/ml mab bA4 (42)-8G7 for 1h at 15-22°C and developed by ECL (exposure time: 30 sec).Lane 1: bA4(1-40) Lane 2: bA4 (1-42) Lane 3: bA4 (1-43)
AM00003PU-N - Amyloid beta
Figure 1. Immunoblot Analysis Amyloid beta A4 peptides: Lane 1: bA4(1-40), Lane 2: bA4 (1-42), Lane 3: bA4 (1-43) were applied on SDS-PAGE and transferred to a PVDF membrane. The Immunoblot was probed with 2 µg/ml mab bA4 (42)-8G7for 1h at 15-22°C and developed by ECL (exposure time: 30 sec).
AM00004PU-N - Amyloid beta
Immunoblot Analysis: Amyloid beta A4 peptides (lane 1: bA4(1-40); lane 2: bA4 (1-42); lane 3: bA4 (1-43)) were applied on SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with 2 µg/ml AM00004PU-N for 1h at 15-22°C and developed by ECL (exposure time: 30 sec).
AM00006PU-N - Amyloid beta
Immunoblot Analysis: Amyloid beta A4 peptides (lane 1: bA4(1-40); lane 2: bA4 (1-42); lane 3: bA4 (1-43)) were applied on SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with 2µg/ml mab bA4N-19H5 for 1h at 15-22°C and developed by ECL (exposure time: 30 sec).
NB300-225 - Amyloid beta
Western Blot: Abeta 40 Antibody [NB300-225] - Detection of Abeta 40 on 5 ng of peptide per lane using NB 300-225. Lane 1: Abeta-40, lane 2: Abeta-42, Lane 3: Abeta-40 and -42 mix.
NB300-226 - Amyloid beta
Detection of Abeta 42 on 5 ng of peptide per lane using NB 300-226 (lot A). Lane 1: Abeta-40, lane 2: Abeta-42, lane 3: Abeta-40 and -42 mix. Band appears at approximately 6 kDa.
GTX40068 - Amyloid beta
Amyloid beta A4 peptides were applied to SDS-PAGE and transferred to a PVDF membrane. The blot was probed with 2 μg/mL GTX40068 for 1 hour at 15-22ºC and developed by ECL (exp. time: 30 sec.). x000DLane 1: bA4 (1-40) x000DLane 2: bA4 (1-42) x000DLane 3: bA4 (1-43)
AP09220PU-N - Amyloid beta
AP09220PU-N Beta Amyloid antibody staining of Formalin-Fixed, Paraffin-Embedded Human Heart at 5 µg/ml followed by biotinylated Goat anti-Rabbit IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen.
AP09220PU-N - Amyloid beta
AP09220PU-N Beta Amyloid antibody staining of TG APP23 Mouse brain cortex Frozen Sections. Anti-Beta Amyloid Antibody used at 1/200 and incubated for 2 hours in TBS/BSA/Tween/Azide. Fluorescent labelled anti-Rabbit IgG was then added. Carl Hobbs, King's College London, United Kingdom.
AP09220PU-N - Amyloid beta
Western blot analysis using AP09220PU-N Beta Amyloid antibody in Mouse Brain (Lane 1) and Mouse Spinal Chord (Lane 2).4-20% gradient gel, Blocked in 1% BSA-TBS-T 30min RT and probed with Rb-a-Beta Amyloid 1:1000 in 1% BSA-TBS-T o/n 4°C. HRP Goat-anti-Rabbit R1454HRP (Lot#21231) 1/40,000 in MB-070 30 min RT. FEMTOMAX chemiluminescent substrate was used for detection of a 40-50 kD band consistent with a higher MW precursor form of beta amyloid. A secondary Ab only control showed no detectable signal.
MAB2675 - Amyloid beta
Immunoblot analysis of cell lysates.Lane 1 : Bosc23 cell lysate.Lane 2 : Bosc23 cell lysate transfected with GFP.Lane 3 : Bosc23 cell lysate transfected with GFP-Amyloid beta 40.Lane 4 : Bosc23 cell lysate transfected with GFP-Amyloid beta 42.
MAB3915 - Amyloid beta
Western blot analysis of Beta amyloid peptides were applied to SDS-PAGE and transferred to a PVDF membrane.The blot was probed with 2µg/ml Beta amyloid monoclonal antibody, clone BDI780 ( Cat # MAB3915 ) for 1 hour at 15-22°C and developed by ECL.Lane 1 : Beta amyloid ( 1-40 ).Lane 2 : Beta amyloid ( 1-42 ).Lane 3 : Beta amyloid ( 1-43 ).
MAB3916 - Amyloid beta
Western blot analysis of Beta amyloid peptides were applied to SDS-PAGE and transferred to a PVDF membrane.The blot was probed with 2µg/ml Beta amyloid monoclonal antibody, clone BDI260 ( Cat # MAB3916 ) for 1 hour at 15-22°C and developed by ECL.Lane 1 : Beta amyloid ( 1-40 ).Lane 2 : Beta amyloid ( 1-42 ).Lane 3 : Beta amyloid ( 1-43 ).
MAB3921 - Amyloid beta
Western blot analysis of Beta amyloid peptides were applied to SDS-PAGE and transferred to a PVDF membrane.The blot was probed with 2µg/ml Beta amyloid monoclonal antibody, clone BDI590 ( Cat # MAB3921 ) for 1 hour at 15-22°C and developed by ECL.Lane 1 : Beta amyloid ( 1-40 ).Lane 2 : Beta amyloid ( 1-42 ).Lane 3 : Beta amyloid ( 1-43 ).
PAB13587 - Amyloid beta
Dot blot of Beta amyloid polyclonal antibody ( Cat # PAB13588 ) at a 1 : 1000 dilution. The antibody can detect Beta amyloid ( 1-42 ), Beta amyloid ( 1-28 ), Beta amyloid ( 1-20 ) and Beta amyloid ( 1-17 ).
PAB13588 - Amyloid beta
Western blot analysis of Beta amyloid ( 1-42 ) with Beta amyloid polyclonal antibody ( Cat # PAB13588 ) at a 1 : 1000 dilution.
PAB13588 - Amyloid beta
Dot blot of Beta amyloid polyclonal antibody ( Cat # PAB13588 ) at a 1 : 1000 dilution. The antibody can detect Beta amyloid ( 1-42 ), Beta amyloid ( 1-28 ), Beta amyloid ( 1-20 ) and Beta amyloid ( 1-17 ).
NBP1-44047 - Amyloid beta
