ARL3 antibody
Principal name
ARL3 antibody
Alternative names for ARL3 antibody
ARFL3, ADP-ribosylation factor-like protein 3
Ncbi ID
9606, NP_004302.1, NP_004302, AAH09841, NP_062692.1, NM_022700, NM_004311
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of ARL3 antibody
ADP-ribosylation factors (ARFs) are low molecular weight GTP-binding proteins belonging to the RAS superfamily. The predicted 182-amino acid ARL3 (ADP-ribosylation like factor) protein shares 97% amino acid identity with rat ARLl3 and 43% identity with human ARF1. Like the ARFs, ARL3 has a glycine at position 2, the site of N myristoylation, and lacks cysteine residues near the C terminus, which are found in other members of the RAS family. Northern blot analysis detected a 1-kb ARL3 transcript in all tissues tested, with highest expression in heart and lung, and lower expression in brain, liver, kidney, ovary, and testis. A 5.5-kb transcript was also detected in most tissues, with highest expression in brain. Immunoblot analysis detected ARL3 in human tumor cell lines but not in normal rodent cells. Although ARL3 binds GTP, it is devoid of activity in the cholera toxin-dependent ADP-ribosylation of Gs, and is therefore classified as an ARF-like protein.
General readings
Bartolini F. et al. (2002) J Biol Chem. 277:14629-14634.Hillig R.C. et al. (2001) Structure. 8:1239-1245.
Immunofluorescence of purified MaxPab antibody to ARL3 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of ARL3 expression in transfected 293T cell line (H00000403-T02) by ARL3 MaxPab polyclonal antibody.Lane 1: ARL3 transfected lysate(20.02 KDa).Lane 2: Non-transfected lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with ARL3 polyclonal antibody ( Cat # PAB4262 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
Western blot analysis of ARL3 ( arrow ) using rabbit ARL3 polyclonal antibody ( Cat # PAB4262 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the ARL3 gene ( Lane 2 ) ( Origene Technologies ) .
Western blot analysis of ARL3 polyclonal antibody ( Cat # PAB4262 ) in A2058 cell line lysate ( 35 µg/lane ) . ARL3 ( arrow ) was detected using the purified ARL3 polyclonal antibody ( Cat # PAB4262 ) .
Immunofluorescence of purified MaxPab antibody to ARL3 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of ARL3 expression in transfected 293T cell line (H00000403-T02) by ARL3 MaxPab polyclonal antibody.Lane 1: ARL3 transfected lysate(20.02 KDa).Lane 2: Non-transfected lysate.
Western blot analysis using anti-ARL3 (N-term) Pab (Cat.#AP12134PU-N).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
(LEFT) Western blot analysis of anti-ARL3 (Cat. #AP12135PU-N) in A2058 cell line lysate (35 µg/lane).ARL3 (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of ARL3 (arrow) using rabbit polyclonal ARL3 Antibody (C-term) (Cat.#AP12135PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ARL3 gene (Lane 2) (Origene Technologies).
Human MCF-7; WB Suggested Anti-ARL3 Antibody. . Titration: 1.0 ug/ml. . Positive Control: MCF7 Whole Cell. ARL3 is supported by BioGPS gene expression data to be expressed in MCF7.; ARL3 antibody - N-terminal region (ARP59667_P050) in Human MCF-7 cells using Western Blot
Human 721_B; Host:Rabbit. Target Name:ARL3. Sample Tissue:Human 721_B. Antibody Dilution: 1.0ug/ml. ARL3 is supported by BioGPS gene expression data to be expressed in 721_B.; ARL3 antibody - N-terminal region (ARP59667_P050) in Human 721_B cells using Western Blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARL3 (RC203469, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARL3.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ARL3 mouse monoclonal antibody. (TA803086) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ARL3 mouse monoclonal antibody. (TA803086) Dilution: 1:150
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARL3 (RC203469, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARL3.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ARL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA803087)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ARL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA803087)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ARL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA803087)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ARL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA803087)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARL3 (RC203469, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARL3.
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-ARL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA803088)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARL3 (RC203469, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARL3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARL3 (RC203469, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARL3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARL3 (RC203469, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARL3.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ARL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA803091)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ARL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA803091)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ARL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA803091)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARL3 (RC203469, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARL3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARL3 (RC203469, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARL3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARL3 (RC203469, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARL3.
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA803087 | ARL3 antibody |
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| ARL3 mouse monoclonal antibody, clone OTI1C6 (formerly 1C6) | |||||||
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Mouse | IgG2a | OTI1C6 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody!
|
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| TA803091 | ARL3 antibody |
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| ARL3 mouse monoclonal antibody, clone OTI2A9 (formerly 2A9) | |||||||
 |
Mouse | IgG2a | OTI2A9 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA803085 | ARL3 antibody |
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| ARL3 mouse monoclonal antibody, clone OTI7E5 (formerly 7E5) | |||||||
 |
Mouse | IgG2b | OTI7E5 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA803086 | ARL3 antibody |
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| ARL3 mouse monoclonal antibody, clone OTI3A10 (formerly 3A10) | |||||||
 |
Mouse | IgG2a | OTI3A10 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA803088 | ARL3 antibody |
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| ARL3 mouse monoclonal antibody, clone OTI2G7 | |||||||
 |
Mouse | IgG2a | OTI2G7 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA803089 | ARL3 antibody |
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| ARL3 mouse monoclonal antibody, clone OTI3E1 (formerly 3E1) | |||||||
 |
Mouse | IgG1 | OTI3E1 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA803090 | ARL3 antibody |
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| ARL3 mouse monoclonal antibody, clone OTI5E6 (formerly 5E6) | |||||||
 |
Mouse | IgG2b | OTI5E6 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA803092 | ARL3 antibody |
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| ARL3 mouse monoclonal antibody, clone OTI3C6 (formerly 3C6) | |||||||
 |
Mouse | IgG2a | OTI3C6 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA803158 | ARL3 antibody |
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| ARL3 mouse monoclonal antibody, clone OTI3D3 (formerly 3D3) | |||||||
 |
Mouse | IgG2a | OTI3D3 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| AP12134PU-N | ARL3 (N-term) antibody |
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 |
Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| AP12135PU-N | ARL3 (C-term) antibody |
||||||
 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP303469 | ARL3 |
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| Recombinant protein of human ADP-ribosylation factor-like 3 (ARL3) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR211638L2V | Lenti ORF particles, Arl3 (GFP-tagged ORF) - Rat ADP-ribosylation factor-like 3 (Arl3), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR211638L1V | Lenti ORF particles, Arl3 (Myc-DDK-tagged ORF) - Rat ADP-ribosylation factor-like 3 (Arl3), 200 uL, >10^7 TU/mL |
|
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200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||