Arfaptin-1 antibody
Principal name
Arfaptin-1 antibody
Alternative names for Arfaptin-1 antibody
ARFIP1, ADP-ribosylation factor-interacting protein 1
Ncbi ID
9606, NP_001020764.1, NP_001020764, NP_055262, NP_001020766, NM_021763, NM_001025595
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of Arfaptin-1 antibody
- Kit Component:
- - KN210747G1, ARFIP1 gRNA vector 1 in pCas-Guide vector
- KN210747G2, ARFIP1 gRNA vector 2 in pCas-Guide vector
- KN210747D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Kanoh H., et al. (1997) J. Biol. Chem. 272:5421-5429Williger B.-T., et al. (1999) FEBS Lett. 454:85-89
Immunoperoxidase of monoclonal antibody to ARFIP1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]
Western Blot analysis of ARFIP1 expression in transfected 293T cell line by ARFIP1 monoclonal antibody (M05), clone 1F10.Lane 1: ARFIP1 transfected lysate(41.7 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of ARFIP1 expression in transfected 293T cell line (H00027236-T01) by ARFIP1 MaxPab polyclonal antibody.Lane 1: ARFIP1 transfected lysate(41.03 KDa).Lane 2: Non-transfected lysate.
ARFIP1 MaxPab polyclonal antibody. Western Blot analysis of ARFIP1 expression in HepG2 .
Immunohistochemistry analysis of Arfaptin-1 antibody Cat.-No AP01383PU-N in paraffin-embedded human thymus gland tissue.
Western blot analysis of Arfaptin-1 antibody Cat.-No AP01383PU-N at 1/500 in:
Lane 1: HeLa whole cell lysate.
Lane 2: SP2/0 whole cell lysate.
Lane 3: PC12 whole cell lysate.
Lane 4: Rat muscle tissue lysate.
Lane 1: HeLa whole cell lysate.
Lane 2: SP2/0 whole cell lysate.
Lane 3: PC12 whole cell lysate.
Lane 4: Rat muscle tissue lysate.
Human 293T; WB Suggested Anti-ARFIP1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 293T cell lysate. ARFIP1 is supported by BioGPS gene expression data to be expressed in HEK293T.; ARFIP1 antibody - C-terminal region (ARP54460_P050) in Human 293T cells using Western Blot
ARFIP1 Antibody (Center) (Cat. #AP12992c)immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ARFIP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
ARFIP1 Antibody (Center) (Cat. #AP50226PU-N) western blot analysis in MCF-7 cell line lysates (35µg/lane).This demonstrates the ARFIP1 antibody detected the ARFIP1 protein (arrow).
Immunohistochemical analysis of ARFIP1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of ARFIP1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of ARFIP1 expression in HeLa (A); SP2/0 (B); PC12 (C); rat muscle (D) whole cell lysates.
Western blot analysis of extracts from Jurkat cells, using ARFIP1 antibody.The lane on the right is treated with the synthesized peptide.
Immunofluorescence analysis of A549 cells, using ARFIP1 antibody.The picture on the right is treated with the synthesized peptide.
WB Suggested Anti-ARFIP1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: 293T cell lysateARFIP1 is supported by BioGPS gene expression data to be expressed in HEK293T
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA315373 | Arfaptin-1 antibody |
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| Rabbit polyclonal anti-ARFIP1 antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, ICC/IF, WB |
0.1 ml /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| AP01383PU-N | Arfaptin-1 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP50226PU-N | Arfaptin-1 (Center) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760346 | Arfaptin-1 (full length, N-term HIS tag, transcript variant 2) |
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| Purified recombinant protein of Homo sapiens ADP-ribosylation factor interacting protein 1 (ARFIP1), transcript variant 2, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
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Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR213500L2V | Lenti ORF particles, Arfip1 (GFP-tagged ORF) - Rat ADP-ribosylation factor interacting protein 1 (Arfip1), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR213500L1V | Lenti ORF particles, Arfip1 (Myc-DDK-tagged ORF) - Rat ADP-ribosylation factor interacting protein 1 (Arfip1), 200 uL, >10^7 TU/mL |
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|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||