Switch to Europe/Worldwide website Switch to German website Switch to French website Switch to OriGene Technologies, Inc. (North America)
Home » Target » ARHGEF2 antibody

ARHGEF2 antibody

(reset)

Principal name

ARHGEF2 antibody

Alternative names for ARHGEF2 antibody

KIAA0651, LFP40, GEF-H1, Rho guanine nucleotide exchange factor 2, Guanine nucleotide exchange factor H1, Microtubule-regulated Rho-GEF, Proliferating cell nucleolar antigen p40

SwissProt ID

B2DCZ9 (Pig), Q5FVC2 (Rat), Q60875 (Mouse), Q865S3 (Canfa), Q92974 (Human)

Gene ID

9181 (ARHGEF2), 16800 (Arhgef2), 310635

Ncbi ID

9606, NP_001185840.1, NP_004714, NM_001162383, NP_001155855.1, ABM85604.1, AAH20567, NM_004723, NM_008487, NM_001012079, NM_001162384

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Por (Porcine), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Sheep, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C), Paraffin Sections (P)

Background of ARHGEF2 antibody

GEFH1 is an ~110 kDa protein belonging to the Dbl family of proto-oncogenes. GEF-H1 can activate the small GTPase RhoA, but not Rac1 or Cdc42. Rho family GTPases are central regulators of epithelial tight junctions and the cytoskeleton. GEF-H1 can associate with different cytoskeletal structures, namely microtubules and the actin cytoskeleton. It has also been proposed to mediate crosstalk between the two types of filaments.
Localization of GEF-H1 differs between cell types. In MRC-5 fibroblast cells, GEF-H1 localizes to stress fibers. In epithelial cells, GEF-H1 is associated with apical tight junctions and involved in regulating paracellular permeability of small hydrophilic tracers. Furthermore, its subcellular localization changes in mitotic cells, where endogenous GEF-H1 is concentrated at mitotic spindles. GEF-H1 is capable of binding to the F-actin binding junctional adaptor cingulin. Binding to cingulin inhibits GEF-H1 and results in the downregulation of RhoA and inhibition of G1/S phase transition. In low confluent cultured cells, the localization of GEF-H1 is predominantly cytoplasmic. With increasing density of the cells, free GEF-H1 is sequestered at the tight junctions by cingulin.
GEF-H1 is part of the signaling pathway connecting epithelial polarity with the cell cycle, and as such involved in oncogenesis.

AP05081PU-N - ARHGEF2
Western blot analysis using anti-Lfc antibody on HEK-293 cell transfected with vector alone (Lane 1), LFC-short (Lane 2) and LFC-long (Lane 3).
PAB10113 - ARHGEF2
Western blot analysis using Arhgef2 polyclonal antibody ( Cat # PAB10113 ) on HEK293 cell transfected with vector alone (lane 1), Arhgef2-short (lane 2) and Arhgef2-long (lane 3).
AP20505PU-N - ARHGEF2
Western blot (WB) analysis of ARHGEF2 antibody (Cat.-No.: AP20505PU-N) in extracts from NIH cells.
A1233-1 - ARHGEF2
Western blot analysis of extracts from HeLa cells treated with TSA 400nM 24H and Jurkat cells treated with forskolin 40nM 30', using Rho/Rac Guanine Nucleotide Exchange Factor 2 (Phospho-Ser885) Antibody. The lane on the right is treated with the synthesized peptide.
B1233-1 - ARHGEF2
Western blot analysis of extracts from NIH-3T3 cells, using Rho/Rac Guanine Nucleotide Exchange Factor 2 (Ab-885) Antibody. The lane on the right is treated with the synthesized peptide.
C18399-1 - ARHGEF2
Western blot analysis of extracts from RAW264.7 cells, using ARHGEF2 Antibody. The lane on the right is treated with the synthesized peptide.
ARP59669_P050 - ARHGEF2
Human Placenta; WB Suggested Anti-ARHGEF2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Placenta; ARHGEF2 antibody - C-terminal region (ARP59669_P050) in Human Placenta cells using Western Blot
CPA2367-100ul - ARHGEF2
Western blot analysis of GEF H1 expression in HeLa (A); NIH3T3 (B); H9C2 (C) whole cell lysates.
TP304546 - ARHGEF2
CPA4568-100ul - ARHGEF2
Western blot analysis of GEF H1 expression in Hela (A); Jurkat (B); NIH3T3 (C) whole cell lysates.
TA314873 - ARHGEF2
Western blot analysis of extracts from RAW264.7 cells, using ARHGEF2 antibody.The lane on the right is treated with the synthesized peptide.
TA332548 - ARHGEF2
Western blot analysis of extracts of various cell lines, using ARHGEF2 antibody.
TA332548 - ARHGEF2
Immunohistochemical of paraffin-embedded rat lung using ARHGEF2 antibody at dilution of 1:200 (400x lens).
TA342688 - ARHGEF2
WB Suggested Anti-ARHGEF2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human Placenta
TA349506 - ARHGEF2
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using (ARHGEF2 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
TA349506 - ARHGEF2
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using (ARHGEF2 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
TA314874 - ARHGEF2
Western blot analysis of extracts from HeLa cells treated with TSA (400nM, 24hours) and Jurkat cells treated with forskolin(40nM, 30mins), using Rho/Rac Guanine Nucleotide Exchange Factor 2 (Phospho-Ser885) antibody.The lane on the right is treated with t
TA314874 - ARHGEF2
Western blot analysis of extracts from 293 cells (Lane 2), using Rho/Rac Guanine Nucleotide Exchange Factor 2 (Phospho-Ser885) Antibody. The lane on the left is treated with synthesized peptide.

