ARF5 antibody
Principal name
ARF5 antibody
Alternative names for ARF5 antibody
ADP-ribosylation factor 5
Ncbi ID
9606, NP_001653, NP_001653.1, NP_031506.1, NM_007480, NM_024149
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB)
Background of ARF5 antibody
- Kit Component:
- - KN301473G1, Arf5 gRNA vector 1 in pCas-Guide vector
- KN301473G2, Arf5 gRNA vector 2 in pCas-Guide vector
- KN301473D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Fucini R V., et al. (2000) J Biol Chem. 275:18824-18829.
Immunofluorescence of monoclonal antibody to ARF5 on HeLa cell. [antibody concentration 15 ug/ml]
Western Blot analysis of ARF5 expression in transfected 293T cell line by ARF5 monoclonal antibody (M01), clone 1B4.Lane 1: ARF5 transfected lysate(20.5 KDa).Lane 2: Non-transfected lysate.
ARF5 monoclonal antibody (M01), clone 1B4. Western Blot analysis of ARF5 expression in HeLa ( Cat # L013V1 ).
ARF5 monoclonal antibody (M01), clone 1B4 Western Blot analysis of ARF5 expression in A-431 ( Cat # L015V1 ).
ARF5 monoclonal antibody (M01), clone 1B4. Western Blot analysis of ARF5 expression in Raw 264.7(Cat # L024V1 ).
ARF5 monoclonal antibody (M01), clone 1B4. Western Blot analysis of ARF5 expression in PC-12(Cat # L012V1 ).
Detection limit for recombinant GST tagged ARF5 is approximately 0.3ng/ml as a capture antibody.
Western Blot analysis of ARF5 expression in transfected 293T cell line by ARF5 monoclonal antibody (M02), clone 1B6.Lane 1: ARF5 transfected lysate(20.5 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged ARF5 is approximately 0.3ng/ml as a capture antibody.
Recombinant human ARF5, 1-180aa, His-tagged
Sample(30 ug of whole cell lysate). A:MOLT4. B:Raji. 15% SDS PAGE. TA308198 diluted at 1:1500
ARF5 antibody detects ARF5 protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. ARF5 antibody (TA308198) dilution: 1:500.
Immunofluorescence analysis of methanol-fixed HeLa, using ARF5(TA308198) antibody at 1:500 dilution.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308198 | ARF5 antibody |
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| Rabbit polyclonal antibody to ARF5 (ADP-ribosylation factor 5) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | ICC/IF, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301363 | ARF5 |
||||
| Recombinant protein of human ADP-ribosylation factor 5 (ARF5) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR213765L2V | Lenti ORF particles, Arf5 (GFP-tagged) - Mouse ADP-ribosylation factor 5 (Arf5), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR213765L1V | Lenti ORF particles, Arf5 (Myc-DDK-tagged) - Mouse ADP-ribosylation factor 5 (Arf5), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR215097L2V | Lenti ORF particles, Arf5 (GFP-tagged ORF) - Rat ADP-ribosylation factor 5 (Arf5), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR215097L1V | Lenti ORF particles, Arf5 (Myc-DDK-tagged ORF) - Rat ADP-ribosylation factor 5 (Arf5), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||