ASF1A antibody
Principal name
ASF1A antibody
Alternative names for ASF1A antibody
CCG1-interacting factor A, CGI-98, HSPC146, CIA, Histone chaperone ASF1A, Anti-silencing function protein 1 homolog A
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB)
Background of ASF1A antibody
- Kit Component:
- - KN301672G1, Asf1a gRNA vector 1 in pCas-Guide vector
- KN301672G2, Asf1a gRNA vector 2 in pCas-Guide vector
- KN301672D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Sanematsu F, Takami Y, Barman HK, Fukagawa T, Ono T, Shibahara K, Nakayama T.
Asf1 is required for viability and chromatin assembly during DNA replication in vertebrate cells.
J Biol Chem. 2006 May 12;281(19):13817-27. Epub 2006 Mar 14.
PMID: 16537536
ASF1A polyclonal antibody ( Cat # PAB7280 ) ( 0.01 µg/ml ) staining of Jurkat cell lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
ASF1A polyclonal antibody ( Cat # PAB7398 ) ( 1 µg/ml ) staining of Jurkat cell lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Human THP-1; WB Suggested Anti-ASF1A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: THP-1 cell lysate; ASF1A antibody - N-terminal region (ARP58260_P050) in Human THP-1 cells using Western Blot
TA302422 (1µg/ml) staining of Jurkat cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA302423 (0.01µg/ml) staining of Jurkat cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of extracts from Jurkat cells, using ASF1A antibody.The lane on the right is treated with the synthesized peptide.
WB Suggested Anti-ASF1A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: THP-1 cell lysate
Western blot analysis on Jurkat cell lysate using ASF1A Antibody
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302422 | ASF1A (C-term) antibody |
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| Goat Polyclonal Antibody against ASF1A | |||||||
 |
Goat | Aff - Purified | Can, Hu, Ms, Rt | WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA302423 | ASF1A antibody |
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| Goat Polyclonal Antibody against ASF1A / HSPC146 | |||||||
 |
Goat | Aff - Purified | Can, Hu, Ms, Rt | WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA312362 | ASF1A antibody |
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| Rabbit polyclonal anti-ASF1A antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA347752 | ASF1A antibody |
||||||
| Rabbit Polyclonal Anti-ASF1A Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP720260 | ASF1A |
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| Recombinant protein of human ASF1 anti-silencing function 1 homolog A (S. cerevisiae) (ASF1A) | |||||
 |
Human | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
10 µg /
€299.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY402268 | ASF1A overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |