ARHGAP22 antibody
Principal name
ARHGAP22 antibody
Alternative names for ARHGAP22 antibody
Rho GTPase-activating protein 22, Rho-type GTPase-activating protein 22, p68RacGAP, RHOGAP2
Ncbi ID
NP_067049.2, NP_067049, ADR83111.1, NM_021226, NP_722495.3, BC038272, NM_153800
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of ARHGAP22 antibody
- Kit Component:
- - KN301496G1, Arhgap22 gRNA vector 1 in pCas-Guide vector
- KN301496G2, Arhgap22 gRNA vector 2 in pCas-Guide vector
- KN301496D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
ARHGAP22 Antibody (C-term) (Cat. #AP50235PU-N) western blot analysis in MDA-MB231,WiDr,ZR-75-1 cell line lysates (35µg/lane).This demonstrates the ARHGAP22 antibody detected the ARHGAP22 protein (arrow).
Immunohistochemical analysis of ARHGAP22 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of ARHGAP22 expression in HeLa (A); mouse kidney (B); rat kidney (C) whole cell lysates.
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-ARHGAP22 Rabbit Polyclonal antibody. (TA590468)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ARHGAP22 Rabbit Polyclonal antibody. (TA590468)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-ARHGAP22 Rabbit Polyclonal antibody. (TA590468)
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-ARHGAP22 Rabbit Polyclonal antibody. (TA590468)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ARHGAP22 Rabbit Polyclonal antibody. (TA590468)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ARHGAP22 Rabbit Polyclonal antibody. (TA590468)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-ARHGAP22 Rabbit Polyclonal antibody. (TA590468)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-ARHGAP22 Rabbit Polyclonal antibody. (TA590468)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ARHGAP22 Rabbit Polyclonal antibody. (TA590468)
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ARHGAP22 Rabbit Polyclonal antibody. (TA590468)
Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & plasma membrane.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARHGAP22 (RC220614, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARHGAP22.
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
ELISA: ARHGAP22 Antibody
WB Suggested Anti-ARHGAP22 Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Kidney
Host:Rabbit; Target Name:ARHGAP22; Sample Tissue:Human 721_B; Antibody Dilution: 1.0ug/mlARHGAP22 is supported by BioGPS gene expression data to be expressed in 721_B
Host:Rabbit; Target Name:ARHGAP22; Sample Tissue:Human Fetal Lung; Antibody Dilution: 1.0ug/ml
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP50235PU-N | ARHGAP22 (C-term) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP320614 | ARHGAP22 |
||||
| Purified recombinant protein of Homo sapiens Rho GTPase activating protein 22 (ARHGAP22) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP760689 | ARHGAP22 (full length, N-term HIS tag) |
||||
| Purified recombinant protein of Human Rho GTPase activating protein 22 (ARHGAP22), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY412001 | ARHGAP22 overexpression lysate |
|
 |
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR218963L2V | Lenti ORF particles, Arhgap22 (GFP-tagged) - Mouse Rho GTPase activating protein 22 (Arhgap22), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,460.00
|
|
| OriGene Technologies, Inc. | ||
| MR208068L2V | Lenti ORF particles, Arhgap22 (GFP-tagged) - Mouse Rho GTPase activating protein 22 (cDNA clone MGC:47316 IMAGE:4237331), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,240.00
|
|
| OriGene Technologies, Inc. | ||
| MR218963L1V | Lenti ORF particles, Arhgap22 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 22 (Arhgap22), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,320.00
|
|
| OriGene Technologies, Inc. | ||
| MR208068L1V | Lenti ORF particles, Arhgap22 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 22 (cDNA clone MGC:47316 IMAGE:4237331), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||