13 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ARHGEF2 antibody
  Rabbit polyclonal anti-ARHGEF2 antibody  
Western blot analysis of extracts from RAW264.7 cells, using ARHGEF2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Rt E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

ARHGEF2 antibody
  Rabbit polyclonal Rho/Rac Guanine Nucleotide Exchange Factor 2 (Ser885) antibody(Phospho-specific)  
Western blot analysis of extracts from HeLa cells treated with TSA (400nM, 24hours) and Jurkat cells treated with forskolin(40nM, 30mins), using Rho/Rac Guanine Nucleotide Exchange Factor 2 (Phospho-Ser885) antibody.The lane on the right is treated with t Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

ARHGEF2 antibody
  Rabbit anti-ARHGEF2 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using ARHGEF2 antibody. Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 mg / €335.00
  OriGene Technologies, Inc.
+1 additional image

ARHGEF2 antibody
  Rabbit Polyclonal Anti-ARHGEF2 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using (ARHGEF2 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

ARHGEF2 antibody
ARHGEF2 Mouse IgG1 B4/7 Purified Can, Hu, Ms C, ICC/IF, IP, WB
0.1 mg / €490.00
  OriGene Technologies GmbH

ARHGEF2 antibody
Western blot analysis using anti-Lfc antibody on HEK-293 cell transfected with vector alone (Lane 1), LFC-short (Lane 2) and LFC-long (Lane 3). Sheep IgG Purified Ms ICC/IF, IP, WB
0.25 mg / €400.00
  OriGene Technologies GmbH

ARHGEF2 antibody
Western blot (WB) analysis of ARHGEF2 antibody (Cat.-No.: AP20505PU-N) in extracts from NIH cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €370.00
  OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ARHGEF2
  Recombinant protein of human rho/rac guanine nucleotide exchange factor (GEF) 2 (ARHGEF2)  
ARHGEF2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €788.00
  OriGene Technologies, Inc.
 

Lysates

Catalog No.    

ARHGEF2 overexpression lysate
ARHGEF2 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Arhgef2 (GFP-tagged) - Mouse rho/rac guanine nucleotide exchange factor (GEF) 2 (Arhgef2), transcript variant 1, 200 uL, >10^7 TU/mL
Lenti ORF particles, Arhgef2 (GFP-tagged) - Mouse rho/rac guanine nucleotide exchange factor (GEF) 2 (Arhgef2), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,870.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Arhgef2 (Myc-DDK-tagged) - Mouse rho/rac guanine nucleotide exchange factor (GEF) 2 (Arhgef2), transcript variant 1, 200 uL, >10^7 TU/mL
Lenti ORF particles, Arhgef2 (Myc-DDK-tagged) - Mouse rho/rac guanine nucleotide exchange factor (GEF) 2 (Arhgef2), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,680.00
  OriGene Technologies, Inc.
 

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Arhgef2 (GFP-tagged ORF) - Rat rho/rac guanine nucleotide exchange factor (GEF) 2 (Arhgef2), 200 uL, >10^7 TU/mL
Lenti ORF particles, Arhgef2 (GFP-tagged ORF) - Rat rho/rac guanine nucleotide exchange factor (GEF) 2 (Arhgef2), 200 uL, >10^7 TU/mL  
200 µl / €1,870.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Arhgef2 (Myc-DDK-tagged ORF) - Rat rho/rac guanine nucleotide exchange factor (GEF) 2 (Arhgef2), 200 uL, >10^7 TU/mL
Lenti ORF particles, Arhgef2 (Myc-DDK-tagged ORF) - Rat rho/rac guanine nucleotide exchange factor (GEF) 2 (Arhgef2), 200 uL, >10^7 TU/mL  
200 µl / €1,680.00
  OriGene Technologies, Inc.
 

13 Item(s)

per page
13 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. Lysate (1)
  2. Mouse Lenti ORF Particle (2)
  3. Primary Antibody (7)
  4. Protein | Growth Factor (1)
  5. Rat Lenti ORF Particle (2)
Clonality
  1. Polyclonal (6)
  2. Monoclonal (1)
Target
  1. ARHGEF2 (8)
Reactivity
  1. Human (Hu) (6)
  2. Mouse (Ms) (6)
  3. Rat (Rt) (4)
  4. Canine (Can) (1)
Applications
  1. Western blot / Immunoblot (WB) (6)
  2. Enzyme Immunoassay (E) (3)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (2)
  4. Immunoprecipitation (IP) (2)
  5. Paraffin Sections (P) (2)
  6. Frozen sections (C) (1)
Presentation
  1. Purified (6)
  2. Aff - Purified (1)
Host
  1. Rabbit (5)
  2. Mouse (1)
  3. Sheep (1)
Images
  1. 1 image (7)
Images: Applications
  1. Western blot / Immunoblot (WB) (5)
  2. IHC (2)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (13)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
© Copyright 1998 - 2018 OriGene Technologies GmbH
  • LinkedIn