ASCC2 antibody
Principal name
ASCC2 antibody
Alternative names for ASCC2 antibody
ASC1P100, ASC-1 complex subunit p100, Trip-4 complex subunit p100, Activating signal cointegrator 1 complex subunit 2
Ncbi ID
9606, NP_115580, NP_115580.2, NP_083567.1, NM_029291, NM_032204
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of ASCC2 antibody
- Kit Component:
- - KN301666G1, Ascc2 gRNA vector 1 in pCas-Guide vector
- KN301666G2, Ascc2 gRNA vector 2 in pCas-Guide vector
- KN301666D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
"DNA unwinding by ASCC3 helicase is coupled to ALKBH3-dependent DNA alkylation repair and cancer cell proliferation." Dango S., Mosammaparast N., Sowa M.E., Xiong L.J., Wu F., Park K., Rubin M., Gygi S., Harper J.W., Shi Y. Mol. Cell 44:373-384(2011)
ASCC2 MaxPab polyclonal antibody. Western Blot analysis of ASCC2 expression in human placenta.
Western Blot analysis of ASCC2 expression in transfected 293T cell line (H00084164-T01) by ASCC2 MaxPab polyclonal antibody.Lane 1: ASCC2 transfected lysate(83.27 KDa).Lane 2: Non-transfected lysate.
ASCC2 MaxPab polyclonal antibody. Western Blot analysis of ASCC2 expression in HeLa.
ASCC2 MaxPab polyclonal antibody. Western Blot analysis of ASCC2 expression in human placenta.
Western Blot analysis of ASCC2 expression in transfected 293T cell line (H00084164-T01) by ASCC2 MaxPab polyclonal antibody.Lane 1: ASCC2 transfected lysate(83.27 KDa).Lane 2: Non-transfected lysate.
ASCC2 MaxPab polyclonal antibody. Western Blot analysis of ASCC2 expression in HeLa.
Human HepG2; WB Suggested Anti-ASCC2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: HepG2 cell lysate. ASCC2 is supported by BioGPS gene expression data to be expressed in HepG2.; ASCC2 antibody - middle region (ARP42671_P050) in Human HepG2 cells using Western Blot
ASCC2 monoclonal antibody (M07), clone 3B2. Western Blot analysis of ASCC2 expression in HeLa(Cat # L013V1 ).
Human HepG2; WB Suggested Anti-ASCC2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: HepG2 cell lysate. ASCC2 is supported by BioGPS gene expression data to be expressed in HepG2.; ASCC2 antibody - middle region (AP43253PU-N) in Human HepG2 cells using Western Blot
Anti-ASCC2 mouse monoclonal antibody (TA502806) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ASCC2(RC203391 ).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASCC2 (RC203391, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ASCC2.
Flow cytometric Analysis of Hela cells, using anti-ASCC2 antibody(TA502806),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Anti-ASCC2 mouse monoclonal antibody (TA503246) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ASCC2(RC203391 ).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASCC2 (RC203391, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ASCC2.
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-3: A431 cells, 293T cells, hela cells, Primary antibody: (ASCC2 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds
WB Suggested Anti-ASCC2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: HepG2 cell lysateASCC2 is supported by BioGPS gene expression data to be expressed in HepG2
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA350949 | ASCC2 antibody |
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| Rabbit Polyclonal Anti-ASCC2 Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| TA502806 | ASCC2 antibody |
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| ASCC2 mouse monoclonal antibody, clone OTI1E8 (formerly 1E8) | |||||||
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Mouse | IgG1 | OTI1E8 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
|
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| TA503246 | ASCC2 antibody |
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| ASCC2 mouse monoclonal antibody, clone OTI2D3 (formerly 2D3) | |||||||
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Mouse | IgG2b | OTI2D3 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP303391 | ASCC2 |
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| Recombinant protein of human activating signal cointegrator 1 complex subunit 2 (ASCC2) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR210450L2V | Lenti ORF particles, Ascc2 (GFP-tagged) - Mouse activating signal cointegrator 1 complex subunit 2 (Ascc2), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€1,540.00
|
|
| OriGene Technologies, Inc. | ||
| MR210450L1V | Lenti ORF particles, Ascc2 (Myc-DDK-tagged) - Mouse activating signal cointegrator 1 complex subunit 2 (Ascc2), 200 uL, >10^7 TU/mL |
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|
200 µl /
€1,380.00
|
|
| OriGene Technologies, Inc. | ||