Western Blot: Abeta 40 Antibody [NBP1-44047] - 1-40(lane 1) and 1-42 (lane 2) ABeta peptides were run on a 16.5% TRIS/tricine gel and transferred to PVDF membrane. The peptide concentration was approximately 0.5ng/lane. The blot was incubated with affinity purified AB40 antibody at 1ug/ml, followed by a oat and rabbit –HRP antibody at 1:40,000 and developed using a standard chemiluninescence method. A single band is present only in lane 1 which contains the ABeta 1-40 peptide and no signal is found in lane 2 which contains the ABeta 1-42 peptide.
NBP1-44048 - Amyloid beta
Immunofluorescence: beta Amyloid [Pyroglutamate-3] Antibody [NBP1-44048] - Laser scanning confocal microscopy was carried out using human tissue sections from frontal cortex. Figure A shows an example section that was immunostained for the presence of pyrE3Abeta (green). Figure B shows that the immunogen peptide completely blocked the NBP1-44048 antibody.
NBP1-44048 - Amyloid beta
Western Blot: beta Amyloid Antibody [NBP1-44048] - 3-42 and pyrE3-42 ABeta peptides were run on a 16.5% TRIS/tricine gel and transferred to PVDF membrane. The peptide concentration was approximately 0.5ug/lane. Cross reactivity with E3-42 ABeta peptide is >1000 fold below pyrE3-42 reactivity (data not shown) and contains no reactivity towards the 1-42 ABeta peptide (data not shown).
NBP1-44070 - Amyloid beta
Immunofluorescence: beta Amyloid Antibody [NBP1-44070] - Laser scanning confocal microscopy was carried out using human tissue sections from frontal cortex. Figure A shows an example section that was immunostained for the presence of pyrE11Abeta (green). Figure B shows that the immunogen peptide completely blocked the NBP1-44070 antibody.
AP31802PU-N - Amyloid beta
Beta-Amyloid-positive “neuritic plaque” in cerebral cortex as seen in a post-mortum specimen taken from an Alzheimer's disease patient. Picture courtesy of Dr. Randy Woltjer, Oregon Health & Sciences University.
NBP1-78007 - Amyloid beta
Immunohistochemistry-Paraffin: beta Amyloid Antibody [NBP1-78007] - Human Heart (formalin-fixed, paraffin-embedded) stained with Anti-Beta Amyloid Antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
NBP1-78007 - Amyloid beta
Immunohistochemistry-Paraffin: beta Amyloid Antibody [NBP1-78007] - human, multi-brain and multi-cancer slides. Within the multi-tumor block, the antibody showed variable levels of nuclear and cytoplasmic staining between individual tumors, with some tumors showing moderate staining. This image shows EGR-1 staining of human ovarian carcinoma.
NBP1-78007 - Amyloid beta
Immunofluorescence: beta Amyloid Antibody [NBP1-78007] - Immunohistochemistical detection of beta Amyloid using Anti-Beta Amyloid Antibody on TG APP23 mouse brain cortex frozen sections. Anti-Beta Amyloid Antibody used at 1/200 and incubated for 2 hours in TBS/BSA/Tween/azide. Fluorescent labelled anti rabbit IgG was then added. _x000d_Carl Hobbs, King`s College London, United Kingdom
NBP1-78007 - Amyloid beta
Western Blot: beta Amyloid Antibody [NBP1-78007] - ing to EGR-1 present in mouse embryonic fibroblast whole cell lysate (arrowhead). Approximately 35 ug of lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500. Reaction occurred 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of Dye 800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
NBP1-78007 - Amyloid beta
Western Blot: beta Amyloid Antibody [NBP1-78007] - Mouse Brain (Lane 1) and Mouse Spinal Chord (Lane 2) were run on a 4-20% gradient gel, Blocked in 1% BSA-TBS-T 30 min RT and probed with Rb-a-Beta Amyloid 1:1000 in 1% BSA-TBS-T o/n 4°C.
AM26393PU-L - Amyloid beta
Detection of Aβ42 on 10 ng /dot of Aβ40 or Aβ42 using Clone CA9 3B3 at 1µg/ml.
AM26394PU-L - Amyloid beta
Detection of Abeta 42 peptide using antibody Clone 10C11 at 2 µg/ml dilution
AM26394PU-L - Amyloid beta
Detection of Abeta 42 on 10 ng of peptide per lane using antibody Clone CA9 10C11 at 1 µg/ml dilution.
AM26395PU-L - Amyloid beta
Immunohistochemistry experiment using NT-3F5 (1/100) on the brain tissue from transgenic Alzheimer's disease mouse model. The plaques showed strong positive signal and the background was clear.
AM26415PU-L - Amyloid beta
Ascites (5B8) at 1:5000 dilution
AM26686AF-N - Amyloid beta
Paraffin section of Alzheimer’s disease brain stained with AM26686AF-N
AM26686AF-N - Amyloid beta
Western blot analysis of A-beta 1-40 (1) and A-beta 1-42 (2) using AM26686AF-N
CH23020-100 - Amyloid beta
beta-Amyloid staining through a cross section of a human Alzheimer’s brain. Note staining of "neuritic plaques," which include both the beta-amyloid plaques plus the residue of dead neurons.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Amyloid beta (1-40/42) antibody
Amyloid beta Mouse IgG1 9F1 FITC Hu ICC/IF
0.1 mg / €450.00
  OriGene Technologies GmbH

