ASCC3L1 antibody
Principal name
ASCC3L1 antibody
Alternative names for ASCC3L1 antibody
SNRNP200, HELIC2, KIAA0788, BRR2 homolog, U5 small nuclear ribonucleoprotein 200 kDa helicase, U5 snRNP-specific 200 kDa protein, Activating signal cointegrator 1 complex subunit 3-like
SwissProt ID
O75643 (Human)
Gene ID
23020 (SNRNP200)
Ncbi ID
Available reactivities
Available hosts
Available applications
Immunoprecipitation (IP), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Enzyme Immunoassay (E)
Background of ASCC3L1 antibody
small nuclear ribonucleoprotein 200kDa (U5)
Western Blot: ASCC3L1 Antibody [NBP1-79036]- Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), Jurkat (J; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-SNRNP200 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. SNRNP200 was also immunoprecipitated by rabbit anti-SNRNP200 antibody NBP1-79037, which recognizes a downstream epitope.
Western Blot: ASCC3L1 Antibody [NBP1-79037]-Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), Jurkat (J; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-SNRNP200 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. SNRNP200 was also immunoprecipitated by rabbit anti-SNRNP200 antibody NBP1-79036 which recognizes an upstream epitope.
Immunohistochemistry-Paraffin: ASCC3L1 Antibody [NBP1-90086] - Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.
Immunofluorescence: ASCC3L1 Antibody [NBP1-90086] - 2 OS shows positivity in nucleus but not nucleoli.
Western Blot: ASCC3L1 Antibody [NBP1-90086] Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 | Lane 2: RT-4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil
HeLa cells were subjected to SDS PAGE followed by western blot with 23875-1-AP(SNRNP200 antibody) at dilution of 1:1000
HEK-293 cells were subjected to SDS PAGE followed by western blot with 23875-1-AP(SNRNP200 antibody) at dilution of 1:1000
Jurkat cells were subjected to SDS PAGE followed by western blot with 23875-1-AP(SNRNP200 antibody) at dilution of 1:1000
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23875-1-AP(SNRNP200 antibody) at dilution of 1:1000