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Artemin antibody

Principal name

Artemin antibody

Alternative names for Artemin antibody

Enovin, Neublastin, ARTN, EVN

SwissProt ID

Q5T4W7 (Human), Q6AYE8 (Rat), Q9Z0L2 (Mouse)

Gene ID

11876 (Artn), 9048 (ARTN), 362572

Ncbi ID

NP_033841.1, NP_001129687.1, 9606, NP_001129687, NM_057090, NM_057091, NM_001136215, NP_476431, NM_053397

Available reactivities

Ms (Mouse), Hu (Human), Mky (Monkey), Prim (Primate), Rt (Rat)

Available hosts

Goat, Rabbit, Mouse, Rat

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Artemin antibody

Artemin is a member of the Glia Cel-lDerived Neurotrophic factor (GDNF) family ligands, which include GDNF, Persephin, Artemin, and Neurturin. GDNF family ligands are distant members of the Transforming Growth Factor β (TGFβ) superfamily. Similar to other TGFβ family proteins, Artemin is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxyterminal domain. The carboxy terminal domain of Artemin contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine-knot and the single interchain disulfide bond. Biologically active Artemin is a disulfide-linked homodimer of the carboxyterminal 113 amino acid residues. Mature mouse Artemin shares 88.5% amino acid sequence similarity with human Artemin. Mature Artemin also shares approximately 40% amino acid sequence identity with the other three members of the GDNF family ligands. Bioactivities of all GDNF family ligands are mediated through a receptor complex composed of a high affinity ligand binding component (GFRα1GFRα4) and a common signaling component, cRET (receptor tyrosine kinase). Artemin prefers to bind to GFRα3 and activites the GFRα3RET. However, in the presence of RET, it can bind to GFRα1 as well. Artemin has been shown to promote the survival and growth of various peripheral and central neurons, including sympathetic and dopaminergic neurons. It may also play an important role in the development of sympathetic neurons and several organs.

Western Blot analysis of ARTN expression in transfected 293T cell line (H00009048-T01) by ARTN MaxPab polyclonal antibody.Lane 1: ARTN transfected lysate(25.08 KDa).Lane 2: Non-transfected lysate.

H00009048-B01

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

AP23948PU-N

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)

AP23948PU-N

Immunofluorescence analysis of GFRA3-Ret51 receptor complex bound Artemin. Human U2OS cells were transfected with GFRa3-Ret51 expression vector followed by treatment with 100 nM Artemin. Artemin bound to the GFRa3-Ret51 receptor complex was detected by anti-Artemin antibody clone 2A5. Goat anti-mouse Dylight 488 conjugated antibody (dilution 1:1000) was used as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for Artemin and blue for nuclei. A. Artemin bound to the GFRa3-Ret51 receptor complex; B. Negative control - GFRa3-Ret51 receptor expressing U2OS cells

AM33447PU-N

Western Blot testing of purified recombinant Artemin by anti-Artemin antibody 2A5. For analysis HRP-conjugated 2A5 antibody was used. 200 ng of purified recombinant Artemin expressed by CHOEBNALT85 cell line was loaded per lane. Lane 1: Reduced conditions; Line 2: Non-reduced conditions; Lane 3: Protein marker (8003, Naxo)

AM33447PU-N

Immunofluorescence analysis of GFRA3-Ret51 receptor complex bound Artemin. Human U2OS cells were transfected with GFRa3-Ret51 expression vector followed by treatment with 100 nM Artemin. Artemin bound to the GFRa3-Ret51 receptor complex was detected by anti-Artemin antibody clone 2D12. Goat anti-mouse Dylight 488 conjugated antibody (dilution 1:1000) was used as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for Artemin and blue for nuclei. A. Artemin bound to the GFRa3-Ret51 receptor complex; B. Negative control - GFRa3-Ret51 receptor expressing U2OS cells.

AM33448PU-N

Western Blot testing of purified recombinant Artemin by anti-Artemin antibody 2D12. Goat anti-mouse IgG HRP conjugate was used as secondary antibody. 15 µl of CHOEBNALT85 Artemin expressing supernatant was loaded per line. Line 1. Reduced conditions; Line 2. Non-reduced conditions; Line 3. Protein marker (8003, Naxo).

AM33448PU-N

Immunofluorescence analysis of GFRA3-Ret51 receptor complex bound Artemin. Human U2OS cells were transfected with GFRa3-Ret51 expression vector followed by treatment with 100 nM Artemin. Artemin bound to the GFRa3-Ret51 receptor complex was detected by anti-Artemin antibody clone 1F3. Goat anti-mouse Dylight 488 conjugated antibody (dilution 1:1000) was used as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for Artemin and blue for nuclei. A. Artemin bound to the GFRa3-Ret51 receptor complex; B. Negative control - GFRa3-Ret51 receptor expressing U2OS cells.