Amyloid beta (1-42 specific) antibody
Figure 1. Immunoblot Analysis Amyloid beta A4 peptides: Lane 1: bA4(1-40), Lane 2: bA4 (1-42), Lane 3: bA4 (1-43) were applied on SDS-PAGE and transferred to a PVDF membrane. The Immunoblot was probed with 2 µg/ml mab bA4 (42)-8G7for 1h at 15-22°C and developed by ECL (exposure time: 30 sec). Mouse IgG1 8G7 Purified Hu E, ICC/IF, WB
0.1 mg / €400.00
  OriGene Technologies GmbH

Amyloid beta (1-40) antibody
Amyloid beta Mouse IgG1 AP-5 Aff - Purified Hu IP, P
0.1 mg / €390.00
  OriGene Technologies GmbH

Amyloid beta (1-40 specific) antibody
Amyloid beta Mouse IgG1 CV9 7B10 Biotin Hu E, E(detection)
0.1 ml / €510.00
  OriGene Technologies GmbH

Amyloid beta (1-40 specific) antibody
Amyloid beta Mouse IgG1 CV9 7B10 Aff - Purified Hu E, E(detection)
0.5 mg / €470.00
  OriGene Technologies GmbH

Amyloid beta (1-40 specific) antibody
Amyloid beta Mouse IgG1 CV9 8B2 Aff - Purified Hu E
0.5 mg / €470.00
  OriGene Technologies GmbH

Amyloid beta (1-42 specific) antibody
Amyloid beta Mouse IgG1 Biotin Hu E, E(detection)
0.1 ml / €510.00
  OriGene Technologies GmbH

Amyloid beta (1-42 specific) antibody
Detection of Aβ42 on 10 ng /dot of Aβ40 or Aβ42 using Clone CA9 3B3 at 1µg/ml. Mouse IgG2b CA9 3B3 Aff - Purified Hu, Ms, Prim, Rt E, WB
0.5 mg / €470.00
  OriGene Technologies GmbH

