ASB8 antibody
Principal name
ASB8 antibody
Alternative names for ASB8 antibody
ASB-8, Ankyrin repeat and SOCS box protein 8
Ncbi ID
9606, NP_077000, NP_077000.1, NM_001170710, NM_001170711, NM_024095
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of ASB8 antibody
Ankyrin repeat and SOCS box containing 8, also known as ASB8, may be a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
General readings
"Molecular cloning and characterization of the human ASB-8 gene encoding a novel member of ankyrin repeat and SOCS box containing protein family." Liu Y., Li J., Zhang F., Qin W., Yao G., He X., Xue P., Ge C., Wan D., Gu J. Biochem. Biophys. Res. Commun. 300:972-979(2003)
Western Blot analysis of ASB8 expression in transfected 293T cell line (H00140461-T01) by ASB8 MaxPab polyclonal antibody.Lane 1: ASB8 transfected lysate(31.68 KDa).Lane 2: Non-transfected lysate.
Immunoperoxidase of monoclonal antibody to ASB8 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3 ug/ml]
Western Blot analysis of ASB8 expression in transfected 293T cell line (H00140461-T01) by ASB8 MaxPab polyclonal antibody.Lane 1: ASB8 transfected lysate(31.68 KDa).Lane 2: Non-transfected lysate.
Human Pancreas; WB Suggested Anti-ASB8 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Pancreas; ASB8 antibody - N-terminal region (ARP52408_P050) in Human Pancreas cells using Western Blot
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ASB8 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808401)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ASB8 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808401)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASB8 (RC201716, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ASB8.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASB8 (RC201716, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ASB8.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASB8 (RC201716, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ASB8.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASB8 (RC201716, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ASB8.(1:2000)
WB Suggested Anti-ASB8 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Pancreas
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA808401 | ASB8 antibody |
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| ASB8 mouse monoclonal antibody, clone OTI3A12 (formerly 3A12) | |||||||
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Mouse | IgG2a | OTI3A12 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA808402 | ASB8 antibody |
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| ASB8 mouse monoclonal antibody, clone OTI2D7 (formerly 2D7) | |||||||
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Mouse | IgG2a | OTI2D7 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
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| TA808403 | ASB8 antibody |
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| ASB8 mouse monoclonal antibody, clone OTI7D2 (formerly 7D2) | |||||||
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Mouse | IgG2b | OTI7D2 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
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| TA808405 | ASB8 antibody |
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| ASB8 mouse monoclonal antibody, clone OTI1A6 (formerly 1A6) | |||||||
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Mouse | IgG2a | OTI1A6 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760052 | ASB8 (full length, N-term HIS tag) |
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| Recombinant protein of human ankyrin repeat and SOCS box containing 8 (ASB8), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
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Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR203923L2V | Lenti ORF particles, Asb8 (GFP-tagged) - Mouse ankyrin repeat and SOCS box-containing 8 (Asb8), transcript variant 2, 200 uL, >10^7 TU/mL |
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 |
200 µl /
€1,240.00
|
|
| OriGene Technologies, Inc. | ||
| MR216127L2V | Lenti ORF particles, Asb8 (GFP-tagged) - Mouse ankyrin repeat and SOCS box-containing 8 (Asb8), transcript variant 3, 200 uL, >10^7 TU/mL |
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|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR203923L1V | Lenti ORF particles, Asb8 (Myc-DDK-tagged) - Mouse ankyrin repeat and SOCS box-containing 8 (Asb8), transcript variant 2, 200 uL, >10^7 TU/mL |
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|
200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||
| MR216127L1V | Lenti ORF particles, Asb8 (Myc-DDK-tagged) - Mouse ankyrin repeat and SOCS box-containing 8 (Asb8), transcript variant 3, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||