AOF1 antibody
Principal name
AOF1 antibody
Alternative names for AOF1 antibody
Flavin-containing amine oxidase domain-containing protein 1
SwissProt ID
Q8NB78 (Human)
Gene ID
221656 (KDM1B)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB)
Background of AOF1 antibody
- Kit Component:
- - KN221980G1, KDM1B gRNA vector 1 in pCas-Guide vector
- KN221980G2, KDM1B gRNA vector 2 in pCas-Guide vector
- KN221980D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western blot analysis of KDM1B in 3T3 cell lysate with KDM1B antibody at 2 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Host: Rabbit; Target Name: KDM1B; Sample Tissue: A549 Whole Cell lysates; Antibody Dilution: 1.0ug/ml
WB using the antibody against KDM1B diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size 92 kDa) is indicated on the right; the marker (in kDa) is shown on the left.
Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against human KDM1B. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:14,250.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA326673 | AOF1 antibody |
||||||
| Rabbit Polyclonal KDM1B Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN221980 | KDM1B - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN221980BN | KDM1B - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN221980LP | KDM1B - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN221980RB | KDM1B - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Lysates
| Catalog No. | ||
|---|---|---|
| LY403510 | KDM1B overexpression lysate |
|
|
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |