APPBP1 antibody
Principal name
APPBP1 antibody
Alternative names for APPBP1 antibody
NAE1, HPP1, Amyloid protein-binding protein 1, Amyloid beta precursor protein-binding protein 1
Ncbi ID
9606, NP_001018169, NP_001018169.1, NP_003896.1, NM_003905, NM_001018160, NP_001018170, NM_032072, NM_001018159
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP)
Background of APPBP1 antibody
- Kit Component:
- - KN201326G1, NAE1 gRNA vector 1 in pCas-Guide vector
- KN201326G2, NAE1 gRNA vector 2 in pCas-Guide vector
- KN201326D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Jung, A.C., et al. Int. J. Cancer 126(8):1882-1894(2010)
Park, Y., et al. Biochem. Biophys. Res. Commun. 374(2):294-298(2008)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007)
Li, T., et al. Arch. Biochem. Biophys. 453(1):70-74(2006)
Norman, J.A., et al. Biochemistry 45(9):3014-3019(2006)
APPBP1 monoclonal antibody (M01), clone 4E8-H3 Western Blot analysis of APPBP1 expression in HeLa ( Cat # L013V1 ).
APPBP1 monoclonal antibody (M01), clone 4E8-H3. Western Blot analysis of APPBP1 expression in A-431 ( Cat # L015V1 ).
Detection limit for recombinant GST tagged APPBP1 is approximately 0.3ng/ml as a capture antibody.
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with NAE1 polyclonal antibody ( Cat # PAB2845 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of NAE1 ( arrow ) using rabbit NAE1 polyclonal antibody ( Cat # PAB2845 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the NAE1 gene ( Lane 2 ) ( Origene Technologies ) .
Western blot analysis of NAE1 polyclonal antibody ( Cat # PAB2845 ) in mouse brain tissue lysates ( 35 µg/lane ) . NAE1 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
APPBP1 MaxPab polyclonal antibody. Western Blot analysis of APPBP1 expression in rat brain.
Western Blot analysis of NAE1 expression in transfected 293T cell line (H00008883-T01) by NAE1 MaxPab polyclonal antibody.Lane 1: APPBP1 transfected lysate(58.74 KDa).Lane 2: Non-transfected lysate.
Formalin-fixed and paraffin-embedded human testis tissue reacted with APPBP1 antibody (C-term) (AP13996PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of APPBP1 (arrow) using rabbit polyclonal APPBP1 Antibody (C-term) (AP13996PU-N ). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the APPBP1 gene (Lane 2) (Origene Technologies).
Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue reacted with APPBP1 Antibody (Center) Cat.-No AP52806PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining.
Western blot analysis of APPBP1 Antibody (Center) Cat.-No AP52806PU-N in K562,293 cell line lysates (35ug/lane).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NAE1 (RC201326, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NAE1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NAE1 (RC201326, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NAE1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NAE1 (RC201326, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NAE1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NAE1 (RC201326, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NAE1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NAE1 (RC201326, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NAE1.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804384)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804384)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804384)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NAE1 (RC201326, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NAE1.
Western blot analysis of anti-APPBP1 Antibody (C-term) (Cat.#TA300061) in mouse brain tissue lysates (35ug/lane). APPBP1(arrow) was detected using the purified Pab.
Western blot analysis of APP-BP1(arrow) using rabbit polyclonal APP-BP1 Antibody (C-term) (Cat.#TA300061). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the APP-BP1 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with anti-APPBP1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Host: Rabbit; Target Name: Nae1; Sample Tissue: Mouse Lung lysates; Antibody Dilution: 1.0 ug/ml
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-3: Human fetal muscle tissue, Human kidney tissue, Human liver tissue, Primary antibody: (NAE1 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using (NAE1 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using (NAE1 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA804384 | APPBP1 antibody |
||||||
| Purified NAE1 mouse monoclonal antibody, clone OTI1E10 (formerly 1E10) | |||||||
 |
Mouse | IgG1 | OTI1E10 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody!
|
||||||
| TA300061 | APPBP1 (C-term) antibody |
||||||
| APPBP1 rabbit polyclonal  antibody | |||||||
 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA349491 | APPBP1 antibody |
||||||
| Rabbit Polyclonal Anti-NAE1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA349492 | APPBP1 antibody |
||||||
| Rabbit Polyclonal Anti-NAE1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA804228 | APPBP1 antibody |
||||||
| NAE1 mouse monoclonal antibody, clone OTI1A10 (formerly 1A10) | |||||||
 |
Mouse | IgG2b | OTI1A10 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| TA804283 | APPBP1 antibody |
||||||
| NAE1 mouse monoclonal antibody, clone OTI1B2 (formerly 1B2) | |||||||
 |
Mouse | IgG2a | OTI1B2 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| TA804284 | APPBP1 antibody |
||||||
| Purified NAE1 mouse monoclonal antibody, clone OTI1A8 (formerly 1A8) | |||||||
 |
Mouse | IgG1 | OTI1A8 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| TA804320 | APPBP1 antibody |
||||||
| NAE1 mouse monoclonal antibody, clone OTI1D12 (formerly 1D12) | |||||||
 |
Mouse | IgG2b | OTI1D12 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| TA804321 | APPBP1 antibody |
||||||
| Purified NAE1 mouse monoclonal antibody, clone OTI1E9 (formerly 1E9) | |||||||
 |
Mouse | IgG1 | OTI1E9 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| AP13996PU-N | APPBP1 (C-term) antibody |
||||||
 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AP52806PU-N | APPBP1 (Center) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN201326 | NAE1 - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN201326BN | NAE1 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN201326LP | NAE1 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN201326RB | NAE1 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301326 | APPBP1 (transcript variant 3) |
||||
| Recombinant protein of human NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 3 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY418361 | NAE1 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY422668 | NAE1 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR205725L2V | Lenti ORF particles, Nae1 (GFP-tagged ORF) - Rat NEDD8 activating enzyme E1 subunit 1 (Nae1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,230.00
|
|
| OriGene Technologies, Inc. | ||
| RR205725L1V | Lenti ORF particles, Nae1 (Myc-DDK-tagged ORF) - Rat NEDD8 activating enzyme E1 subunit 1 (Nae1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||