ARHGEF1 antibody
Principal name
ARHGEF1 antibody
Alternative names for ARHGEF1 antibody
p115-RhoGEF, p115RhoGEF, Sub1.5, Rho guanine nucleotide exchange factor 1, 115 kDa guanine nucleotide exchange factor
Ncbi ID
9606, NP_004697, NP_945328.1, NP_004697.2, NM_198977, NM_199002, NP_945353, NP_001123622.1, NM_001130152, NM_001130150, NM_001130151, NM_001130153, NM_008488, NM_004706
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Paraffin Sections (P)
Background of ARHGEF1 antibody
- Kit Component:
- - KN301522G1, Arhgef1 gRNA vector 1 in pCas-Guide vector
- KN301522G2, Arhgef1 gRNA vector 2 in pCas-Guide vector
- KN301522D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Detection limit for recombinant GST tagged ARHGEF1 is approximately 3ng/ml as a capture antibody.
Detection limit for recombinant GST tagged ARHGEF1 is approximately 1ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to ARHGEF1 on HeLa cell. [antibody concentration 10 ug/ml]
ARHGEF1 monoclonal antibody (M03), clone 4C4 Western Blot analysis of ARHGEF1 expression in K-562 ( Cat # L009V1 ).
Detection limit for recombinant GST tagged ARHGEF1 is approximately 0.3ng/ml as a capture antibody.
ARHGEF1 polyclonal antibody (A01), Lot # 060726QCS1 Western Blot analysis of ARHGEF1 expression in K-562 ( Cat # L009V1 ).
Western Blot analysis of ARHGEF1 expression in transfected 293T cell line (H00009138-T01) by ARHGEF1 MaxPab polyclonal antibody.Lane 1: ARHGEF1 transfected lysate(102.40 KDa).Lane 2: Non-transfected lysate.
ARHGEF1 MaxPab rabbit polyclonal antibody. Western Blot analysis of ARHGEF1 expression in A-431.
ARHGEF1 Antibody (N-term) (Cat. #AP50237PU-N) western blot analysis in CEM cell line lysates (35µg/lane).This demonstrates the ARHGEF1 antibody detected the ARHGEF1 protein (arrow).
Western blot analysis of ARHGEF1 expression in HuvEc (A); Raw264.7 (B) whole cell lysates.
Western blot analysis of extracts from COLO205, HepG2, HuvEc and Jurkat cells, using ARHGEF1 antibody.The lane on the right is treated with the synthesized peptide.
WB Suggested Anti-ARHGEF1 Antibody; Titration: 1.0 ug/ml; Positive Control: Jurkat Whole Cell ARHGEF1 is supported by BioGPS gene expression data to be expressed in Jurkat
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (ARHGEF1 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using (ARHGEF1 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA314863 | ARHGEF1 antibody |
||||||
| Rabbit polyclonal anti-ARHGEF1 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA349509 | ARHGEF1 antibody |
||||||
| Rabbit Polyclonal Anti-ARHGEF1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
| AP50237PU-N | ARHGEF1 (N-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP321180 | ARHGEF1 (transcript variant 1) |
||||
| Recombinant protein of human Rho guanine nucleotide exchange factor (GEF) 1 (ARHGEF1), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€788.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY404700 | ARHGEF1 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY404714 | ARHGEF1 overexpression lysate |
|
|
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR211176L2V | Lenti ORF particles, Arhgef1 (GFP-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 1 (Arhgef1), transcript variant 3, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,780.00
|
|
| OriGene Technologies, Inc. | ||
| MR211176L1V | Lenti ORF particles, Arhgef1 (Myc-DDK-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 1 (Arhgef1), transcript variant 3, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,600.00
|
|
| OriGene Technologies, Inc. | ||