AM33449PU-N

Immunofluorescence analysis of GFRA3-Ret51 receptor complex bound Artemin. Human U2OS cells were transfected with GFRa3-Ret51 expression vector followed by treatment with 100 nM Artemin. Artemin bound to the GFRa3-Ret51 receptor complex was detected by anti-Artemin antibody clone 2G7. Goat anti-mouse Dylight 488 conjugated antibody (dilution 1:1000) was used as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for Artemin and blue for nuclei. A. Artemin bound to the GFRa3-Ret51 receptor complex; B. Negative control - GFRa3-Ret51 receptor expressing U2OS cells

AM33450PU-N

TP723024

Western Blot: Artemin Antibody - Western Blot analysis of Artemin in human dorsal root ganglia, using anti-Artemin.

TA309576

(0.1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TA311280

Gel: 12%SDS-PAGE, Lysate: 50 ug, Lane 1-3: 293T cells, SP20 cells, human fetal liver tissue, Primary antibody: (ARTN Antibody) at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes

TA350947

Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: A431 cells, 231 cells, Primary antibody: (ARTN Antibody) at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes

TA350631

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA309576	Artemin antibody
	Rabbit Polyclonal Artemin Antibody
	Rabbit			Purified	Hu	WB	0.1 mg / €335.00
							OriGene Technologies, Inc.
TA311280	Artemin antibody
	Goat Anti-ARTN Antibody
	Goat			Aff - Purified	Hu, Ms, Rt	E, WB	0.1 mg / €360.00
							OriGene Technologies, Inc.
TA350631	Artemin antibody
	Rabbit Polyclonal Anti-ARTN Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	E, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA350947	Artemin antibody
	Rabbit Polyclonal Anti-ARTN Antibody
	Rabbit	IgG		Purified	Hu	E, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
AP01145BT-N	Artemin antibody
	Rabbit			Biotin	Hu	E, WB	50 µg / €340.00
							OriGene Technologies GmbH
AP01145BT-S	Artemin antibody
	Rabbit			Biotin	Hu	E, WB	25 µg / €250.00
							OriGene Technologies GmbH
AP01145PU-N	Artemin antibody
	Rabbit			Aff - Purified	Hu	E, WB	0.1 mg / €340.00
							OriGene Technologies GmbH
AP01145PU-S	Artemin antibody
	Rabbit			Aff - Purified	Hu	E, WB	50 µg / €250.00
							OriGene Technologies GmbH
AP23948PU-N	Artemin (127-140) antibody
	Rabbit	IgG		Aff - Purified	Hu	P, WB	50 µg / €425.00
							OriGene Technologies GmbH
+1 additional image
DM3532P	Artemin antibody
	Rat	IgG2	6G32	Purified	Ms	C, WB	0.1 mg / €460.00
							OriGene Technologies GmbH
GT15066-100	Artemin antibody
	Goat			Purified	Ms	C, E, WB	0.1 mg / €450.00
							Neuromics Antibodies
MO15021-500	Artemin antibody
	Mouse	IgG	185230	Purified	Ms	C, E, WB	0.5 mg / €410.00
							Neuromics Antibodies
RA19009-100	Artemin antibody
	Rabbit			Purified	Hu	WB	0.1 ml / €330.00
							Neuromics Antibodies

Proteins & Growth Factors

Catalog No.	Species	Pres.	Purity	Source
TP723024	Artemin (transcript variant 4)
	Purified recombinant protein of Human artemin (ARTN), transcript variant 4.
	Human		> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.	E. coli	20 µg / €230.00
					OriGene Technologies, Inc.

PR27091-5	Artemin
	Human	Purified	> 98 %	E. coli	5 µg / €190.00
					Neuromics Antibodies
PR27091-20	Artemin
	Human	Purified	> 98 %	E. coli	20 µg / €290.00
					Neuromics Antibodies

Lysates

Catalog No.
LY409292	ARTN overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.
LY409293	ARTN overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.
LY427855	ARTN overexpression lysate
		0.1 mg / €315.00
		OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.
RR209126L2V	Lenti ORF particles, Artn (GFP-tagged ORF) - Rat artemin (Artn), 200 uL, >10^7 TU/mL
		200 µl / €930.00
		OriGene Technologies, Inc.

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