Amyloid beta (1-42 specific) antibody
Detection of Abeta 42 peptide using antibody Clone 10C11 at 2 µg/ml dilution Mouse IgG2b CA9 10C11 Aff - Purified Hu, Ms, Prim, Rt E, E(detection), WB
0.5 mg / €470.00
  OriGene Technologies GmbH
+1 additional image

Amyloid beta (N-term) antibody
Immunohistochemistry experiment using NT-3F5 (1/100) on the brain tissue from transgenic Alzheimer's disease mouse model. The plaques showed strong positive signal and the background was clear. Mouse IgG2b NT 3F5 Aff - Purified Hu C
0.5 mg / €470.00
  OriGene Technologies GmbH

Amyloid beta (N-term) antibody
Ascites (5B8) at 1:5000 dilution Mouse IgG1 NT 5B8 Aff - Purified Hu E, WB
0.5 mg / €470.00
  OriGene Technologies GmbH

Amyloid beta (N-term) antibody
Paraffin section of Alzheimer’s disease brain stained with AM26686AF-N Mouse IgG2b 2C8 Azide Free Hu, Ms P, WB
0.1 mg / €425.00
  OriGene Technologies GmbH
+1 additional image

Amyloid beta (1-14) antibody
AP09220PU-N Beta Amyloid antibody staining of Formalin-Fixed, Paraffin-Embedded Human Heart at 5 µg/ml followed by biotinylated Goat anti-Rabbit IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €515.00
  OriGene Technologies GmbH
+2 additional images

Amyloid beta (N-term) antibody
Beta-Amyloid-positive “neuritic plaque” in cerebral cortex as seen in a post-mortum specimen taken from an Alzheimer's disease patient. Picture courtesy of Dr. Randy Woltjer, Oregon Health & Sciences University. Chicken Ig Aff - Purified Hu, Ms ICC/IF, P
0.1 mg / €550.00
  OriGene Technologies GmbH

Amyloid beta (1-40) antibody
Amyloid beta Mouse IgG1 BAM-10 Ascites Hu C, P
1 ml / €800.00
  OriGene Technologies GmbH

Amyloid beta (1-40) antibody
Amyloid beta Mouse IgG1 BAM-10 Ascites Hu C, P
0.5 ml / €520.00
  OriGene Technologies GmbH

Amyloid beta (1-17) antibody
Amyloid beta Mouse IgG1 DE2B4 Purified Hu E, IP, P
0.1 mg / €330.00
  OriGene Technologies GmbH

Amyloid beta (1-40) antibody
Amyloid beta Rabbit Serum Hu E, P, WB
0.1 ml / €590.00
  OriGene Technologies GmbH

Amyloid beta (1-42) antibody
Amyloid beta Rabbit Serum Hu E, P, WB
0.1 ml / €590.00
  OriGene Technologies GmbH

Amyloid beta (N-term) antibody
beta-Amyloid staining through a cross section of a human Alzheimer’s brain. Note staining of "neuritic plaques," which include both the beta-amyloid plaques plus the residue of dead neurons. Chicken Ig Aff - Purified Hu, Ms P
0.1 ml / €210.00
  Neuromics Antibodies
+1 additional image

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21 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. Primary Antibody (21)
Clonality
  1. Monoclonal (15)
  2. Polyclonal (6)
Target
  1. Amyloid beta (21)
Property
  1. 1-14 (1)
  2. 1-17 (1)
  3. 1-40 (5)
  4. 1-40 specific (3)
  5. 1-40/42 (1)
  6. 1-42 (1)
  7. 1-42 specific (4)
  8. N-term (5)
Reactivity
  1. Human (Hu) (21)
  2. Mouse (Ms) (7)
  3. Primate (Prim) (2)
  4. Rat (Rt) (2)
Applications
  1. Enzyme Immunoassay (E) (12)
  2. Paraffin Sections (P) (10)
  3. Western blot / Immunoblot (WB) (9)
  4. ELISA (detection) (E(detection)) (4)
  5. Immunocytochemistry/​Immunofluorescence (ICC/IF) (4)
  6. Frozen sections (C) (3)
  7. Immunoprecipitation (IP) (3)
Presentation
  1. Aff - Purified (10)
  2. Serum (3)
  3. Ascites (2)
  4. Biotin (2)
  5. Purified (2)
  6. Azide Free (1)
  7. FITC (1)
Host
  1. Mouse (15)
  2. Rabbit (4)
  3. Chicken (2)
Images
  1. 1 image (10)
  2. 3+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (5)
  2. Paraffin Sections (P) (3)
  3. Dot blot (Dot) (2)
  4. Frozen sections (C) (1)
  5. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Manufacturer
  1. OriGene Technologies (19)
  2. Neuromics Antibodies (2)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
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