ASPH antibody
Principal name
ASPH antibody
Alternative names for ASPH antibody
ASP beta-hydroxylase, HAAH, JCTN, junctin, Aspartyl/asparaginyl beta-hydroxylase, Aspartate beta-hydroxylase, Peptide-aspartate beta-dioxygenase
Ncbi ID
NP_004309, 9606, NP_064549, NP_001158222, NP_115856.1, NM_004318, NP_115855, NP_115857, NP_001158227, NP_001158222.1, NP_001171321.1, NM_023066, NM_001177850, NM_001177853, NM_001177856, NM_133723, NM_001177849, NM_020164, NM_001164756, NM_001164755, NM_001164752, NM_001164754, NM_001164751
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP)
Background of ASPH antibody
This gene is thought to play an important role in calcium homeostasis. The gene is expressed from two promoters and undergoes extensive alternative splicing. The encoded set of proteins share varying amounts of overlap near their N-termini but have substantial variations in their C-terminal domains resulting in distinct functional properties. The longest isoforms (a and f) include a C-terminal Aspartyl/Asparaginyl beta-hydroxylase domain that hydroxylates aspartic acid or asparagine residues in the epidermal growth factor (EGF)-like domains of some proteins, including protein C, coagulation factors VII, IX, and X, and the complement factors C1R and C1S. Other isoforms differ primarily in the C-terminal sequence and lack the hydroxylase domain, and some have been localized to the endoplasmic and sarcoplasmic reticulum. Some of these isoforms are found in complexes with calsequestrin, triadin, and the ryanodine receptor, and have been shown to regulate calcium release from the sarcoplasmic reticulum. Some isoforms have been implicated in metastasis.
General readings
Kwon SJ., et al. (2009) Biochem Biophys Res Commun. 390(4):1389-94.
Human Jurkat; WB Suggested Anti-ASPH Antibody Titration: 5.0ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate; ASPH antibody - middle region (ARP34195_T100) in Human Jurkat cells using Western Blot
Western Blot analysis of ASPH expression in transfected 293T cell line (H00000444-T01) by ASPH MaxPab polyclonal antibody.Lane 1: ASPH transfected lysate(29.81 KDa).Lane 2: Non-transfected lysate.
ASPH MaxPab polyclonal antibody. Western Blot analysis of ASPH expression in HepG2.
Detection limit for recombinant GST tagged ASPH is approximately 1ng/ml as a capture antibody.
Western Blot analysis of ASPH expression in transfected 293T cell line (H00000444-T01) by ASPH MaxPab polyclonal antibody.Lane 1: ASPH transfected lysate(29.81 KDa).Lane 2: Non-transfected lysate.
ASPH MaxPab polyclonal antibody. Western Blot analysis of ASPH expression in HepG2.
Western Blot analysis of ASPH expression in transfected 293T cell line (H00000444-T02) by ASPH MaxPab polyclonal antibody.Lane 1: ASPH transfected lysate(23.1 KDa).Lane 2: Non-transfected lysate.
Human kidney; ASPH antibody - N-terminal region (ARP51357_T100) in Human kidney cells using Immunohistochemistry
Human HepG2; WB Suggested Anti-ASPH Antibody Titration: 2.5ug/ml. Positive Control: HepG2 cell lysate. There is BioGPS gene expression data showing that ASPH is expressed in HepG2.; ASPH antibody - N-terminal region (ARP51357_T100) in Human HepG2 cells using Western Blot
Human 721_B; Host: Rabbit. Target Name: WT1 . Sample Tissue: 721_B . Antibody Dilution: 1.0ug/ml. ASPH is supported by BioGPS gene expression data to be expressed in 721_B.; ASPH antibody - N-terminal region (ARP51356_P050) in Human 721_B cells using Western Blot
Human HepG2; WB Suggested Anti-ASPH Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate. There is BioGPS gene expression data showing that ASPH is expressed in HepG2.; ASPH antibody - N-terminal region (ARP51356_P050) in Human HepG2 cells using Western Blot
Western Blot analysis of ASPH expression in transfected 293T cell line (H00000444-T04) by ASPH MaxPab polyclonal antibody.Lane 1: ASPH transfected lysate(29.81 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of ASPH expression in transfected 293T cell line (H00000444-T03) by ASPH MaxPab polyclonal antibody.Lane 1: ASPH transfected lysate(23.80 KDa).Lane 2: Non-transfected lysate.
Recombinant human ASPH, 75-270 aa, His-tagged: 15% SDS-PAGE (3 µg)
Immunoprecipitation of ASPH transfected lysate using anti-ASPH MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ASPH purified MaxPab mouse polyclonal antibody (B02P) (H00000444-B02P).
Human Small intestine; Immunohistochemistry with Small intestine, myenteric plexus tissue at an antibody concentration of 5ug/ml using anti-ASPH antibody (ARP34691_P050); ASPH antibody - middle region (ARP34691_P050) in Human Small intestine cells using Immunohistochemistry
Human ACHN; WB Suggested Anti-ASPH Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: ACHN cell lysate; ASPH antibody - middle region (ARP34691_P050) in Human ACHN cells using Western Blot
ASPH Antibody (Center) (Cat. #AP50276PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ASPH Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
ASPH Antibody (Center) (Cat. #AP50276PU-N) western blot analysis in A549 cell line lysates (35µg/lane).This demonstrates the ASPH antibody detected the ASPH protein (arrow).
WB Suggested Anti-ASPH Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: MCF7 cell lysate
WB Suggested Anti-ASPH Antibody Titration: 5.0 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysate
WB Suggested Anti-ASPH Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: ACHN cell lysate
Immunohistochemistry with Small intestine, myenteric plexus tissue at an antibody concentration of 5 ug/ml using anti-ASPH antibody
1Human 721_B; Host: Rabbit; Target Name: ASPH; Sample Tissue: 721_B; Antibody Dilution: 1.0 ug/ml ASPH is supported by BioGPS gene expression data to be expressed in 721_B
WB Suggested Anti-ASPH Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysateThere is BioGPS gene expression data showing that ASPH is expressed in HepG2
WB Suggested Anti-ASPH Antibody Titration: 2.5 ug/ml; Positive Control: HepG2 cell lysateThere is BioGPS gene expression data showing that ASPH is expressed in HepG2
Human kidney
WB Suggested Anti-ASPH Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Human Stomach
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP50276PU-N | ASPH (Center) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
 +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP311817 | ASPH (transcript variant 3) |
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| Recombinant protein of human aspartate beta-hydroxylase (ASPH), transcript variant 3 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP316796 | ASPH (transcript variant 1) |
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| Recombinant protein of human aspartate beta-hydroxylase (ASPH), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP323930 | ASPH (transcript variant 2) |
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| Recombinant protein of human aspartate beta-hydroxylase (ASPH), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP328203 | ASPH (transcript variant 11) |
||||
| Purified recombinant protein of Homo sapiens aspartate beta-hydroxylase (ASPH), transcript variant 11. | |||||
 |
Human | > 80 % Preparation: or Add: Recombint proteins was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY418063 | ASPH overexpression lysate |
|
 |
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR224014L2V | Lenti ORF particles, Asph (GFP-tagged) - Mouse aspartate-beta-hydroxylase (Asph), transcript variant 10, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR223585L1V | Lenti ORF particles, Asph (Myc-DDK-tagged) - Mouse aspartate-beta-hydroxylase (Asph), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,370.00
|
|
| OriGene Technologies, Inc. | ||
