ANKRA2 antibody
Principal name
ANKRA2 antibody
Alternative names for ANKRA2 antibody
RFXANK-like 2, Ankyrin repeat family A protein 2
Ncbi ID
9606, NP_075526, NP_075526.1, NP_075961.1, BC066113, BC079615, NM_023472
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP), Paraffin Sections (P)
Background of ANKRA2 antibody
- Kit Component:
- - KN301261G1, Ankra2 gRNA vector 1 in pCas-Guide vector
- KN301261G2, Ankra2 gRNA vector 2 in pCas-Guide vector
- KN301261D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
"Characterization of ANKRA, a novel ankyrin repeat protein that interacts with the cytoplasmic domain of megalin." Rader K., Orlando R.A., Lou X., Farquhar M.G. J. Am. Soc. Nephrol. 11:2167-2178(2000)
Immunofluorescence of monoclonal antibody to ANKRA2 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of ANKRA2 expression in transfected 293T cell line by ANKRA2 monoclonal antibody (M01), clone 1D11.Lane 1: ANKRA2 transfected lysate(34 KDa).Lane 2: Non-transfected lysate.
ANKRA2 monoclonal antibody (M01), clone 1D11 Western Blot analysis of ANKRA2 expression in IMR-32 ( Cat # L008V1 ).
Detection limit for recombinant GST tagged ANKRA2 is approximately 0.03ng/ml as a capture antibody.
Western Blot analysis of ANKRA2 expression in transfected 293T cell line (H00057763-T01) by ANKRA2 MaxPab polyclonal antibody.Lane 1: ANKRA2 transfected lysate(34.43 KDa).Lane 2: Non-transfected lysate.
ANKRA2 MaxPab rabbit polyclonal antibody. Western Blot analysis of ANKRA2 expression in mouse intestine.
Western Blot analysis of ANKRA2 expression in transfected 293T cell line (H00057763-T02) by ANKRA2 MaxPab polyclonal antibody.Lane 1: ANKRA2 transfected lysate(34.30 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of ANKRA2 expression in transfected 293T cell line (H00057763-T01) by ANKRA2 MaxPab polyclonal antibody.Lane 1: ANKRA2 transfected lysate(34.43 KDa).Lane 2: Non-transfected lysate.
Human HeLa; WB Suggested Anti-ANKRA2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Hela cell lysate; ANKRA2 antibody - C-terminal region (ARP50675_P050) in Human HeLa cells using Western Blot
ANKRA2 MaxPab rabbit polyclonal antibody. Western Blot analysis of ANKRA2 expression in mouse intestine.
Immunoprecipitation of ANKRA2 transfected lysate using anti-ANKRA2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ANKRA2 MaxPab mouse polyclonal antibody (B01) (H00057763-B01).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANKRA2 (RC204384, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANKRA2.(1:2000)
Predicted cell location: Cytoplasm. Positive control: Human colon and thyroid cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using ANKRA2 antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x 200)
Predicted cell location: Cytoplasm. Positive control: Human colon and thyroid cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using ANKRA2 antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)
Predicted cell location: Cytoplasm. Positive control: Human colon and lung cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using ANKRA2 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x 200)
Predicted cell location: Cytoplasm. Positive control: Human colon and lung cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using ANKRA2 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x200)
WB Suggested Anti-Ankra2 Antibody; Titration: 1.0 ug/ml; Positive Control: Mouse Testis
WB Suggested Anti-ANKRA2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Hela cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA322190 | ANKRA2 antibody |
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| Anti-ANKRA2 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA322191 | ANKRA2 antibody |
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| Anti-ANKRA2 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA808378 | ANKRA2 antibody |
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| ANKRA2 mouse monoclonal antibody, clone OTI8A9 (formerly 8A9) | |||||||
 |
Mouse | IgG1 | OTI8A9 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760098 | ANKRA2 (full length, N-term HIS tag) |
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| Recombinant protein of human ankyrin repeat, family A (RFXANK-like), 2 (ANKRA2), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
